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Investigating the mincing method for isolation of adipose-derived stem cells from pregnant women fat

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Abstract

The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery. We used a homogenizer to mince fat and compared the results with those obtained from the traditional cut method involving a sterile scalpel and forceps. Our results showed that our method provides another stable and quality source of stem cells that could be used in cases with a large quantity of fat. Furthermore, we found that pregnancy adipose-derived stem cells (P-ADSCs) could be maintained in vitro for extended periods with a stable population doubling and low senescence levels. P-ADSCs could also differentiate in vitro into adipogenic, osteogenic, chondrogenic, and insulin-producing cells in the presence of lineage-specific induction factors. In conclusion, like human lipoaspirates, adipose tissues obtained from pregnant women contain multipotent cells with better proliferation and showed great promise for use in both stem cell banking studies as well as in stem cell therapy.

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Acknowledgements

This study was supported by a grant from the Guang Li Biomedicine and the Ching-Kuo Campus of Min-Sheng Hospital. The authors gratefully acknowledge Karthyayani Rajamani, Ting-Yu Lin and Tsung-Yen Ho for their technical contributions.

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Authors and Affiliations

  1. Department of Life Science and Graduate Institute of Biotechnology, National Dong Hwa University, No. 1, Sec. 2, Da Hsueh Rd., Shou-Feng, Hualien, Taiwan, ROC

    Yuan-Sheng Li & Tzyy-Wen Chiou

  2. Department of Obstetrics and Gynecology, Ching-Kuo Campus of Min-Sheng Hospital, Taoyuan, Taiwan, ROC

    Pao-Jen Chen

  3. Gene and Stem Cells Production Laboratory Experimental Research Group, Department of Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC

    Li-Wei Wu & Li-Yi Sun

  4. Guang Li Biomedicine, INC., New Taipei City, Taiwan, ROC

    Pei-Wen Chou

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  1. Yuan-Sheng Li
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Correspondence to Tzyy-Wen Chiou.

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Li, YS., Chen, PJ., Wu, LW. et al. Investigating the mincing method for isolation of adipose-derived stem cells from pregnant women fat. Cytotechnology 70, 55–66 (2018). https://doi.org/10.1007/s10616-017-0162-8

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