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Cytotechnology

, Volume 70, Issue 4, pp 1131–1141 | Cite as

Therapeutic potential of Bama miniature pig adipose stem cells induced hepatocytes in a mouse model with acute liver failure

  • Shuang Zhang
  • Zhiqiang Zhu
  • Yufeng Wang
  • Shi Liu
  • Chenqiong Zhao
  • Weijun Guan
  • Yuhua ZhaoEmail author
Article

Abstract

The role of mesenchymal stem cells (MSCs) in cellular therapy is well recognized in this work. MSCs have advantages of high proliferation, clone formation, multi-lineage differentiation and immunosuppression. Furthermore, adipose-resident MSCs (ADSCs) are extensively employed due to its advantages of abundant source, low cost and simple operation. Many researchers have emphasized the role of adipose-resident MSCs in the development of therapies for liver injury, but few attentions were paid on the use of induced functional hepatocytes. Therefore, in this work the role of adipose-resident MSCs induced functional hepatocytes was mainly investigated. The function of induced hepatocytes by ELISA and the induction rate was confirmed by flow cytometry and evaluated by experimental observations. The induced hepatocytes were firstly transplanted into CCl4-caused liver damage ICR mice by tail vein. After transplantation, both liver fibrosis and function could be improved by hepatocytes, which were examined through histology, immunofluorescence staining, serum profile and biochemical parameters levels. The production of cytokines was then compared with normal mice and injury mice to explore the therapeutic mechanisms of hepatocytes. Finally, the secretions of TGF-β1, IL-6 and IL-10 in hepatocytes transplanted mice were determined and found to be higher than that of the normal and injury mice. The hepatocytes derived from ADSCs were proven to have a great significance in the therapeutic efficacy and clinical settings of liver disease animal models.

Keywords

Adipose tissue Hepatocytes Liver injury Cell therapy 

Notes

Acknowledgements

This research was funded by the National Natural Science Foundation of China (Grant No.: 31472099; 31672404), the Agricultural Science and Technology Innovation Program (ASTIP) (cxgc-ias-01) and the project National Infrastructure of Animal Germplasm Resources (2016 year).

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Shuang Zhang
    • 1
  • Zhiqiang Zhu
    • 1
  • Yufeng Wang
    • 1
  • Shi Liu
    • 1
  • Chenqiong Zhao
    • 1
  • Weijun Guan
    • 2
  • Yuhua Zhao
    • 1
    Email author
  1. 1.Research Center for Sports Scientific Experiment, Harbin Sport UniversityHarbinChina
  2. 2.Department of Animal Genetic Resources, Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingChina

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