Skip to main content
SpringerLink
Log in

Assessment of the antimicrobial, antioxidant and cytotoxic activities of the wild edible mushroom Agaricus lanipes (F.H. Møller & Jul. Schäff.) Hlaváček

  • Original Article
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The present investigation was undertaken to evaluate the antimicrobial and antioxidant activities of the wild edible mushroom Agaricus lanipes, and also to investigate its cytotoxicity and potential and possible apoptotic effect against the A549 lung cancer cell line in in vitro conditions. Total antioxidant capacity, total phenolic content, total oxidant status, total antioxidant status, lipid hydroperoxides, and total free –SH levels of A. lanipes were found to be 4.55 mg T/g, 14.6 mg GA equivalent/g, 3.10 mg H2O2 equivalent/g, 2.25 mg H2O2 equivalent/g, and 1.90 µmol/g, respectively. The methanolic extract of A. lanipes had relatively strong antimicrobial activity against seven tested microorganism strains. It also had high anti-proliferative potency and strong pro-apoptotic effects, and this mushroom used as a daily nutrient could be a source for new drug developments and treatment in cancer therapies, and could be a guide for studies in this area.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Alves MJ, Ferreira ICFR, Dias J, Teixeira V, Martins A, Pintado M (2012) A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Planta Med 78:1707–1718

    Article  CAS  Google Scholar 

  • Arab K, Steghens JP (2004) Plasma lipid hydroperoxides measurement by an automated xylenol orange method. Anal Biochem 325:158–163

    Article  CAS  Google Scholar 

  • Barros L, Venturini BA, Baptista P, Estevinho LM, Ferreira ICFR (2008) Chemical composition and biological properties of Portuguese wild mushrooms: a comprehensive study. J Agric Food Chem 56:3856–3862

    Article  CAS  Google Scholar 

  • Chang LW, Yen WJ, Huang SC, Duh PD (2002) Antioxidant activity of sesame coat. Food Chem 78:347–354

    Article  CAS  Google Scholar 

  • Cheung YH, Sheridan CM, Lo AC, Lai WW (2012) Lectin from Agaricus bisporus inhibited S phase cell population and Akt Phosphorylation in human RPE cells. Retinal Cell Biol 53:7469–7475

    CAS  Google Scholar 

  • Collins CH, Lyne PM (1987) Microbiological methods. Butter Worths and Co Ltd, London

    Google Scholar 

  • Elman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77

    Article  Google Scholar 

  • Erel O (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 37:277–285

    Article  CAS  Google Scholar 

  • Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clin Biochem 38:1103–1111

    Article  CAS  Google Scholar 

  • Fan M-J, Lin YC, Shih HD, Ynag HS, Liu KC, Yang St, Lin CY, Wu SC, Yu CS, Ko YC, Chung JG (2011) Crude extracts of Agaricus brasiliensis induce apoptosis in human oral cancer CAL27 cells through a mitocondria-dependent pathway. In vivo 25:355–366

    Google Scholar 

  • Gu YH, Sivam G (2006) Cytotoxic effect of oyster mushroom Pleurotus ostreatus on human androgen independent prostate cancer PC-3 cells. J Med Food 9:196–204

    Article  CAS  Google Scholar 

  • Guerra Dore CMP, Azevedo TCG, de Souza MCR, Leonardo AR, Julio CM, de Dantas JCM, Fernando RFS, Hugo AOR, Luri GB, Edda LL (2007) Antiinflammatory, antioxidant and cytotoxic actions of β-glucan-rich extract from Geastrum saccatum mushroom. Int Immunopharmacol 7:1160–1169

    Article  CAS  Google Scholar 

  • Guto S, Kamada K, Soh Y, Ihara Y, Kondo T (2002) Significance of nuclear glutathione S-transferase pi in resistance to anti-cancer drugs. Jpn J Cancer Res 93:1047–1056

    Article  Google Scholar 

  • Halliwell B (1996) Antioxidants in human health and disease. Annu Rev 16:33–50

    CAS  Google Scholar 

  • Jiahua J, Sliva D (2010) Novel medicinal mushroom blend suppresses growth and invasiveness of human breast cancer cells. Int J Oncol 37:1529–1536

