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Comparative cytogenetics between two species of the family Pseudopimelodidae (Siluriformes): occurrence of natural triploidy and supernumerary chromosomes

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Abstract

The Pseudopimelodidae family comprises 35 species however, cytogenetic studies have been performed in only six species. This study uncovered karyotypic data on Pseudopimelodus pulcher and Microglanis cottoides. Both species possessed 2n = 54, with 20m + 16sm + 10st + 8a and FN = 100 for P. pulcher and 30m + 14sm + 6st + 4a and FN = 104 for M. cottoides. A female of M. cottoides with 45m + 21sm + 9st + 6a (2n = 81) plus two extra small chromosomes was found, indicating a natural triploidy with supernumerary chromosomes. The formation of the polyploid individual seems to have come from a diploid female gamete, due to the presence of a marker chromosome pair partially heterochromatic presents only in females and common to that exemplar. This triploid female showed three chromosomes with nitrate staining (AgNOR), 18S rDNA probe and chromomycin A3 (CMA3) staining. AgNORs were observed on pairs 12 and 23 in P. pulcher and pair 24 in M. cottoides, results that were confirmed with an 18S rDNA probe and CMA3 fluorochrome. These are the first chromosomal data for P. pulcher and provide the first description of natural triploidy with the presence of supernumerary chromosomes in this family and emphasizing well the chromosomal rearrangements diversification between this species.

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Acknowledgments

The authors are grateful to Dra. Lucia Giuliano Caetano and Dr. Alberto Sergio Fenocchio for the review of this manuscript and to MSc Bruno Ambrozio Galindo for the collection of Pseudopimelodus pulcher specimens. This research was supported by a grant from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The researcher received permission from Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA) to collect fish specimens.

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  1. Laboratório de Citogenética Animal, Departamento de Biologia Geral, Centro de Ciências Biológicas (CCB), Universidade Estadual de Londrina, P.O Box 6001, Londrina, Paraná, 86051-970, Brazil

    Juceli Gonzalez Gouveia, Vivian Patrícia Oliveira de Moraes, Larissa Bettin Pires, Renata da Rosa & Ana Lúcia Dias

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  1. Juceli Gonzalez Gouveia
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  2. Vivian Patrícia Oliveira de Moraes
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  3. Larissa Bettin Pires
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  5. Ana Lúcia Dias
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Correspondence to Ana Lúcia Dias.

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Gouveia, J.G., de Moraes, V.P.O., Pires, L.B. et al. Comparative cytogenetics between two species of the family Pseudopimelodidae (Siluriformes): occurrence of natural triploidy and supernumerary chromosomes. Cytotechnology 67, 215–222 (2015). https://doi.org/10.1007/s10616-013-9676-x

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