Abstract
Siglecs, sialic acid-recognizing Ig-superfamily lectins, regulate various aspects of immune responses, and have also been shown to induce the endocytosis of binding materials such as anti-Siglec antibodies or sialic acid-harboring bacteria. In this study, we demonstrated that the expression of Siglec-9 enhanced the transfection efficiency of several cell lines such as macrophage RAW264 and non-hematopoietic 293FT cells. We applied this finding to the production of a lentiviral vector in which cells were transfected simultaneously with multiple vectors, and achieved a twice increase in viral production levels. Furthermore, 293FT cells expressing lectin-defective Siglec-9 produced three- to seven-fold higher titer of viral vector compared with parental 293FT cells. These results suggest that Siglec-9 enhanced lentiviral vector production in a lectin-independent manner.
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References
Ando M, Tu W, Nishijima K, Iijima S (2008) Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs. Biochem Biophys Res Commun 369(3):878–883
Avril T, Freeman SD, Attrill H, Clarke RG, Crocker PR (2005) Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. J Biol Chem 280(20):19843–19851
Cheng PW (1996) Receptor ligand-facilitated gene transfer: enhancement of liposome-mediated gene transfer and expression by transferrin. Hum Gene Ther 7(3):275–282
Crocker PR, McMillan SJ, Richards HE (2012) CD33-related siglecs as potential modulators of inflammatory responses. Ann N Y Acad Sci 1253:102–111
Elfinger M, Maucksch C, Rudolph C (2007) Characterization of lactoferrin as a targeting ligand for nonviral gene delivery to airway epithelial cells. Biomaterials 28(23):3448–3455
Ikehara Y, Ikehara SK, Paulson JC (2004) Negative regulation of T cell receptor signaling by Siglec-7 (p70/AIRM) and Siglec-9. J Biol Chem 279:43117–43125
Jones C, Virji M, Crocker PR (2003) Recognition of sialylated meningococcal lipopolysaccharide by siglecs expressed on myeloid cells leads to enhanced bacterial uptake. Mol Microbiol 49:1213–1225
Kikuchi A, Sugaya S, Ueda H, Tanaka K, Aramaki Y, Hara T, Arima H, Tsuchiya S, Fuwa T (1996) Efficient gene transfer to EGF receptor overexpressing cancer cells by means of EGF-labeled cationic liposomes. Biochem Biophys Res Commun 227:666–671
Kodama D, Nishimiya D, Nishijima K, Okino Y, Inayoshi Y, Kojima Y, Ono K, Motono M, Miyake K, Kawabe Y, Kyogoku K, Yamashita T, Kamihira M, Iijima S (2012) Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the tet expression system. J Biosci Bioeng 113:146–153
Kovacs T, Karasz A, Szollosi J, Nagy P (2009) The density of GM1-enriched lipid rafts correlates inversely with the efficiency of transfection mediated by cationic liposomes. Cytometry A 75:650–657
Kunath K, Merdan T, Hegener O, Haberlein H, Kissel T (2003) Integrin targeting using RGD-PEI conjugates for in vitro gene transfer. J Gene Med 5:588–599
Lajaunias F, Dayer JM, Chizzolini C (2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling. Eur J Immunol 35:243–251
Motono M, Yamada Y, Hattori Y, Nakagawa R, Nishijima K, Iijima S (2010) Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector. J Biosci Bioeng 109:315–321
Nicoll G, Avril T, Lock K, Furukawa K, Bovin N, Crocker PR (2003) Ganglioside GD3 expression on target cells can modulate NK cell cytotoxicity via siglec-7-dependent and -independent mechanisms. Eur J Immunol 33:1642–1648
Orr SJ, Morgan NM, Elliott J, Burrows JF, Scott CJ, McVicar DW, Johnston JA (2007) CD33 responses are blocked by SOCS3 through accelerated proteasomal-mediated turnover. Blood 109:1061–1068
Paul SP, Taylor LS, Stansbury EK, McVicar DW (2000) Myeloid specific human CD33 is an inhibitory receptor with differential ITIM function in recruiting the phosphatases SHP-1 and SHP-2. Blood 96:483–490
Pilatte Y, Bignon J, Lambré CR (1993) Sialic acids as important molecules in the regulation of the immune system: pathophysiological implications of sialidases in immunity. Glycobiology 3:201–218
Rao GA, Tsai R, Roura D, Hughes JA (2008) Evaluation of the transfection property of a peptide ligand for the fibroblast growth factor receptor as part of PEGylated polyethylenimine polyplex. J Drug Target 16:79–89
Scott ES, Wiseman JW, Evans MJ, Colledge WH (2001) Enhanced gene delivery to human airway epithelial cells using an integrin-targeting lipoplex. J Gene Med 3:125–134
Tateno H, Li HY, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC (2007) Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol 27:5699–5710
Ulyanova T, Blasioli J, Woodford-Thomas TA, Thomas ML (1999) The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol 29:3440–3449
van der Velden VH, te Marvelde JG, Hoogeveen PG, Bernstein ID, Houtsmuller AB, Berger MS, van Dongen JJ (2001) Targeting of the CD33-calicheamicin immunoconjugate Mylotarg (CMA-676) in acute myeloid leukemia: in vivo and in vitro saturation and internalization by leukemic and normal myeloid cells. Blood 97:3197–3204
Varki A, Angata T (2006) Siglecs—the major subfamily of I-type lectins. Glycobiology 16:1R–27R
Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T (2004) Cre-lox-regulated conditional RNA interference from transgenes. Proc Natl Acad Sci USA 101(28):10380–10385
von Gunten S, Yousefi S, Seitz M, Jakob SM, Schaffner T, Seger R, Takala J, Villiger PM, Simon HU (2005) Siglec-9 transduces apoptotic and nonapoptotic death signals into neutrophils depending on the proinflammatory cytokine environment. Blood 106(4):1423–1431
Walter RB, Raden BW, Zeng R, Hausermann P, Bernstein ID, Cooper JA (2008) ITIM-dependent endocytosis of CD33-related Siglecs: role of intracellular domain, tyrosine phosphorylation, and the tyrosine phosphatases, Shp1 and Shp2. J Leukoc Biol 83(1):200–211
Zhang M, Angata T, Cho JY, Miller M, Broide DH, Varki A (2007) Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Blood 109(10):4280–4287
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This work was partly supported by the Takeda Foundation, Mishima Foundation, and Terumo Foundation.
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Shoji, T., Higuchi, H., Zaitsu, Y. et al. Enhanced lentiviral vector production in 293FT cells expressing Siglec-9. Cytotechnology 67, 593–600 (2015). https://doi.org/10.1007/s10616-013-9679-7
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DOI: https://doi.org/10.1007/s10616-013-9679-7