Abstract
Mesenchymal stem cells (MSCs) are adult multipotent cells currently employed in several clinical trials due to their immunomodulating, angiogenic and repairing features. The adipose tissue is certainly considered an eligible source of MSCs. Recently, putative adipose tissue derived MSCs (ADMSCs) have been isolated from the mediastinal depots. However, very little is known about the properties, the function and the potential of human mediastinal ADMSCs (hmADMSCs). However, the lack of standardized methodologies to culture ADMSCs prevents comparison across. Herein for the first time, we report a detailed step by step description to optimize the isolation and the expansion methodology of hmADMSCs using a virally inactivated good manufacturing practice (GMP)-grade platelet lysate, highlighting the critical aspects of the procedure and providing useful troubleshooting suggestions. Our approach offers a reproducible system which could provide standardization across laboratories. Moreover, our system is time and cost effective, and it can provide a reproducible source of adipose stem cells to enable future studies to unravel new insights regard this promising stem cell population.
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Acknowledgments
We dedicate this manuscript to Maria Pia Docimo. We also acknowledge Fondazione Roma. We thank, Colin Murdoch, Rosa Puca and Isotta Chimenti for their help and support. This study was supported by the grant University of Rome “Sapienza”, Ateneo 2011 prot. C26A11J528.
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Giacomo Frati and Elena De Falco have contributed equally to this work.
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Siciliano, C., Ibrahim, M., Scafetta, G. et al. Optimization of the isolation and expansion method of human mediastinal–adipose tissue derived mesenchymal stem cells with virally inactivated GMP-grade platelet lysate. Cytotechnology 67, 165–174 (2015). https://doi.org/10.1007/s10616-013-9667-y
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DOI: https://doi.org/10.1007/s10616-013-9667-y