    Google Scholar 

  • Kyslychenko V, Karpiuk U, Diakonova I, Abu-Darwish MS (2010) Phenolic compounds and terpenes in the green parts of Glycine hispida. Adv Environ Biol 4:490–494

    CAS  Google Scholar 

  • Lin JG, Yang JS, Lai KC, Ma CY (2012) An extract of Agaricus blazei Murill administered orally promotes immune responses in murine leukemia Balb/c in vivo. Integr Cancer Ther 11:29–36

    Article  Google Scholar 

  • Lindequist U, Niedermeyer THJ, Julich WD (2005) The pharmacological potential of mushrooms. eCAM 2:285–299

    Google Scholar 

  • Liu K, Wang J, Zhao L, Wang Q (2013) Anticancer, antioxidant and antibiotic activities of mushroom Ramaria flava. Food Chem Toxicol 58:375–380

    Article  CAS  Google Scholar 

  • Mazzutti S, Ferreira SRS, Rieh CAS, Smania A, Smania FA, Martinez J (2012) Supercritical fluid extraction of Agaricus brasiliensis: antioxidant and antimicrobial activities. J Supercrit Fluids 70:48–56

    Article  CAS  Google Scholar 

  • Menikpurage IP, Soysa S, Abeytunga DT (2012) Antioxidant activity and cytotoxicity of the edible mushroom, Pleurotus cystidiosus against Hep-2 carcinoma cells. J Natl Sci Found SRI 40:107–114

    Google Scholar 

  • Moongkardndi P, Kosem N, Kaslungka S, Luanratana O, Pongpan N, Neungton N (2004) Antiproliferation, antioxidation and induction of apoptosis by Garccinia mangostana (mandosteen) on SABR3 human breast cancer cell line. J Ethnopharmacol 90:161–166

    Article  Google Scholar 

  • Niu YC, Liu JC, Zhao XM, Cao J (2009) A low molecular weight polysaccharide isolated from Agaricus blazei Murill (LMPAB) exhibits its anti-metastatic effect by down-regulating metalloproteinase-9 and up-regulating Nm23-H1. Am J Chin Med 37:909–921

    Article  CAS  Google Scholar 

  • Ozturk M, Duru ME, Kivrak S, Mercan-Dogan N, Turkoglu A, Ozler MA (2011) In vitro antioxidant, anticholinesterase and antimicrobial activity studies on three Agaricus species with fatty acid compositions and iron contents: a comparative study on the three most edible mushrooms. Food Chem Toxicol 49:1353–1360

    Article  Google Scholar 

  • Puttaraju NG, Venkateshaiah SU, Dharmesh SM, Urs SMN, Somasundaram R (2006) Antioxidant activity of indigenous edible mushrooms. J Agric Food Chem 54:9764–9772

    Article  CAS  Google Scholar 

  • Robaszkiewicz A, Bartosz G, Lawrynowicz M, Soszynski M (2010) The role of polyphenols, carotene, and lycopene in the antioxidative action of the extracts of dried, edible mushrooms. J Nutr Metab 2010:1–9

    Article  Google Scholar 

  • Rosa A, Rescigno A, Piras A, Atzeri A, Scano P, Porcedda S, Zucco P, Dessi MA (2012) Chemical composition and effect on intestinal Caco-2 cell viability and lipid profile of fixed oil from Cynomorium coccineum L. Food Chem Toxicol 50:3799–3807

    Article  CAS  Google Scholar 

  • Shi YL, Benzie IF, Buswell JA (2002) Role of tyrosinase in the genoprotective effect of the edible mushroom, Agaricus bisporus. Life Sci 70:1595–1608

    Article  CAS  Google Scholar 

  • Skerget M, Kotnik P, Hadolin M, Hra A, Simonic M, Knez Z (2005) Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem 89:191–198

    Article  CAS  Google Scholar 

  • Su ZY, Tung YC, Hwang LS, Sheen LY (2011) Blazeispirol A from Agaricus blazei fermentation product induces cell death in human hepatoma Hep 3B cells through caspase-dependent and caspase-indipendent pathways. J Agric Food Chem 59:5109–5116

