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NT-3 attenuates the growth of human neuron cells through the ERK pathway

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Abstract

Spinal cord injury is a devastating health problem that affects thousands of individuals each year. The neurons were destroyed. NT-3 is a recently discovered neurotrophin. This study sought to understand the potential involvement of MAPKs in NT-3-mediated growth inhibition of human neurons. We applied different concentrations of NT-3 and observed the growth rate of the cells and the changes in the phosphorylation state of the MAPKs ERK1/2, JNK and p38. This study discovered that NT-3-induced HNC growth was promoted primarily by phosphorylated ERK1/2, and that this phosphorylation, as well p90rskphosphorylation, was mediated by TrkC. The ERK1/2 pathway is known to play an essential role in the NT-3-mediated growth of human neurons. In conclusion, our study suggests that NT-3 promotes the growth of human neurons cells primarily through the TrkC/ERK pathway.

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Acknowledgments

This work was supported by Grant No. 81450037 from the Chinese National Natural Science Foundation and baiwan project of Inner Mongolia Medical University.

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Authors and Affiliations

  1. Department of Orthopedic, The First Affiliated Hospital of Chongqing Medical University, Yuzhong District Yueyuan Road No1, Chongqing, China

    Ruifeng Li & Dianming Jiang

  2. Department of Cervical Surgery, The Second Affiliated Hospital of Inner Mongolia Medical University, Muslims camp Square Road No 1, Hohhot, China

    Ruifeng Li & Yimin Wu

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  1. Ruifeng Li
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  2. Yimin Wu
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Correspondence to Dianming Jiang.

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Li, R., Wu, Y. & Jiang, D. NT-3 attenuates the growth of human neuron cells through the ERK pathway. Cytotechnology 68, 659–664 (2016). https://doi.org/10.1007/s10616-014-9813-1

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