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Functional analysis of a survivin-like gene in Bombyx mori

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Abstract

The survivin (svv) gene is a newly discovered member of the inhibitors of apoptosis gene family. In recent years, svv has been confirmed to have an anti-apoptosis function and to play a critical role in cell division. We identified a survivin-like gene in the silkworm, Bombyx mori (Bm-svv). In this study, to gain insight into its function, a baculovirus expression system was used to express the Bm-svv gene in insect cell lines. The recombinant viruses were then used as a vector to transform insect cells, and cell activity was determined using the Cell Counting Kit-8 (CCK-8), which is usually employed for detecting mammalian cell number. The results indicated that the Bm-svv gene plays a role in the cell growth arrest or apoptosis induced by viruses. Furthermore, the CCK-8 kit is effective in determining the activity of insect cells.

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Acknowledgments

We thank Professor Jiang Zhu from Soochow University for providing the Spli cells.

Conflict of interest

There is no conflict of interest in this paper.

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Authors and Affiliations

  1. Institute of Life Sciences, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China

    Hui Tang, Yu Wu, Yan Wu, Huiling Wu & Wenbing Wang

  2. Biomedical Development Center of Acebright Group, 1999 Zhangheng Rd, Shanghai, 201203, China

    Haixiang Wu

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  1. Hui Tang
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  2. Yu Wu
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  3. Haixiang Wu
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  4. Yan Wu
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  5. Huiling Wu
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Correspondence to Wenbing Wang.

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Tang, H., Wu, Y., Wu, H. et al. Functional analysis of a survivin-like gene in Bombyx mori. Cytotechnology 66, 181–191 (2014). https://doi.org/10.1007/s10616-013-9551-9

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