Skip to main content
SpringerLink
Log in

Therapeutic effect of 28-homobrassinolide on leukotriene synthesis in leukemia cells

  • Original Article
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The present study was aimed to investigate the effect of 28-homobrassinolide on leukotriene synthesis in the 2H3 cells. Cell viability was determined by using sulforhodamine B (SRB) assay. Leukotriene C4 (LTC4) and Leukotriene B4 (LTB4) were determined in the cell and cell lysates. Intracellular Ca2+ was determined in the intact cells. Phospholipases A2 (PLA2) expression was determined in the cells using immunofluorescence. LTC4 and LTB4 were tremendously increased in IgE-loaded 2H3 cells. However, these levels were significantly reduced following treatment with 28-homobrassinolide. The intracellular Ca2+ level was not altered by treatment. Expression of PLA2 was significantly reduced following treatment with 28-homobrassinolide. Taking all these data together, it is suggested that the 28-homobrassinolide may be a potential therapeutic agent to inhibit leukotriene synthesis in 2H3 cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Borgeat P, Samuelsson B (1980) Leukotriene A, isolation from human polymorphonuclear leukocytes. J Biol Chem 225:11828–11831

    Google Scholar 

  • Channon JY, Lesile CC (1990) A calcium-dependent mechanism for associating a soluble arachidonoyl-hydrolyzing phospholipase A2 with the membrane in the macrophage cell line RAW 264.7. J Biol Chem 265:5409–5413

    CAS  Google Scholar 

  • Denis D, Falgueyret JP, Riendeau D, Abramovitz M (1991) Characterization of the activity of purified recombinant human 5-lipoxygenase in the absence and presence of leukocyte factors. J Biol Chem 266:5072–5079

    CAS  Google Scholar 

  • Hamasaki Y, Tai HH (1985) Gossypol, a potent inhibitor of arachidonate 5- and 12-lipoxygenases. Biochim Biophys Acta 834:37–41

    Article  CAS  Google Scholar 

  • Hamasaki Y, Abe M, Matsumoto S, Ichimaru T, Hara N, Miyazaki S (1994) Specific induction of LTC4 synthesis by retinoic acid in rat basophilic leukemia-1 cells. Int Arch Allergy Appl Immunol 103:260–265

    Article  CAS  Google Scholar 

  • Hamasaki Y, Muro E, Miyanji S, Yamamoto S, Kobayashi I, Sato R, Zaitu M, Matsuo M, Ichimaru T, Tasaki H, Miyazaki S (1996) Inhibition of leukotriene synthesis by honokiol in rat basophilic leukemia cells. Int Arch Allergy Appl Immunol 110:278–281

    Article  CAS  Google Scholar 

  • Hojo M, Fujita I, Hamasaki Y, Miyazaki M, Miyazaki S (1994) Erythromycin does not directly affect neutrophil functions. Chest 105:520–523

    Article  CAS  Google Scholar 

  • Hughes CL, Kaldas RS, Weisinger AS (1991) Acute and subacute effects of naturally occurring estrogens on luteinizing hormone secretion in the ovariectomized rat. Reprod Toxicol 5:127–132

    Article  CAS  Google Scholar 

  • Inoue H, Mori T, Shibata S, Koshihara Y (1988) Inhibitory effect of glycyrrhetinic acid derivatives on arachidonic acid-induced mouse ear oedema. J Pharm Pharmacol 40:272–277

    Article  CAS  Google Scholar 

  • Kazan K (2015) Diverse roles of jasmonates and ethylene in abiotic stress tolerance. Trends Plant Sci 20:219–229

    Article  CAS  Google Scholar 

  • Koshihara Y, Neichi T, Murota S, Lao A, Fujimoto Y, Tatsuno T (1984) Caffeic acid is a selective inhibitor of leukotriene biosynthesis. Biochem Biophys Acta 792:92–97

    Article  CAS  Google Scholar 

  • Kumar M, Agnihotri RK, Vamil R, Sharma R (2014) Effect of phytohormones on seed germination and seedling growth of Coriandrum sativum L. Pak J Biol Sci 17:594–596

    Article  Google Scholar 

  • Li XJ, Yang MF, Chen H, Qu LQ, Chen F, Shen SH (2010) Abscisic acid pretreatment enhances salt tolerance of rice seedlings: proteomic evidence. Biochim Biophys Acta 1804:929–940

    Article  CAS  Google Scholar 

  • Marom Z, Shelhamer JH, Back MK, Morton DR, Kaliner M (1982) Slow-reacting substance, leukotriene C4 and D4, increase the release of mucus from human airways in vitro. Am Rev Respir Dis 126:449–451

