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Comparative cytogenetic analysis of marine needlefishes (Beloniformes) from southern Brazil

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Abstract

Cytogenetic studies have assisted in the taxonomic classification of organisms, especially those involving species with highly similar morphologic characteristics, or so-called cryptic species. Strongylura marina and Strongylura timucu collected from Paranaguá Bay, Paraná Coast in Southern Brazil are considered cryptic species, and the identification of interspecific variations based on the number and/or morphology of its chromosomes may serve as differentiating cytotaxonomic markers. Chromosomes of the two species were subjected to different banding and staining methods (C-, Ag-, and DAPI-CMA3), as well as chromosomal mapping of major rDNA (45S), revealed with an 18S probe by fluorescence in situ hybridization (FISH). The pattern of distribution of constitutive heterochromatin showed distinct features involving the pericentromeric and telomeric bands in both species. In S. marina, chromosome 1 represents the main species-specific marker, appearing almost entirely heterochromatic. In both species, the 45S rDNA is located at terminal region of the short arm of the chromosome 6, as detected by silver nitrate staining and FISH. Despite the apparent conserved diploid number of 48 chromosomes, data on the karyotype microstructure characterize the cytogenetic profile of the genus and may allow the establishment of cytotaxonomic and evolutionary inferences for these fishes.

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References

  • Affonso PRAM, Galetti PM (2005) Chromosomal diversification of reef fishes from genus Centropyge (Perciformes, Pomacanthidae). Genetica 123:227–233

    Article  CAS  Google Scholar 

  • Arai R (2011) Fish karyotypes: a check list. Springer, Tokyo, p 345

    Book  Google Scholar 

  • Araújo WC, Martinez PA, Molina WF (2010) Mapping of ribosomal DNA by FISH, EcoRI digestion and replication bands in the cardinal fish Apogon americanus (Perciformes). Cytologia 75:109–117

    Article  Google Scholar 

  • Born GG, Bertollo LAC (2000) An XX/XY sex chromosome system in a fish species, Hoplias malabaricus, with a polymorphic NOR-bearing X chromosome. Chromosome Res 8:111–118

    Article  CAS  Google Scholar 

  • Cipriano RR, Fenocchio AS, Artoni RF, Molina WF, Noleto RB, Kantek DLZ, Cestari MM (2008) Chromosomal studies of five species of the marine fishes from the Paranaguá Bay and the karyotypic diversity in the Marine Teleostei of the Brazilian Coast. Braz Arch Biol Technol 51:303–314

    Article  Google Scholar 

  • Collette BB (2003) Family belonidae bonaparte 1932—needlefishes. Calif Acad Sci Annot Checkl Fish 16:1–22

    Google Scholar 

  • Collette BB, Mcgowen GE, Parin NV, Mito S (1984) Beloniformes: development and relationships. In: Moser HG (ed) Ontogeny and systematics of fish: Am. Soc. Ichthyol. Herpetol. Spec. Publ. 1

  • Dover GA (1986) Molecular drive in multigene families: how biological novelties arise, spread and are assimilated. Trends Genet 2:159–165

    Article  CAS  Google Scholar 

  • Fenocchio AS, Venere PC, Cesar ACG, Dias AL, Bertollo LAC (1991) Short term culture from solid tissues of fishes. Caryologia 44:161–166

    Article  Google Scholar 

  • Galetti PM, Molina WF, Affonso PRAM, Aguilar CT (2006) Assessing genetic diversity of Brazilian reef fishes by chromosomal and DNA markers. Genetica 126:161–177

    Article  Google Scholar 

  • Gottlieb S, Esposito RE (1989) A new role for a yeast transcriptional silencer gene, SIR2, in regulation of recombination in ribosomal DNA. Cell 56:771–776

    Article  CAS  Google Scholar 

  • Goulding M, Carvalho ML (1984) Ecololgy of amazoniam needlefishes (Belonidae). Revista Brasileira de Zoologia 2:99–111

    Google Scholar 

  • Hatanaka T, Galetti PM (2004) Mapping of the 18S and 5S ribosomal RNA genes in the Prochilodus argenteus Agassiz, 1829 (Characiformes, Prochilodontidae). Genetica 12:239–244

    Article  Google Scholar 

  • Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developper a 1-step method. Experientia 36:1014–1015

    Article  CAS  Google Scholar 

  • Jaillon O, Aury JM, Brunet F, Petit JL, Stange-Thomann N, Mauceli E, Bouneau L, Fischer C, Ozouf-Costaz C, Bernot A, Nicaud S, Jaffe D, Fisher S, Lutfalla G, Dossat C, Segurens B, Dasilva C, Salanoubat M, Levy M, Boudet N, Castellano S, Anthouard V, Jubin C, Castelli V, Katinka M, Vacherie B, Biémont C, Skalli Z, Cattolico L, Poulain J, De Berardinis V, Cruaud C, Duprat S, Brottier P, Coutanceau JP, Gouzy J, Parra G, Lardier G, Chapple C, McKernan KJ, McEwan P, Bosak S, Kellis M, Volff JN, Guigó R, Zody MC, Mesirov J, Lindblad-Toh K, Birren B, Nusbaum C, Kahn D, Robinson-Rechavi M, Laudet V, Schachter V, Quétier F, Saurin W, Scarpelli C, Wincker P, Lander ES, Weissenbach J, Roest Crollius H (2004) Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype. Nature 431:946–957

  • John B, King M, Schweizer D, Mendelak M (1985) Equilocality of heterochromatin distribution and heterochromatin heterogeneity in acridid grasshoppers. Chromosoma 91:185–200

