Investigating the mincing method for isolation of adipose-derived stem cells from pregnant women fat
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The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery. We used a homogenizer to mince fat and compared the results with those obtained from the traditional cut method involving a sterile scalpel and forceps. Our results showed that our method provides another stable and quality source of stem cells that could be used in cases with a large quantity of fat. Furthermore, we found that pregnancy adipose-derived stem cells (P-ADSCs) could be maintained in vitro for extended periods with a stable population doubling and low senescence levels. P-ADSCs could also differentiate in vitro into adipogenic, osteogenic, chondrogenic, and insulin-producing cells in the presence of lineage-specific induction factors. In conclusion, like human lipoaspirates, adipose tissues obtained from pregnant women contain multipotent cells with better proliferation and showed great promise for use in both stem cell banking studies as well as in stem cell therapy.
KeywordsAdipose-derived stem cells Homogenizer Cell therapy Regenerative medicine
This study was supported by a grant from the Guang Li Biomedicine and the Ching-Kuo Campus of Min-Sheng Hospital. The authors gratefully acknowledge Karthyayani Rajamani, Ting-Yu Lin and Tsung-Yen Ho for their technical contributions.
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest to declare.
- Eto H, Ishimine H, Kinoshita K, Watanabe-Susaki K, Kato H, Doi K, Kuno S, Kurisaki A, Yoshimura KP (2012) Characterization of human adipose tissue-resident hematopoietic cell populations reveals a novel macrophage subpopulation with CD34 expression and mesenchymal multipotency. Stem Cells Dev 22:985–997CrossRefGoogle Scholar
- Faustini M, Bucco M, Chlapanidas T, Lucconi G, Marazzi M, Tosca MC, Gaetani P, Klinger M, Villani S, Ferretti VV, Vigo D, Torre ML (2010) Nonexpanded mesenchymal stem cells for regenerative medicine: yield in stromal vascular fraction from adipose tissues. Tissue Eng C Methods 16:1515–1521CrossRefGoogle Scholar
- Jungblut M, Oeltze K, Zehnter I, Hasselmann D, Bosio A (2008) Preparation of single-cell suspensions from mouse spleen with the gentle MACS dissociator. J Vis Exp pii:1029Google Scholar
- Kuhbier JW, Weyand B, Radtke C, Vogt PM, Kasper C, Reimers K (2010) Isolation, characterization, differentiation, and application of adipose-derived stem cells. Adv Biochem Eng Biotechnol 123:55–105Google Scholar
- Rodbell M (1966) The metabolism of isolated fat cells. IV. Regulation of release of protein by lipolytic hormones and insulin. J Biol Chem 241:3909–3917Google Scholar
- Zimmerlin L, Donnenberg VS, Rubin JP, Donnenberg AD (2012) Mesenchymal markers on human adipose stem/progenitor cells. Cytometry A 83:134–140Google Scholar