    CAS  Google Scholar 

  • Matsumoto S, Hamasaki Y, lchimaru T, Miyazaki S (1995) IL-3 and IL-5 enhance the production of LTB4 stimulated by calcium ionophore in rat basophilic leukemia cells. Prostaglandins Leukot Essent Fatty Acids 52:417–422

    Article  CAS  Google Scholar 

  • Muthuraman P, Srikumar K (2009) A comparative study on the effect of homobrassinolide and gibberellic acid on lipid peroxidation and antioxidant status in normal and diabetic rats. J Enzyme Inhib Med Chem 24:1122–1127

    Article  CAS  Google Scholar 

  • Muthuraman P, Srikumar K (2010) Induction of hexokinase I expression in normal and diabetic rats by a brassinosteroid isoform. Eur J Pharm Sci 41:1–9

    Article  CAS  Google Scholar 

  • Muthuraman P, Ravikumar S, Srikumar K (2010) Enhanced expression of hexokinase I mRNA in male rat tissues by homobrassinolide. Prep Biochem Biotechnol 40:256–262

    Article  CAS  Google Scholar 

  • Muthuraman P, Ravikumar S, Muthuviveganandavel V, Dongpil K (2014) Effect of cortisol on calpains in the C2C12 and 3T3-L1 cells. Appl Biochem Biotechnol 172:3153–3162

    Article  CAS  Google Scholar 

  • Muthuraman P, Enkhtaivan G, Kim DH (2016) Cytotoxic effects of aspartame on human cervical carcinoma cells. Toxicol Res 5:45–52

    Article  Google Scholar 

  • Nagajyothi PC, Pandurangan M, Sreekanth TV, Shim J (2016) In vitro anticancer potential of BaCO3 nanoparticles synthesized via the green route. J Photochem Photobiol B 156:29–34

    Article  CAS  Google Scholar 

  • Nakashima S, Fujimiya H, Miyata H, Nozawa Y (1991) Antigen-induced biphasic diacylglycerol formation in RBL-2H3 cells: the late sustained phase due to phosphatidylcholine hydrolysis is dependent on protein kinase C. Biochem Biophs Res Commun 177:336–342

    Article  CAS  Google Scholar 

  • Penrose JF, Spoctor J, Lain BK, Friend DS, Xu K, Jack RM, Austen KF (1995) Purification of human lung leukotriene C4 synthase and preparation of polyclonal antibody. Am J Respir Crit Care Med 152:283–289

    Article  CAS  Google Scholar 

  • Phua DH, Zosel A, Heard K (2009) Dietary supplements and herbal medicine toxicities—when to anticipate them and how to manage them. Int J Emerg Med 2:69–76

    Article  CAS  Google Scholar 

  • Rouzer CA, Ford-Hutchinson AW, Morton HE, Gillard JW (1990) MK-886, a potent specific leukotriene biosynthesis inhibitor blocks and reverses the membrane association of 5-lipoxygenase in ionophore-challenged leukocytes. J Biol Chem 265:1436–1442

    CAS  Google Scholar 

  • Schienvella AR, Regier MK, Smith WL, Lin LL (1995) Calcium-mediated translocation of cytosolic phospholipase A2 to the nuclear envelope and endoplasmic reticulum. J Biol Chem 270:30479–30754

    Article  Google Scholar 

  • Sharma JN, Mohammed LA (2006) The role of leukotrienes in the pathophysiology of inflammatory disorders: Is there a case for revisiting leukotrienes as therapeutic targets? Inflammopharmacology 14:10–16

    Article  CAS  Google Scholar 

  • Srivastava LM (2002) Plant growth and development: hormones and environment. Academic Press, Amsterdam, p 140

    Google Scholar 

  • Ukena D, Fishman L, Niebling W-B (2008) Bronchial asthma: diagnosis and long-term treatment in adults. Dtsch Arztebl Int 105:385–394

    Google Scholar 

  • Vob U, Bishopp A, Farcot E, Bennett MJ (2014) Modelling hormonal response and development. Trends Plant Sci 19:311–319

    Article  Google Scholar 

  • Wasfi IA, Bashir AK, Abdalla AA (1992) Some pharmacological studies on Teucrium mascatense. Effect on glucose homeostasis in normal and streptozotocin diabetic rats and antimicrobial activity. Arab Gulf J Sci Res 10:145–157

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, No. 37 Guo Xue Xiang, Chengdu, 610041, Sichuan, China

    Chuan He, Zhigang Liu, Jingcao Huang & Ting Liu

Authors
  1. Chuan He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhigang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jingcao Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ting Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ting Liu.

Ethics declarations

Conflict of interest

Authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

He, C., Liu, Z., Huang, J. et al. Therapeutic effect of 28-homobrassinolide on leukotriene synthesis in leukemia cells. Cytotechnology 70, 431–438 (2018). https://doi.org/10.1007/s10616-017-0158-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-017-0158-4

Keywords

Search

Navigation