Skip to main content
SpringerLink
Log in

Antimicrobial and antioxidant activities with acute toxicity, cytotoxicity and mutagenicity of Cystoseira compressa (Esper) Gerloff & Nizamuddin from the coast of Urla (Izmir, Turkey)

  • Original Research
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The aim of the study was to evaluate the biological activities with toxic properties of the methanol, hexane, and chloroform extracts of Cystoseira compressa (Esper) Gerloff & Nizamuddin from the Coast of Urla in the Aegean Sea. The extracts of C. compressa were tested for their antimicrobial and antioxidant activities in this study. Cytotoxic and mutagenic potentials of the extracts were also evaluated using cell culture and mutagenicity assays. Hexane extract was found to have higher total flavonoid and phenolic contents than the other extracts and exerted higher antioxidant activity than other extracts. All extracts exhibited moderate antimicrobial activity against tested microorganisms (minimum inhibitory concentration ranges are 32–256 μg/mL). The results indicated that the extracts had no significant cytotoxic activity against human hepatocellular carcinoma Hep 3B cell line in all treated concentrations (5–50 μg/mL) and did not show mutagenicity in the Ames test. Lethality was not observed among mice treated with oral doses of the extracts. In conclusion, results of investigations indicate that brown alga C. compressa is a natural source of antioxidant. It has moderate antimicrobial activities with no toxicity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

References

  • Abourriche A, Charrouf M, Berrada M, Bennamara A, Chaib N, Francisco C (1999) Antimicrobial activities and cytotoxicity of the brown algae Cystoseira tamariscifolia. Fitoterapia 70:611–614

    Article  Google Scholar 

  • Amico V (1995) Marine brown algae family Cystoseiraceae: chemistry and taxonomy. Phytochemistry 39:1257–1279

    Article  CAS  Google Scholar 

  • Amico V, Neri P, Piattelli M, Ruberto G (1987) Geranylgeraniol-derived diterpenes from the brown alga Bifurcaria Bifurcata: comparison with two other Cystoseiraceae species. Phytochemistry 26:2637–2639

    Article  CAS  Google Scholar 

  • Amico V, Cunsolo F, Neri P, Piatelli M, Ruberto G (1988) Antimicrobial tetraprenyl toluquinol derivatives from Cystoseira spinosa var. squarrosa. Phytochemistry 27:1327–1331

    Article  CAS  Google Scholar 

  • Armstrong E, Boyd KG, Burgess JG (2000) Prevention of marine biofouling using natural compounds from marine organisms. Biotechnol Annu Rev 6:221–241

    Article  CAS  Google Scholar 

  • Arumagam P, Ramamurthy P, Santhiya ST, Ramesh A (2006) Antioxidant activity measured in different solvent fractions obtained from Mentha spicata Linn.: an analysis by ABTS+ decolorization assay. Asia Pac J Clin Nutr 15:119–124

    Google Scholar 

  • Ayyad SN, Abdel-Halim OB, Shier WT, Hoye TR (2003) Cytotoxic hydroazulen diterpenes from the brown alga Cystoseira myrica. Z Natureforsch 58:33–38

    CAS  Google Scholar 

  • Ballesteros E, Martin D, Uriz MJ (1992) Biological activity of extracts from some Mediterranean macrophytes. Bot Mar 35:481–485

    Google Scholar 

  • Barile FA (2008) Principles of toxicology testing. CRC Press, New York

    Google Scholar 

  • Bennamara A, Abourriche A, Berrada M, Charrouf M, Chaib N, Boudouma M, Garneau FX (1999) Methoxybifurcarenone: an antifungal and antibacterial meroditerpenoid from the brown alga Cystoseira tamariscifolia. Phytochemistry 52:37–40

    Article  CAS  Google Scholar 

  • Bhosale SH, Nagle VL, Jagtap TG (2002) Antifouling potential of some marine organisms from India species of Bacillus and Pseudomonas. Mar Biotechnol 4:111–118

    Article  CAS  Google Scholar 

  • Caccamese S, Toscano RM, Furnari G, Cormaci M (1985) Antimicrobial activities of red and brown algae from Southern Italy coast. Bot Mar 28:505–507

    Google Scholar 

  • Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182

    CAS  Google Scholar 

  • Culioli G, Ortalo-Magne A, Daoudi M, Thomas-Guuyon H, Valls R, Piovetti L (2004) Trihydroxylated linear diterpenes from the brown alga Bifurcaria bifurcate. Phytochem 65:2063–2069

    Article  CAS  Google Scholar 

  • Derelanko MJ, Hollinger MA (eds) (1995) CRC handbook of toxicology, 2nd edn. CRC Press, Boca Raton, FL

