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Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

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Abstract

To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed. First an individual biomaterial is selected and then mounted in a MINUSHEET® tissue carrier. After sterilization the assembly is transferred by fine forceps to a 24 well culture plate for seeding cells or mounting tissue on it. To support spatial (3D) development a carrier can be placed in various types of perfusion culture containers. In the basic version a constant flow of culture medium provides contained tissue with always fresh nutrition and respiratory gas. For example, epithelia can be transferred to a gradient container, where they are exposed to different fluids at the luminal and basal side. To observe development of tissue under the microscope, in a different type of container a transparent lid and base are integrated. Finally, stem/progenitor cells are incubated in a container filled by an artificial interstitium to support spatial development. In the past years the described system was applied in numerous own and external investigations. To present an actual overview of resulting experimental data, the present paper was written.

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Acknowledgments

The authors thank the Institute of Molecular and Cellular Anatomy, University of Regensburg for financial support and technical assistance to write this article.

Conflict of interest

A series of patents (DE 10 2004 054 125, DE 39 23 279, DE 42 00 446, DE 42 08 805, DE 44 43 902, DE 19 530 556, DE 196 48 876 C2, DE 199 52 847 B4, US 5 190 878, US 5 316 945, US 5 665 599, J 2847669, DE 10 2005 002 938, PA 10 2005 001 747.9) demonstrate that Will W. Minuth is the inventor of the MINUSHEET® perfusion culture system. W. W. Minuth and L. Denk declare no competing interests or financial conflicts.

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  1. Molecular and Cellular Anatomy, University of Regensburg, University Street 31, 93053, Regensburg, Germany

    Will W. Minuth & Lucia Denk

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  1. Will W. Minuth
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Correspondence to Will W. Minuth.

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Minuth, W.W., Denk, L. Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system. Cytotechnology 68, 179–196 (2016). https://doi.org/10.1007/s10616-015-9873-x

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