Abstract
This study assessed the responses of primary cultured haemocytes from the marine gastropod Haliotis tuberculata exposed to the increasing concentrations of industrial effluent (0, 0.5, 1, 10, 15 and 20%) discharged into the Tunisian coastal area. Analyses showed the presence of metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), zinc (Zn), nickel (Ni) and lead (Pb) in the effluent. The effects of this mixture of pollutants on abalone haemocyte parameters were reflected by a significant decrease of cell viability, phagocytotic activity and reactive oxygen species (ROS) production as well as morphological and lysosomal membrane alterations. Thus, these results indicated that our primary culture system represents a suitable in vitro model for monitoring of anthropogenic contaminants in aquatic environments.
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Acknowledgements
This work was supported by a grant from the Ministry of higher education and scientific research, University of Sfax, Tunisia to R. Ladhar-Chaabouni. The authors thank L. Poulain and M. Duval (Plateau Technique de Cytométrieen Flux,Université de Caen Normandie, France) for their helpful technical assistance, the technical staff of the Centre de Recherche en Environnement Côtier (Luc-sur-Mer, Normandie, France) for their assistance in animal care and F. Sdirat for English revision.
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Ladhar-Chaabouni, R., Houel, T., Serpentini, A. et al. Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure. Cytotechnology 69, 191–200 (2017). https://doi.org/10.1007/s10616-016-0050-7
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DOI: https://doi.org/10.1007/s10616-016-0050-7