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Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs

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Abstract

Elicitation effect of silver nano particles (AgNPs) and triggering of defence system by production of hydrogen peroxide (H2O2) as a signaling molecule in the regulation of the activity of stress-related enzymes and production of Taxol was evaluated in suspension- cultured hazel cells (Corylus avellana L.). The cells were treated with different concentrations of AgNPs (0, 2.5, 5, and 10 ppm), in their logarithmic growth phase (d7) and were harvested after 1 week. Treatment of hazel cells with AgNPs decreased the viability of the cells. Also the results showed that while the activity of certain radical scavenging enzymes in particular of catalase and peroxidase increased by 2.5 and 5 ppm AgNPs, the activity of superoxide dismutase decreased in these treatments. The highest activity of ascorbate peroxidase was observed in 10 ppm AgNPs treatments. This treatment also showed the highest contents of H2O2 and phenolic compounds, as well as the highest activity of phenylalanine ammonialyase. According to the results, 5 ppm AgNPs was the best concentration for elicitation of hazel cells to produce efficient amounts of H2O2 in order for stimulation of antioxidant defence system, production of Taxol at the highest capacity of the cells, meanwhile reserving their viability.

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Abbreviations

AgNPs:

Silver nano particles

APX:

Ascorbate peroxidase

CAT:

Catalase

H2O2 :

Hydrogen peroxide

NBT:

Nitroblue tetrazolium

PAL:

Phenylalanine ammonia lyase

POD:

Peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgments

This work was supported by grant no. 90000972 from the Iran National Science Foundation (INSF) to FGH on “The effects of MeJA and SNPs on medicinal compounds of plants”.

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Authors and Affiliations

  1. Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), P.O. Box 14115-154, Tehran, Iran

    Mitra Jamshidi, Faezeh Ghanati & Ebrahim Bemani

  2. Department of Agricultural Biotechnology, Faculty of Agriculture, Shahed University, Tehran, Iran

    Ayatollah Rezaei

Authors
  1. Mitra Jamshidi
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  2. Faezeh Ghanati
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  3. Ayatollah Rezaei
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  4. Ebrahim Bemani
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Corresponding author

Correspondence to Faezeh Ghanati.

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Jamshidi, M., Ghanati, F., Rezaei, A. et al. Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs. Cytotechnology 68, 525–530 (2016). https://doi.org/10.1007/s10616-014-9808-y

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  • DOI: https://doi.org/10.1007/s10616-014-9808-y

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