    Article  CAS  Google Scholar 

  • Tanaka M, Kuei CW, Nagashima Y, Taguchi T (1998) Application of antioxidative Millard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakk 54:1409–1414

    Article  Google Scholar 

  • Tong H, Xia F, Feng K, Sun G, Gao X, Sun L, Jiang R, Tian D, Sun X (2009) Structural characterization and in vitro antitumor activity of a novel polysaccharide isolated from the fruiting bodies of Pleurotus ostreatus. Bioresour Technol 100:1682–1686

    Article  CAS  Google Scholar 

  • Vaz JA, Heleno SA, Martins A, Almeida GM, Vasconcelos MH, Ferreira ICFR (2010) Wild mushrooms Clitocybe alexandri and Lepista inversa: in vitro antioxidant and growth inhibition of human tumour cell lines. Food Chem Toxicol 48:2881–2884

    Article  CAS  Google Scholar 

  • Wasser SP, Weis AL (1999) Medicinal properties of substances occurring in higher Basidiomycetes mushrooms: current perspective (review). Int J Med Mushrooms 1:31–62

    Article  CAS  Google Scholar 

  • Wayner DDM, Burton GW, Ingold KU, Barclay LC, Locke SJ (1987) The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochim Biophys Acta 924:408–419

    Article  CAS  Google Scholar 

  • Woldegiorgis AZ, Abata D, Haki GD, Ziegler GR (2014) Antioxidant property of edible mushrooms collected from Ethiopia. Food Chem 157:30–36

    Article  CAS  Google Scholar 

  • Wong JH, Ng TB, Cheung RCF, Ye XJ, Wang HX, Lam SK, Lin P, Chan YS, Fang EF, Ngai PHK, Xia LX, Ye XY, Jiang Y, Liu F (2010) Proteins with antifungal properties and other medicinal applications from plants and mushrooms. Appl Microbiol Biotechnol 87:1221–1235

    Article  CAS  Google Scholar 

  • Wu MF, Chen YL, Lee MH, Shih YL, Hsu YM, Tang MC, Lu HF, Tang NY, Yang ST, Chueh FS, Chung JG (2011) Effect of Agaricus blazei Murrill Extract on HT-29 Human colon Cancer Cells in SCID Mice in vivo. In Vivo 25:673–678

    Google Scholar 

  • Wu B, Cui J, Zhang C, Li Z (2012) A polysaccharide from Agaricus blazei inhibits proliferation and promotes apoptosis of osteosarcoma cells. Int J Biol Macromol 50:1116–1120

    Article  CAS  Google Scholar 

  • Yen GC, Duh PD, Tsai CL (1993) Relationship between antioxidant activity and maturity of peanut hulls. J Agric Food Chem 41:67–70

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge the financial support of the Pamukkale University Scientific Research Projects Coordination Unit (PAUBAP) (Project Numbers: 2010FBE086 and 2013KRM008).

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Science and Arts, Pamukkale University, P.O. BOX 20001, Denizli, Turkey

    Oğuzhan Kaygusuz & Kutret Gezer

  2. Denizli Vocational School of Technical Sciences, Pamukkale University, Denizli, Turkey

    Meruyert Kaygusuz

  3. Department of Medical Biology, Faculty of Medicine, Pamukkale University, Denizli, Turkey

    Yavuz Dodurga & Mücahit Seçme

  4. Department of Food Engineering, Engineering Faculty, Pamukkale University, Denizli, Turkey

    Emine Nur Herken

Authors
  1. Oğuzhan Kaygusuz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meruyert Kaygusuz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yavuz Dodurga
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mücahit Seçme
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Emine Nur Herken
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kutret Gezer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Oğuzhan Kaygusuz.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kaygusuz, O., Kaygusuz, M., Dodurga, Y. et al. Assessment of the antimicrobial, antioxidant and cytotoxic activities of the wild edible mushroom Agaricus lanipes (F.H. Møller & Jul. Schäff.) Hlaváček. Cytotechnology 69, 135–144 (2017). https://doi.org/10.1007/s10616-016-0045-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-016-0045-4

Keywords

Search

Navigation