    Google Scholar 

  • Ely R, Supriya T, Naik CG (2004) Antimicrobial activity of marine organisms collected off the coast of South East India. J Exp Biol Ecol 309:121–127

    Article  Google Scholar 

  • Faulkner DJ (1986) Marine natural products. Nat Prod Rep 3:1–33

    Article  CAS  Google Scholar 

  • Faulkner DJ (1993) Marine natural products chemistry: introduction. Chem Rev 93:1671–1673

    Google Scholar 

  • Febles CI, Arias A, Gil-Rodriguez MC, Hardisson A, Sierra Lopez A (1995) In vitro study of antimicrobial activity in algae (Chlorophyta, Phaeophyta and Rhodophyta) collected from the coast of Tenerife (in Spanish). Anuario del Instituto de Estudios Canarios 34:181–192

    Google Scholar 

  • Fenical W, Paul VJ (1984) Antimicrobial and cytotoxic terpenoids from tropical green algae of the family Udoteaceae. Hydrobiologia 116:135–140

    Article  Google Scholar 

  • Gonzalez del Val A, Platas G, Basilio A (2001) Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary Islands, Spain). Int Microbiol 4:35–40

    CAS  Google Scholar 

  • Heiba HI, Al-Nagdy SA, Rizk AM, Durgham MM (1993) The aminoacid composition of some common marine algae from Qatar (Arabian Gulf). Qatar Uni Sci I 13:219–225

    Google Scholar 

  • Hodgson LM (1984) Antimicrobial and antineoplastic activity in some South Florida seaweeds. Bot Mar 27:387–390

    Article  Google Scholar 

  • Hsu HF, Houng JY, Chang CL, Wu CC, Chang FR, Wu YC (2005) Antioxidant activity, cytotoxicity, and DNA information of Glossogyne tenuifolia. J Agric Food Chem 53:6117–6125

    Article  Google Scholar 

  • Huang D, Ou B, Prior L (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem 53:1841–1856

    Article  CAS  Google Scholar 

  • Huve E (1972) Aperçu sur la distribution en mer Égée de quelques espèces du genre Cystoseira (Pheophycees, Fucales). Soc Phycol de France 17:22–37

    Google Scholar 

  • Liu L, Heinrich M, Myers S, Dworjanyn SA (2012) Towards a better understanding of medicinal uses of the brown seaweed Sargassum in Traditional Chinese Medicine: a phytochemical and pharmacological review. J Ethnopharmacol 142:591–619

    Article  Google Scholar 

  • Maron DM, Ames BN (1983) Revised methods for the Salmonella mutagenicity test. Mutat Res 113:173–215

    Article  CAS  Google Scholar 

  • Martí R, Uriz MJ, Ballesteros E, Turon X (2004) Benthic assemblages in two Mediterranean caves: species diversity and coverage as a function of abiotic parameters and geographic distance. J Mar Biol Assoc UK 84:557–572

    Article  Google Scholar 

  • Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG (2005) Determination of the total phenolic, flavonoid and praline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 91:571–577

    Article  CAS  Google Scholar 

  • Mohamed S, Hashim SN, Rahman HA (2012) Seaweeds: a sustainable functional food for complementary and alternative therapy. Trends Food Sci Tech 23:83–96

    Article  CAS  Google Scholar 

  • Mossman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immun Meth 65:55–63

    Article  Google Scholar 

  • NCCLS, National Committee for Clinical Laboratory Standards (2003) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. NCCLS document M7-A6, 8th edn. NCCLS, Wayne, PA

    Google Scholar 

  • Okada Y, Okada M (1998) Scavenging effect of water soluble proteins in broad beans on free radicals and active oxygen species. J Agric Food Chem 46:401–406

    Article  CAS  Google Scholar 

  • Ozdemir G, Horzum Z, Sukatar A, Karabay-Yavasoglu NU (2006) Antimicrobial activities of volatile components and various extracts of Dictyopteris membranaceae and Cystoseira barbata from the coast of Izmir, Turkey. Pharm Biol 44:183–188

    Article  CAS  Google Scholar 

  • Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269:337–341

    Article  CAS  Google Scholar 

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26:1231–1237

    Article  CAS  Google Scholar 

  • Reichelt JL, Borowitzka MA (1984) Antimicrobial activity from marine algae: results of a large-scale screening program. Hydrobiologia 116:158–167

    Article  Google Scholar 

  • Safer AM, al-Nughamish AJ (1999) Hepatotoxicity induced by the antioxidant food additive, butylated hydroxytoluene (BHT), in rats: an electron microscopical study. Histol Histopathol 14:391–406

