Skip to main content
SpringerLink
Log in

The role of adipose-derived stromal cells and hydroxypropylmethylcellulose in engineering cartilage tissue in vivo

  • Original Research
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with transforming growth factor-β1 (TGF-β1) and basic fibroblast growth factor (bFGF). Histological, immunohistochemistry and RT-PCR analysis confirmed that the ADSCs differentiated into chondrocytes following induction. Induced ADSCs mixed with 15 % HPMC were injected into the subcutaneous tissue of nude mice and, after a period of 8 weeks, newly formed cartilage was observed at the site of injection. The ability of ADSCs cultured in the induction medium with TGF-β1 and bFGF to differentiate into chondrocytes and construct new cartilage indicates that ADSCs are suitable for use as seed cells in cartilage tissue engineering. HPMC, according to its good water solubility and being able to transform from liquid to solid at body temperature, was found to be an ideal scaffold for tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Abdanipour A, Tiraihi T, Delshad A (2011) Trans-differentiation of the adipose tissue-derived stem cells into neuron-like cells expressing neurotrophins by selegiline. Iran Biomed J 15:113–121

    CAS  Google Scholar 

  • Chao PH, Grayson W, Vunjak-Novakovic G (2007) Engineering cartilage and bone using human mesenchymal stem cells. J Orthop Sci 12:398–404

    Article  Google Scholar 

  • Chung C, Burdick JA (2008) Engineering cartilage tissue. Adv Drug Deliv Rev 60:243–262

    Article  CAS  Google Scholar 

  • Cuevas P, Burgos J, Baird A (1988) Basic fibroblast growth factor (FGF) promotes cartilage repair in vivo. Biochem Biophys Res Commun 156:611–618

    Article  CAS  Google Scholar 

  • Du Y, Roh DS, Funderburgh ML, Mann MM, Marra KG, Rubin JP, Li X, Funderburgh JL (2010) Adipose-derived stem cells differentiate to keratocytes in vitro. Mol Vis 16:2680–2689

    CAS  Google Scholar 

  • Edwards DR, Murphy G, Reynolds JJ, Whitham SE, Docherty AJ, Angel P, Heath JK (1987) Transforming growth factor beta modulates the expression of collagenase and metalloproteinase inhibitor. EMBO J 6:1899–1904

    CAS  Google Scholar 

  • Erickson GR, Gimble JM, Franklin DM, Rice HE, Awad H, Guilak F (2002) Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem Biophys Res Commun 290:763–769

    Article  CAS  Google Scholar 

  • Fang B, Song Y, Lin Q, Zhang Y, Cao Y, Zhao RC, Ma Y (2007) Human adipose tissue-derived mesenchymal stromal cells as salvage therapy for treatment of severe refractory acute graft-vs.-host disease in two children. Pediatr Transpl 11:814–817

    Article  CAS  Google Scholar 

  • Fatimi A, Tassin JF, Quillard S, Axelos MA, Weiss P (2008) The rheological properties of silated hydroxypropylmethylcellulose tissue engineering matrices. Biomaterials 29:533–543

    Article  CAS  Google Scholar 

  • Fortier LA, Mohammed HO, Lust G, Nixon AJ (2002) Insulin-like growth factor-I enhances cell-based repair of articular cartilage. J Bone Joint Surg Br 84:276–288

    Article  CAS  Google Scholar 

  • Freed LE, Marquis JC, Nohria A, Emmanual J, Mikos AG, Langer R (1993) Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res 27:11–23

    Article  CAS  Google Scholar 

  • Grimsrud CD, Romano PR, D’Souza M, Puzas JE, Reynolds PR, Rosier RN, O’Keefe RJ (1999) BMP-6 is an autocrine stimulator of chondrocyte differentiation. J Bone Miner Res 14:475–482

    Article  CAS  Google Scholar 

  • Lazarous DF, Shou M, Unger EF (1992) Combined bromodeoxyuridine immunohistochemistry and Masson trichrome staining: facilitated detection of cell proliferation in viable vs. infarcted myocardium. Biotech Histochem 67:253–255

    Google Scholar 

  • Lendeckel S, Jodicke A, Christophis P, Heidinger K, Wolff J, Fraser JK, Hedrick MH, Berthold L, Howaldt HP (2004) Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: case report. J Craniomaxillofac Surg 32:370–373

    Article  Google Scholar 

  • Maltese A, Borzacchiello A, Mayol L, Bucolo C, Maugeri F, Nicolais L, Ambrosio L (2006) Novel polysaccharides-based viscoelastic formulations for ophthalmic surgery: rheological characterization. Biomaterials 27:5134–5142

    Article  CAS  Google Scholar 

  • Mathieu E, Lamirault G, Toquet C, Lhommet P, Rederstorff E, Sourice S, Biteau K, Hulin P, Forest V, Weiss P, Guicheux J, Lemarchand P (2012) Intramyocardial delivery of mesenchymal stem cell-seeded hydrogel preserves cardiac function and attenuates ventricular remodeling after myocardial infarction. PLoS ONE 7:e51991

    Article  CAS  Google Scholar 

  • Merceron C, Portron S, Masson M, Lesoeur J, Fellah BH, Gauthier O, Geffroy O, Weiss P, Guicheux J, Vinatier C (2011) The effect of two- and three-dimensional cell culture on the chondrogenic potential of human adipose-derived mesenchymal stem cells after subcutaneous transplantation with an injectable hydrogel. Cell Transpl 20:1575–1588

