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Potential cytotoxic effect of Anilofos by using Allium cepa assay

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Abstract

Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC50 value was determined 50 ppm and 1/2× EC50 (25 ppm), EC50 (50 ppm) and 2 × EC50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase–telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase–telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Sciences, Afyon Kocatepe University, Afyonkarahisar, Turkey

    Arzu Özkara & Dilek Akyıl

  2. Department of Science, Faculty of Education, Suleyman Demirel University, Isparta, Turkey

    Yasin Eren

  3. Department of Veterinary Sciences, Bayat Vocational High School, Afyon Kocatepe University, Afyonkarahisar, Turkey

    S. Feyza Erdoğmuş

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  1. Arzu Özkara
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  2. Dilek Akyıl
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  3. Yasin Eren
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  4. S. Feyza Erdoğmuş
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Correspondence to Arzu Özkara.

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Özkara, A., Akyıl, D., Eren, Y. et al. Potential cytotoxic effect of Anilofos by using Allium cepa assay. Cytotechnology 67, 783–791 (2015). https://doi.org/10.1007/s10616-014-9716-1

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