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Assessment of genotoxicity associated with Behcet’s disease using sister-chromatid exchange assay: vitamin E versus mitomycin C

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Abstract

Behcet’s disease (BD) is a multisystemic chronic inflammatory disorder that presents throughout the world with high frequency in Turkey and Middle East. BD has been shown to be associated with genotoxicity as patients with the disease have demonstrated high rates of sister chromatid exchange (SCE) and oxidative DNA damage. In this study, we examined the effect of vitamin E, which is known for its strong antioxidant activity, on the rate of SCE in cultured lymphocytes obtained from BD patients. In addition, the susceptibility of patient lymphocytes to the mutagenic agent mitomycin C (MMC) was also investigated. The results showed significant elevation in the rate of SCE in lymphocytes obtained from patients compared to those from healthy subjects (P < 0.01). Treatment with vitamin E normalized the elevated rate of SCE to a comparable level observed in the control group (P < 0.01). Finally, treatment of cultures with MMC significantly increased the rate of SCE in the lymphocytes of both patients and controls (P < 0.001). The magnitude of change in the rate of SCE induced by MMC was equivalent in both groups. This result suggests similar sensitivity of BD lymphocytes and control ones to MMC. In conclusion, genotoxicity associated with BD can be overcome by treatment with vitamin E. Lymphocytes of BD have normal sensitivity to the mutagenic agent MMC.

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Abbreviations

BD:

Behcet’s disease

MMC:

Mitomycin C

SCE:

Sister chromatid exchange

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Acknowledgments

This work has been done with funds from the Research Deanship at Jordan University of Science and Technology, Grant Number (JUST-171) to OK.

Conflict of interest

The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Department of Medical Laboratory Sciences, Jordan University of Science and Technology, P.O. Box 3030, Irbid, 22110, Jordan

    Omar F. Khabour, Etizaz Al-Kofahi & Fahmee Mesmar

  2. Department of Internal Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Khaldon Alawneh

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  1. Omar F. Khabour
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  2. Khaldon Alawneh
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  3. Etizaz Al-Kofahi
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  4. Fahmee Mesmar
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Correspondence to Omar F. Khabour.

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Khabour, O.F., Alawneh, K., Al-Kofahi, E. et al. Assessment of genotoxicity associated with Behcet’s disease using sister-chromatid exchange assay: vitamin E versus mitomycin C. Cytotechnology 67, 1051–1057 (2015). https://doi.org/10.1007/s10616-014-9744-x

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