    Article  Google Scholar 

  • Ochi M (2004) Clinical results of transplantation of tissue-engineered cartilage and future direction of cartilage repair—novel approach with minimally invasive procedure. Yonsei Med J 45 (suppl):S74–A76

    Google Scholar 

  • Oesser S, Seifert J (2003) Stimulation of type II collagen biosynthesis and secretion in bovine chondrocytes cultured with degraded collagen. Cell Tissue Res 311:393–399

    CAS  Google Scholar 

  • Peterson B, Zhang J, Iglesias R, Kabo M, Hedrick M, Benhaim P, Lieberman JR (2005) Healing of critically sized femoral defects, using genetically modified mesenchymal stem cells from human adipose tissue. Tissue Eng 11:120–129

    Article  CAS  Google Scholar 

  • Picker KM (2003) The 3-D model: comparison of parameters obtained from and by simulating different tableting machines. AAPS PharmSciTech 4:E35

    Google Scholar 

  • Ranera B, Lyahyai J, Romero A, Vazquez FJ, Remacha AR, Bernal ML, Zaragoza P, Rodellar C, Martin-Burriel I (2011) Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue. Vet Immunol Immunopathol 144:147–154

    Article  CAS  Google Scholar 

  • Rodriguez AM, Elabd C, Delteil F, Astier J, Vernochet C, Saint-Marc P, Guesnet J, Guezennec A, Amri EZ, Dani C, Ailhaud G (2004) Adipocyte differentiation of multipotent cells established from human adipose tissue. Biochem Biophys Res Commun 315:255–263

    Article  CAS  Google Scholar 

  • Sellers RS, Zhang R, Glasson SS, Kim HD, Peluso D, D’Augusta DA, Beckwith K, Morris EA (2000) Repair of articular cartilage defects one year after treatment with recombinant human bone morphogenetic protein-2 (rhBMP-2). J Bone Joint Surg Am 82:151–160

    Article  CAS  Google Scholar 

  • Trojani C, Weiss P, Michiels JF, Vinatier C, Guicheux J, Daculsi G, Gaudray P, Carle GF, Rochet N (2005) Three-dimensional culture and differentiation of human osteogenic cells in an injectable hydroxypropylmethylcellulose hydrogel. Biomaterials 26:5509–5517

    Article  CAS  Google Scholar 

  • Vacanti JP (1988) Beyond transplantation. Third annual Samuel Jason Mixter lecture. Arch Surg 123:545–549

    Article  CAS  Google Scholar 

  • Vinatier C, Magne D, Moreau A, Gauthier O, Malard O, Vignes-Colombeix C, Daculsi G, Weiss P, Guicheux J (2007) Engineering cartilage with human nasal chondrocytes and a silanized hydroxypropyl methylcellulose hydrogel. J Biomed Mater Res A 80:66–74

    Article  CAS  Google Scholar 

  • Watabe T, Miyazono K (2009) Roles of TGF-beta family signaling in stem cell renewal and differentiation. Cell Res 19:103–115

    Article  CAS  Google Scholar 

  • Weiss P, Gauthier O, Bouler JM, Grimandi G, Daculsi G (1999) Injectable bone substitute using a hydrophilic polymer. Bone 25(2 Suppl):67S–70S

    Article  CAS  Google Scholar 

  • Weiss S, Hennig T, Bock R, Steck E, Richter W (2010) Impact of growth factors and PTHrP on early and late chondrogenic differentiation of human mesenchymal stem cells. J Cell Physiol 223:84–93

    CAS  Google Scholar 

  • Zhang B, Yang S, Sun Z, Zhang Y, Xia T, Xu W, Ye S (2011) Human mesenchymal stem cells induced by growth differentiation factor 5: an improved self-assembly tissue engineering method for cartilage repair. Tissue Eng Part C Methods 17:1189–1199

    Article  CAS  Google Scholar 

  • Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of People’s Republic of China (No. 30772261) and from the Science and Technology Foundation of Shaanxi Province of People’s Republic of China (No. 2012SF2-01-1).

Author information

Authors and Affiliations

  1. Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, No.17 ChangLe West Street, Xi’an, 710032, ShaanXi, People’s Republic of China

    YuQiao Xu, Yu Han, YuanYuan Liang, DaQing Zhao & JianHua Qiu

  2. State Key Laboratory of Cancer Biology and Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China

    YuQiao Xu, Yu Ma, Jie Min & DaQing Zhao

  3. Department of Nephrology and Endocrinology, No. 371 Central Hospital of People’s Liberation Army, Xinxiang, Henan, People’s Republic of China

    Jing Zhang

Authors
  1. YuQiao Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jie Min
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. YuanYuan Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. DaQing Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. JianHua Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to DaQing Zhao or JianHua Qiu.

Additional information

YuQiao Xu and Jing Zhang have contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, Y., Zhang, J., Ma, Y. et al. The role of adipose-derived stromal cells and hydroxypropylmethylcellulose in engineering cartilage tissue in vivo. Cytotechnology 66, 779–790 (2014). https://doi.org/10.1007/s10616-013-9627-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-013-9627-6

Keywords

Search

Navigation