, Volume 68, Issue 6, pp 2745–2751 | Cite as

Static magnetic field controls cell cycle in cultured human glioblastoma cells

  • Seung Chan Kim
  • Wooseok Im
  • Jay Yong Shim
  • Seung-Ki Kim
  • Beom Jin Kim
Short Communication


Magnetic field has been widely used in clinical diagnostics or for clinical treatment and is an important biomedical technology. Glioblastoma multiforme U87 and U251 are models of a fast growing malignant cancer. We focused on cellular level drafting of these cell lines as a time-dependent effect indicator of static magnetic fields (2000 ± 600 Gauss) by using their fast-growing properties. Cell viability showed a significant decrease (p < 0.01). The results coincided with the occurrence of apoptotic signals or protein expression of cyclin B1 and cyclin dependent kinase 1 in a non-apoptotic manner. Cdk1 was decreased in proportion to ankyrin G and cyclin B1 (Chi-square test, p = 0.0366). Our findings suggest that static magnetic stimulation creates a specific cyto-proliferative pattern, rather than producing randomized growth impairment.


Static magnetic field Glioblastoma Viability Cell cycle Specified pattern 



We thank Dr. Sung-hye Park in the Dept. of Pathology at Seoul National University Hospital for advice in cell morphology screening. This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean Government (MSIP) (NRF-20151009350, KOFAC-URP-2009). The authors declare no competing interests.

Supplementary material

10616_2016_9973_MOESM1_ESM.jpg (99 kb)
Supplementary Fig. 1 Diagram of application of static magnetic fields in cultured human glioblastoma cell. The magnets were applied to the bottom of the wells with a distance of 0.1 - 0.3 cm to the cells. The cells were cultured on a plastic shelf (75-T, 24-well and 96-well respectively) 4.0 ± 0.2 cm above the metal shelf in the incubator, so the metal shelf does not influence the magnetic field within the wells. (JPEG 98 kb)


