, Volume 68, Issue 4, pp 565–577 | Cite as

Factors governing the immunosuppressive effects of multipotent mesenchymal stromal cells in vitro

  • Aleksandra Gornostaeva
  • Elena Andreeva
  • Ludmila Buravkova


Multipotent mesenchymal stem/stromal cells (MSCs) are of great interest to researchers because of the unique properties, such as enhanced proliferation, paracrine activity and multilineage differentiation. Their non-immunogenicity, in combination with immunomodulatory properties, opens up the opportunity for the allogeneic application of MSCs. The MSC immunomodulatory capacity is currently being actively studied in vitro using various experimental designs. However, the results are not always univocal. It was found that the outcome of the stromal/immune cell interaction depends on experimental conditions. In this review we considered the impact of different factors, such as the ratio of stromal/immune cells, interaction time, the path of immune cell activation, etc. on the MSC immunomodulation. We also accentuated the importance of local milieu, in particular, oxygen tension, for the realization of MSC immunosuppressive activity.


MSCs Lymphocytes Activation Low oxygen Cell-to-cell interaction Immunosuppression 



This study was funded by Grant RFBR 13-04-00791, Grant RFBR 14-04-31362 mol_a and grant of the President of the Russian Federation SP-3502.2015.4.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Aleksandra Gornostaeva
    • 1
  • Elena Andreeva
    • 1
  • Ludmila Buravkova
    • 1
  1. 1.Institute of Biomedical Problems of RASMoscowRussia

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