    Article  CAS  Google Scholar 

  • Kantek DLZ, Vicari MR, Peres WAM, Cestari MM, Artoni RF, Bertollo LAC, Moreira-Filho O (2009) Chromosomal location and distribution of As51 satellite DNA in five species of the genus Astyanax (Teleostei, Characidae, Incertae sedis). J Fish Biol 75:408–421

    Article  CAS  Google Scholar 

  • Levan A, Fredga K, Sandberg A (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220

    Article  Google Scholar 

  • Liao JC (2002) Swimming in needlefish (Belonidae): anguilliform locomotion with fins. J Exp Biol 205:2875–2884

    Google Scholar 

  • Mazzuchelli J, Martins C (2009) Genomic organization of repetitive DNAs in the cichlid fish Astronotus ocellatus. Genetica 136:461–469

    Article  CAS  Google Scholar 

  • Molina WF, Galetti PM (2007) Early replication banding in Leporinus species (Osteichthyes, Characiformes) bearing differentiated sex chromosomes (ZW). Genetica 130:153–160

    Article  Google Scholar 

  • Molina WF, Schmid M, Galetti PM (1998) Heterochromatin and sex chromosomes in the Neotropical fish genus Leporinus (Characiformes, Anastomidae). Cytobios 94:141–149

    Google Scholar 

  • Naruse K, Tanaka M, Mita K, Shima A, Postlethwait J, Mitani H (2004) A medaka gene map: the trace of ancestral vertebrate proto-chromosomes revealed by comparative gene mapping. Genome Res 14:820–828

    Article  CAS  Google Scholar 

  • Noleto RB, Vicari MR, Cestari MM, Artoni RF (2012) Variable B chromosomes frequencies between males and females of two species of pufferfishes (Tetraodontiformes). Rev Fish Biol Fish 22:343–349

    Article  Google Scholar 

  • Ohno S, Wolf U, Atkin NB (1968) Evolution from fish to mammals by gene duplication. Hereditas 59:169–187

    Article  CAS  Google Scholar 

  • Pastori MC, Cano J, Bertollo LAC, Fenocchio AS (1998) Cytogenetic study of two species needlefish (Belonidae) from Argentina. Ital J Zool 65:57–60

    Article  Google Scholar 

  • Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938

    Article  CAS  Google Scholar 

  • Rish KK (1973) A preliminary report on the karyotypes of eighteen marine fishes. Res Bull Punjab Univ 24:161–162

    Google Scholar 

  • Rishi KK, Singh J (1982) Karyological studies on five estuarine fishes. Nucleus 25:178–180

    Google Scholar 

  • Saito Y, Edpalina RR, Abe S (2007) Isolation and characterization of salmonid telomeric and centromeric satellite DNA sequences. Genetica 131:157–166

    Article  CAS  Google Scholar 

  • Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58:307–324

    Article  CAS  Google Scholar 

  • Sczepanski TS, Noleto RB, Cestari MM, Artoni RF (2010) A comparative study of two marine catfish (Siluriformes, Ariidae): cytogenetic tools for determining cytotaxonomy and karyotype evolution. Micron 41:193–197

    Article  CAS  Google Scholar 

  • Srivastava MDL, Kaura P (1964) The structure and behaviour of chromosomes in six freshwater teleosts. Cellule 65:93–107

    CAS  Google Scholar 

  • Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304–306

    Article  CAS  Google Scholar 

  • Vicari MR, Noleto RB, Artoni RF, Moreira-Filho O, Bertollo LAC (2008) Comparative cytogenetics among species of the Astyanax scabripinnis complex. Evolutionary and biogeographical inferences. Genet Mol Biol 31:173–179

    Article  CAS  Google Scholar 

  • Vicari MR, Nogaroto V, Noleto RB, Cestari MM, Cioffi MB, Almeida MC, Moreira-Filho O, Bertollo LA, Artoni RF (2010) Satellite DNA and chromosomes in neotropical fishes: methods, applications and perspectives. J Fish Biol 76:1094–1116

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Acknowledgments

We thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná for the financial support for this study.

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Authors and Affiliations

  1. Departamento de Fitotecnia e Fitossanitarismo, Setor de Ciências Agrárias, Universidade Federal do Paraná, Rua dos Funcionários, 1540, Curitiba, Paraná, CEP: 80035-050, Brazil

    Roger Raupp Cipriano

  2. Departamento de Biologia, Universidade Estadual do Paraná, Campus União da Vitória, Praça Coronel Amazonas, União da Vitória, Paraná, CEP: 84600-000, Brazil

    Rafael Bueno Noleto

  3. Taiamã Ecological Station, Instituto Chico Mendes de Conservação da Biodiversidade, Cáceres, Mato Grosso, Brazil

    Daniel Luis Zanella Kantek

  4. Laboratório de Crustáceos Decápodes, Instituto de Oceanografia, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil

    Maria Cristina da Silva Cortinhas

  5. Departamento de Genética, Universidade Federal do Paraná, Centro Politécnico, Curitiba, Paraná, CEP: 81530-900, Brazil

    Marta Margarete Cestari

Authors
  1. Roger Raupp Cipriano
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  2. Rafael Bueno Noleto
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  3. Daniel Luis Zanella Kantek
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  4. Maria Cristina da Silva Cortinhas
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  5. Marta Margarete Cestari
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Corresponding authors

Correspondence to Roger Raupp Cipriano or Rafael Bueno Noleto.

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Cipriano, R.R., Noleto, R.B., Kantek, D.L.Z. et al. Comparative cytogenetic analysis of marine needlefishes (Beloniformes) from southern Brazil. Cytotechnology 68, 1585–1589 (2016). https://doi.org/10.1007/s10616-014-9809-x

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  • DOI: https://doi.org/10.1007/s10616-014-9809-x

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