  1. Ast G (2003) Drug-targeting strategies for prostate cancer. Curr Pharm Des 9:455–466CrossRefGoogle Scholar
  2. Byun Y, Thirumamagal BT, Yang W, Eriksson S, Barth RF, Tjarks W (2006) Preparation and biological evaluation of 10B-enriched 3-[5-{2-(2,3-dihydroxyprop-1-yl)-o-carboran-1-yl}pentan-1-yl]thymidine (N5-2OH), a new boron delivery agent for boron neutron capture therapy of brain tumors. J Med Chem 49:5513–5523CrossRefGoogle Scholar
  3. Carter R, Aspy CB, Mold J (2002) The effectiveness of magnet therapy for treatment of wrist pain attributed to carpal tunnel syndrome. J Fam Pract 51:38–40Google Scholar
  4. Chang JE, Khuntia D, Robins HI, Mehta MP (2007) Radiotherapy and radiosensitizers in the treatment of glioblastoma multiforme. Clin Adv Hematol Oncol 5:894–902Google Scholar
  5. Chen R, Nishimura MC, Bumbaca SM, Kharbanda S, Forrest WF, Kasman IM, Greve JM, Soriano RH, Gilmour LL, Rivers CS, Modrusan Z, Nacu S, Guerrero S, Edgar KA, Wallin JJ, Lamszus K, Westphal M, Heim S, James CD, VandenBerg SR, Costello JF, Moorefield S, Cowdrey CJ, Prados M, Phillips HS (2010) A hierarchy of self-renewing tumor-initiating cell types in glioblastoma. Cancer Cell 17:362–375CrossRefGoogle Scholar
  6. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F (1997) Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 27:1–20CrossRefGoogle Scholar
  7. Elgavish A, Prince C, Chang PL, Lloyd K, Lindsey R, Reed R (1998) Osteopontin stimulates a subpopulation of quiescent human prostate epithelial cells with high proliferative potential to divide in vitro. Prostate 35:83–94CrossRefGoogle Scholar
  8. Feelders RA, Hofland LJ, van Aken MO, Neggers SJ, Lamberts SW, de Herder WW, van der Lely AJ (2009) Medical therapy of acromegaly: efficacy and safety of somatostatin analogues. Drugs 69:2207–2226CrossRefGoogle Scholar
  9. Glover DM, Hagan IM, Tavares AA (1998) Polo-like kinases: a team that plays throughout mitosis. Genes Dev 12:3777–3787CrossRefGoogle Scholar
  10. Gridley DS, Pecaut MJ, Nelson GA (2002) Total-body irradiation with high-LET particles: acute and chronic effects on the immune system. Am J Physiol Regul Integr Comp Physiol 282:R677–R688CrossRefGoogle Scholar
  11. Harlow T, Greaves C, White A, Brown L, Hart A, Ernst E (2004) Randomised controlled trial of magnetic bracelets for relieving pain in osteoarthritis of the hip and knee. BMJ 329:1450–1454CrossRefGoogle Scholar
  12. Ibrahim IH (2006) Biophysical Properties of Magnetized Distilled Water. Egypt J Sol 29:363–369Google Scholar
  13. Joshi AA, Shattuck DW, Thompson PM, Leahy RM (2009) A parameterization-based numerical method for isotropic and anisotropic diffusion smoothing on non-flat surfaces. IEEE Trans Image Process 18:1358–1365CrossRefGoogle Scholar
  14. Kim S, Im W (2010) Static magnetic fields inhibit proliferation and disperse subcellular localization of gamma complex protein3 in cultured C2C12 myoblast cells. Cell Biochem Biophys 57:1–8CrossRefGoogle Scholar
  15. Kioi M, Vogel H, Schultz G, Hoffman RM, Harsh GR, Brown JM (2010) Inhibition of vasculogenesis, but not angiogenesis, prevents the recurrence of glioblastoma after irradiation in mice. J Clin Invest 120:694–705CrossRefGoogle Scholar
  16. Knowles JR (1980) Enzyme-catalyzed phosphoryl transfer reactions. Annu Rev Biochem 49:877–919CrossRefGoogle Scholar
  17. Koshikawa N, Minegishi T, Nabeshima K, Seiki M (2008) Development of a new tracking tool for the human monomeric laminin-gamma 2 chain in vitro and in vivo. Cancer Res 68:530–536CrossRefGoogle Scholar
  18. Lacroix M, Abi-Said D, Fourney DR, Gokaslan ZL, Shi W, DeMonte F, Lang FF, McCutcheon IE, Hassenbusch SJ, Holland E, Hess K, Michael C, Miller D, Sawaya R (2001) A multivariate analysis of 416 patients with glioblastoma multiforme: prognosis, extent of resection, and survival. J Neurosurg 95:190–198CrossRefGoogle Scholar
  19. Maher EA, Furnari FB, Bachoo RM, Rowitch DH, Louis DN, Cavenee WK, DePinho RA (2001) Malignant glioma: genetics and biology of a grave matter. Genes Dev 15:1311–1333CrossRefGoogle Scholar
  20. Mayrovitz HN, Groseclose EE (2005) Effects of a static magnetic field of either polarity on skin microcirculation. Microvasc Res 69:24–27CrossRefGoogle Scholar
  21. Patel RR, Mehta MP (2007) Targeted therapy for brain metastases: improving the therapeutic ratio. Clin Cancer Res 13:1675–1683CrossRefGoogle Scholar
  22. Rosen AD (1993) A proposed mechanism for the action of strong static magnetic fields on biomembranes. Int J Neurosci 73:115–119CrossRefGoogle Scholar
  23. Rosen AD (1996) Inhibition of calcium channel activation in GH3 cells by static magnetic fields. Biochim Biophys Acta 1282:149–155CrossRefGoogle Scholar
  24. Rosen AD, Chastney EE (2009) Effect of long term exposure to 0.5 T static magnetic fields on growth and size of GH3 cells. Bioelectromagnetics 30:114–119CrossRefGoogle Scholar
  25. Saleh EM, El-Awady RA, Abdel Alim MA, Abdel Wahab AH (2009) Altered expression of proliferation-inducing and proliferation-inhibiting genes might contribute to acquired doxorubicin resistance in breast cancer cells. Cell Biochem Biophys 55:95–105CrossRefGoogle Scholar
  26. Santamaria D, Barriere C, Cerqueira A, Hunt S, Tardy C, Newton K, Caceres JF, Dubus P, Malumbres M, Barbacid M (2007) Cdk1 is sufficient to drive the mammalian cell cycle. Nature 448:811–815CrossRefGoogle Scholar
  27. Schneider DT, Wessalowski R, Calaminus G, Pape H, Bamberg M, Engert J, Waag K, Gadner H, Gobel U (2001) Treatment of recurrent malignant sacrococcygeal germ cell tumors: analysis of 22 patients registered in the German protocols MAKEI 83/86, 89, and 96. J Clin Oncol 19:1951–1960Google Scholar
  28. Sharma DN, Goyal SG, Muzumder S, Haresh KP, Bahl A, Julka PK, Rath GK (2010) Radiation therapy in paediatric gliomas: our institutional experience. Neurol Neurochir Pol 44:28–34Google Scholar
  29. Tornroth-Horsefield S, Neutze R (2008) Opening and closing the metabolite gate. Proc Natl Acad Sci USA 105:19565–19566CrossRefGoogle Scholar
  30. van de Ven AL, Adler-Storthz K, Richards-Kortum R (2009) Delivery of optical contrast agents using Triton-X100, part 2: enhanced mucosal permeation for the detection of cancer biomarkers. J Biomed Opt 14:021013CrossRefGoogle Scholar
  31. Wen PY (2010) Therapy for recurrent high-grade gliomas: does continuous dose-intense temozolomide have a role? J Clin Oncol 28:1977–1979CrossRefGoogle Scholar
  32. Wright JD, St Clair CM, Deutsch I, Burke WM, Gorrochurn P, Sun X, Herzog TJ (2010) Pelvic radiotherapy and the risk of secondary leukemia and multiple myeloma. Cancer 116:2486–2492CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Seung Chan Kim
    • 1
  • Wooseok Im
    • 2
  • Jay Yong Shim
    • 2
  • Seung-Ki Kim
    • 2
  • Beom Jin Kim
    • 3
  1. 1.Medical School, College of MedicineYonsei UniversitySeoulSouth Korea
  2. 2.Seoul National University HospitalSeoulSouth Korea
  3. 3.Department of MathematicsYonsei UniversitySeoulSouth Korea

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