    CAS  Google Scholar 

  • Salvador N, Gómez-Garreta A, Lavelli L, Ribera MA (2007) Antimicrobial activity of Iberian macroalgae. Sci Mar 71:101–113

    Article  Google Scholar 

  • Sastry VMVS, Rao GRK (1994) Antibacterial substances from marine algae: successive extraction using benzene, chloroform and methanol. Bot Mar 37:357–360

    Article  CAS  Google Scholar 

  • Scheuer PJ (1990) Some marine ecological phenomena: chemical basis and biomedical potential. Science 248:173–177

    Article  CAS  Google Scholar 

  • Seeram NP, Adams LS, Hardy ML, Heber D (2004) Total cranberry extract versus its phytochemical constituents: antiproliferative and synergistic effects against human tumor cell lines. J Agric Food Chem 52:2512–2517

    Article  CAS  Google Scholar 

  • Shao ZH, Vanden Hoek TL, Li CQ, Schumacker PT, Becker LB, Chan KC, Qin Y, Yin JJ, Yuan CS (2004) Synergistic effect of Scutellaria baicalensis and grape seed proanthocyanidins on scavenging reactive oxygen species in vitro. Am J Chin Med 32:89–95

    Article  CAS  Google Scholar 

  • Snedecor GW, Cochran WG (1968) Statistical methods. Oxford and IBD Publishing Co., Calcutta

    Google Scholar 

  • Surakka A, Sihvonen LM, Lehtimäki JM, Wahlsten M, Vuorela P, Sivonen K (2005) Benthic cyanobacteria from the Baltic Sea contain cytotoxic Anabaena, Nodularia and Nostoc strains and an apoptosis inducing Phormidium strain. Environ Toxicol 20:285–292

    Article  CAS  Google Scholar 

  • Takamatsu S, Hodges TW, Rajbhandari I, Gerwick WH, Hamann MT, Nagle DG (2003) Marine natural products as novel antioxidant prototypes. J Nat Prod 66:605–608

    Article  CAS  Google Scholar 

  • Tierney, Croft AK, Hayes M (2010) A review of antihypertensive and antioxidant activities in macroalgae. Bot Mar 53:387–408

    Article  CAS  Google Scholar 

  • Valls R, Piovetti L, Banaigs B, Archavlis A, Pellegrini M (1995) (S)-13-Hydroxygeranylgeraniol-derived furanoditerpenes from Bifurcaria bifurcata. Phytochemistry 39:145–149

    Article  CAS  Google Scholar 

  • Wada K, Nakamura K, Tamai Y, Tsuji M, Sahashi Y, Watanabe K, Ohtsuchi S, Yamamoto K, Ando K, Nagata C (2011) Seaweed intake and blood pressure levels in healthy pre-school Japanese children. Nutr J 10:83

    Article  CAS  Google Scholar 

  • Zimmermann M (1983) Ethical guidelines for investigations of experimental pain in conscious animals. Pain 16:109–110

    Article  CAS  Google Scholar 

  • Zubia M, Robledo D, Freile-Pelegrin Y (2007) Antioxidant activities in tropical marine macroalgae from the Yucatan Peninsula, Mexico. J Appl Phycol 19:449–458

    Article  Google Scholar 

Download references

Acknowledgments

We thank Assistant Prof. Dr. İlker Medine for his help in providing of Hep 3B cells.

Conflict of interest

The authors have declared that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Science, Ege University, 35100, Bornova, Izmir, Turkey

    Adem Güner, Atakan Sukatar & N. Ülkü Karabay Yavaşoğlu

  2. Center for Drug Research and Development and Pharmacokinetic Applications, Ege University, 35100, Bornova, Izmir, Turkey

    Çinel Köksal

  3. Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Turkey

    Şüra Baykan Erel

  4. Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Turkey

    Hüsniye Kayalar

  5. Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey

    Ayşe Nalbantsoy

Authors
  1. Adem Güner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Çinel Köksal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Şüra Baykan Erel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hüsniye Kayalar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ayşe Nalbantsoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Atakan Sukatar
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. N. Ülkü Karabay Yavaşoğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. Ülkü Karabay Yavaşoğlu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Güner, A., Köksal, Ç., Erel, Ş.B. et al. Antimicrobial and antioxidant activities with acute toxicity, cytotoxicity and mutagenicity of Cystoseira compressa (Esper) Gerloff & Nizamuddin from the coast of Urla (Izmir, Turkey). Cytotechnology 67, 135–143 (2015). https://doi.org/10.1007/s10616-013-9668-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-013-9668-x

Keywords

Search

Navigation