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Monitoring of the effects of transfection with baculovirus on Sf9 cell line and expression of human dipeptidyl peptidase IV

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Abstract

Human dipeptidylpeptidase IV (hDPPIV) is an enzyme that is in hydrolase class and has various roles in different parts of human body. Its deficiency may cause some disorders in the gastrointestinal, neurologic, endocrinological and immunological systems of humans. In the present study, hDPPIV enzyme was expressed on Spodoptera frugiperda (Sf9) cell lines as a host cell, and the expression of hDPPIV was obtained by a baculoviral expression system. The enzyme production, optimum multiplicity of infection, optimum transfection time, infected and uninfected cell size and cell behavior during transfection were also determined. For maximum hDPPIV (269 mU mL−1) enzyme, optimum multiplicity of infection (MOI) and time were 0.1 and 72 h, respectively. The size of infected cells increased significantly (P < 0.001) after 24 h post infection. The results indicated that Sf9 cell line was applicable to the large scale for hDPPIV expression by using optimized parameters (infection time and MOI) because of its high productivity (4.03 mU m L−1 h−1).

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Acknowledgments

This research was partly supported by grant from Japan Student Service Organization (JASSO).

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Authors and Affiliations

  1. Department of Food Engineering, Faculty of Engineering, Pamukkale University, Kinikli, Denizli, Turkey

    Özlem Üstün-Aytekin

  2. Bioengineering Department, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey

    Özlem Üstün-Aytekin, İsmet Deliloğlu Gürhan & Gaye Öngen

  3. Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan

    Özlem Üstün-Aytekin, Kayoko Ohura & Teruko Imai

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  1. Özlem Üstün-Aytekin
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  2. İsmet Deliloğlu Gürhan
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Correspondence to Özlem Üstün-Aytekin.

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Üstün-Aytekin, Ö., Gürhan, İ.D., Ohura, K. et al. Monitoring of the effects of transfection with baculovirus on Sf9 cell line and expression of human dipeptidyl peptidase IV. Cytotechnology 66, 159–168 (2014). https://doi.org/10.1007/s10616-013-9549-3

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