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Cytotechnology

, Volume 66, Issue 3, pp 431–441 | Cite as

Gastroprotective, cytoprotective and antioxidant effects of Oleum cinnamomi on ethanol induced damage

  • Cansu Ozbayer
  • Hulyam Kurt
  • Zeynep Ozdemir
  • Tunc Tuncel
  • Selva Moheb Saadat
  • Dilek Burukoglu
  • Hakan Senturk
  • Irfan Degirmenci
  • Hasan Veysi Gunes
Original Research

Abstract

Peptic ulcer disease is a gastrointestinal disorder defined by mucosal damage and free oxygen radicals associated with peptic ulcer and gastritis. Cinnamon is a traditional herb used for many diseases and it has also effects as an antioxidant, anti-inflammatory, antispasmodic and anti-ulcerative. Our research is based on oxidative stress and effects of Oleum cinnamomi on stomach, liver and kidney disorders induced by ethanol. In our experiment, 2–3 month old male Sprague–Dawley rats were used. One hour before the mucosal damage induced by 70 % ethanol, O. cinnamomi (2.5 ml/kg) was added into the groups. Gastric pH, analysis of gastric mucus and ulcer index were calculated from samples obtained from the stomach. Superoxide dismutase (SOD), malondialdehyde and catalase (CAT) levels were determined in stomach, liver and kidney homogenates and erythrocyte hemolysate. Histopathological examination of stomach, liver and kidney were determined with H&E staining. The non-treated ulcerative group showed higher scores than the control group which was treated with O. cinnamomi, when ulcer scores, gastric mucus and pH level of stomach are compared. Increased lipid peroxidation levels were observed in the liver, kidney and erythrocyte hemolysate. SOD activity was decreased in liver whereas increased in stomach of ethanol treated ulcerative groups. CAT levels were increased in stomach and liver of ethanol treated rats. Histopathological findings showed that ethanol treatment cause multiply organ damage such as stomach, liver and kidney injury. O. cinnamomi treatment protected these tissues from ethanol-induced damage. Consequently, the current investigation shows that O. cinnamomi has protective effects on ethanol-induced oxidative and mucosal damage.

Keywords

Oleum cinnamomi Ethanol Oxidative stress Rat Ulcer Ulcer index 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Cansu Ozbayer
    • 1
  • Hulyam Kurt
    • 1
  • Zeynep Ozdemir
    • 1
  • Tunc Tuncel
    • 1
  • Selva Moheb Saadat
    • 1
  • Dilek Burukoglu
    • 2
  • Hakan Senturk
    • 3
  • Irfan Degirmenci
    • 1
  • Hasan Veysi Gunes
    • 1
  1. 1.Department of Medical Biology, Medical FacultyEskisehir Osmangazi UniversityEskisehirTurkey
  2. 2.Department of Histology, Medical FacultyEskisehir Osmangazi UniversityEskisehirTurkey
  3. 3.Department of Biology, Faculty of Arts and SciencesEskisehir Osmangazi UniversityEskisehirTurkey

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