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Evaluation of the effects of nicorandil and its molecular precursor (without radical NO) on proliferation and apoptosis of 786-cell

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Abstract

Nicorandil is a nitric oxide (NO) donor used in the treatment of angina symptoms. It has also been reported to protect cells and affect the proliferation and death of cells in some tissues. The molecules that interfere with these processes can cause dysfunction in healthy tissues but can also assist in the therapy of some disorders. In this study we examined the effect of nicorandil and of the molecular precursor that does not have the NO radical (N-(beta-hydroxyethyl) nicotinamide) on the cell proliferation and death of human renal carcinoma cells (786-O) under normal oxygenation conditions. The molecular precursor was used in order to analyze the effects independents of NO. In the cytotoxicity test, nicorandil was shown to be cytotoxic at very high concentrations and it was more cytotoxic than its precursor (cytotoxic at concentrations of 2,000 and 3,000 μg/mL, respectively). We propose that the lower cytotoxicity of the precursor is due to the absence of the NO radical. In this study, the cells exposed to nicorandil showed neither statistically significant changes in cell proliferation nor increases in apoptosis or genotoxicity. The precursor generated similar results to those of nicorandil. We conclude that nicorandil causes no changes in the proliferation or apoptosis of the cell 786-O in normal oxygenation conditions. Moreover, the lack of NO radical in the precursor molecule did not show a different result, except in the cell cytotoxicity.

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Acknowledgments

We would like to thank the Coordination of Improvement of Higher Education Personnel (CAPES), National Council for Scientific and Technological Development (CNPq) and Araucaria Foundation for their financial support.

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Authors and Affiliations

  1. Laboratório de Genética Toxicológica, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, Pr 445 Km 380, CEP 86055-990, Londrina, Paraná, Brazil

    Natália Aparecida de Paula, Andressa Megumi Niwa, Diogo Campos Vesenick & Mário Sérgio Mantovani

  2. Laboratório de Imunopatologia Experimental, