, Volume 66, Issue 1, pp 119–131 | Cite as

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

  • Nanding Wang
  • Huan Wang
  • Jun Chen
  • Xiaofeng Zhang
  • Juan Xie
  • Zhi Li
  • Jing Ma
  • Wen Wang
  • Zongren Wang
Original Research


Multi-differentiation capability is an essential characteristic of bone marrow mesenchymal stem cells (BMSCs). Method on obtaining higher-quality stem cells with an improved differentiation potential has gained significant attention for the treatment of clinical diseases and developmental biology. In our study, we investigated the multipotential differentiation capacity of BMSCs under simulated microgravity (SMG) condition. F-actin staining found that cytoskeleton took on a time-dependent change under SMG condition, which caused spindle to round morphological change of the cultured cells. Quantitative PCR and Western Blotting showed the pluripotency marker OCT4 was up-regulated in the SMG condition especially after SMG of 72 h, which we observed would be the most appropriate SMG duration for enhancing pluripotency of BMSCs. After dividing BMSCs into normal gravity (NG) group and SMG group, we induced them respectively in endothelium oriented, adipogenic and neuronal induction media. Immunostaining and Western Blotting found that endothelium oriented differentiated BMSCs expressed higher VWF and CD31 in the SMG group than in the NG group. The neuron-like cells derived from BMSCs in the SMG group also expressed higher level of MAP2 and NF-H. Furthermore, the quantity of induced adipocytes increased in the SMG group compared to the NG group shown by Oil Red O staining, The expression of PPARγ2 increased significantly under SMG condition. Therefore, we demonstrated that SMG could promote BMSCs to differentiate into many kinds of cells and predicted that enhanced multi-potential differentiation capacity response in BMSCs following SMG might be relevant to the changes of cytoskeleton and the stem cell marker OCT4.


Simulated microgravity Bone marrow mesenchymal stem cells Pluripotency Differentiation OCT4 



Simulated microgravity


Normal gravity


Mesenchymal stem cell


Bone marrow mesenchymal stem cell


Von Willebrand factor


Microtubule-associated protein 2


Neurofilament heavy chain


Octamer-binding transcription factor 4



This work was carried out in the Physiology laboratory and State Key Laboratory of Aerospace Biodynamics at Fourth Military Medical University. The project was supported by NSFC Grant 30973808.

Supplementary material

10616_2013_9544_MOESM1_ESM.tif (16.6 mb)
Supplementary material 1 (TIFF 16991 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Nanding Wang
    • 1
  • Huan Wang
    • 2
  • Jun Chen
    • 3
  • Xiaofeng Zhang
    • 1
  • Juan Xie
    • 1
  • Zhi Li
    • 4
  • Jing Ma
    • 1
  • Wen Wang
    • 1
  • Zongren Wang
    • 1
  1. 1.Department of Traditional Chinese Medicine, Xijing HospitalFourth Military Medical UniversityXi’anPeople’s Republic of China
  2. 2.Department of Dermatology, Tangdu HospitalFourth Military Medical UniversityXi’anPeople’s Republic of China
  3. 3.Department of EncephalopathyTraditional Chinese Medicine Hospital of Shaanxi ProvinceXi’anPeople’s Republic of China
  4. 4.Department of Cardiovascular Surgery, Xijing HospitalFourth Military Medical UniversityXi’anPeople’s Republic of China

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