, Volume 65, Issue 5, pp 795–802 | Cite as

Optimized flow cytometry protocol for analysis of surface expression of interleukin-1 receptor types I and II

  • Filipp Filippovich Vasilyev
  • Julia Anatolievna Lopatnikova
  • Sergey Vitalievich SennikovEmail author
Method in Cell Science


The biological effects of interleukin (IL)-1 are realized through binding to specific membrane-bound receptors. The efficiency of IL-1 action depends on the number of receptors on the cell. We determined the percentage of cells that express IL-1 receptor type I (IL-1RI) and IL-1 receptor type II (IL-1RII) by flow cytometry using phycoerythrin (PE)-labelled antibodies to the IL-1Rs, and the mean absolute number of membrane-bound IL-1Rs per cell using QuantiBRITE PE calibration beads. We showed that different subpopulations of immunocompetent cells expressed different numbers of molecules of membrane-bound IL-1RI and IL-1RII. We also established that when cells were stimulated with bacterial lipopolysaccharide, there was a significant increase in the number of IL-1RI expressed, and a significant decrease in the mean number of IL-1RII molecules per cell. Determination of the mean number of membrane-bound IL-1R molecules using this protocol enables us to obtain precise and reproducible data that are necessary for full evaluation of expression levels.


Flow cytometry Interleukin-1 receptor Quantitative analysis Peripheral blood mononuclear cells 



This study was supported by the Federal Target Program “Scientific and scientific-pedagogical personnel of innovative Russia” for 2009–2013 (state contract no. 02.740.11.0707).


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Filipp Filippovich Vasilyev
    • 1
  • Julia Anatolievna Lopatnikova
    • 1
  • Sergey Vitalievich Sennikov
    • 1
    Email author
  1. 1.Laboratory of Molecular Immunology, Federal State Budgetary Institution “Research Institute of Clinical Immunology”Russian Academy of Medical Sciences Siberian BranchNovosibirskRussia

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