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Impact of stress hormone on adipogenesis in the 3T3-L1 adipocytes

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Abstract

Stress hormone is known to play a vital role in lipolysis and adipogenesis in fat cells. The present study was carried out to evaluate the effect of epinephrine on adipogenesis in the 3T3-L1 cells. The investigation on adipogenesis was done in both mono and co-cultured 3T3-L1 cells. 3T3-L1 preadipocytes and C2C12 cells were grown independently on transwell plates and transferred to differentiation medium. Following differentiation, C2C12 cells transferred to 3T3-L1 plate and treated with medium containing 10 μg/ml of epinephrine. Adipogenic markers such as fatty acid binding protein 4, peroxisome proliferator activating receptor, CCAAT/enhancer-binding protein, adiponectin, lipoprotein lipase and fatty acid synthase mRNA expressions were evaluated in the 3T3-L1 cells. Epinephrine treatment reduced adipogenesis, evidenced by reducing adipogenic marker mRNA expression in the 3T3-L1 cells. In addition, glycerol accumulation and oil red-O staining supported the reduced rate of adipogenesis. Taking all together, it is concluded that the stress hormone, epinephrine reduces the rate of adipogenesis in the mono and co-cultured 3T3-L1 cells. In addition, the rate of adipogenesis is much reduced in the co-cultured 3T3-L1 cells compared monocultured 3T3-L1 cells.

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Acknowledgments

This work was supported by Catholic University of Daegu, South Korea.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Catholic University of Daegu, Daegu, South Korea

    Muthuraman Pandurangan

  2. Department of Pharmaceutical Science and Technology, Catholic University of Daegu, Daegu, South Korea

    Sambandam Ravikumar

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  1. Muthuraman Pandurangan
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  2. Sambandam Ravikumar
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Correspondence to Muthuraman Pandurangan.

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Pandurangan, M., Ravikumar, S. Impact of stress hormone on adipogenesis in the 3T3-L1 adipocytes. Cytotechnology 66, 619–624 (2014). https://doi.org/10.1007/s10616-013-9614-y

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  • DOI: https://doi.org/10.1007/s10616-013-9614-y

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