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Implication of SUMO E3 ligases in nucleotide excision repair

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Abstract

Post-translational modifications alter protein function to mediate complex hierarchical regulatory processes that are crucial to eukaryotic cellular function. The small ubiquitin-like modifier (SUMO) is an important post-translational modification that affects transcriptional regulation, nuclear localization, and the maintenance of genome stability. Nucleotide excision repair (NER) is a very versatile DNA repair system that is essential for protection against ultraviolet (UV) irradiation. The deficiencies in NER function remarkably increase the risk of skin cancer. Recent studies have shown that several NER factors are SUMOylated, which influences repair efficiency. However, how SUMOylation modulates NER has not yet been elucidated. In the present study, we performed RNAi knockdown of SUMO E3 ligases and found that, in addition to PIASy, the polycomb protein Pc2 affected the repair of cyclobutane pyrimidine dimers. PIAS1 affected both the removal of 6-4 pyrimidine pyrimidone photoproducts and cyclobutane pyrimidine dimers, whereas other SUMO E3 ligases did not affect the removal of either UV lesion.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Maasa Tsuge, Hidenori Kaneoka, Yusuke Masuda, Hiroki Ito, Katsuhide Miyake & Shinji Iijima

Authors
  1. Maasa Tsuge
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  2. Hidenori Kaneoka
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  3. Yusuke Masuda
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  4. Hiroki Ito
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  5. Katsuhide Miyake
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  6. Shinji Iijima
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Corresponding author

Correspondence to Hidenori Kaneoka.

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Tsuge, M., Kaneoka, H., Masuda, Y. et al. Implication of SUMO E3 ligases in nucleotide excision repair. Cytotechnology 67, 681–687 (2015). https://doi.org/10.1007/s10616-014-9762-8

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  • DOI: https://doi.org/10.1007/s10616-014-9762-8

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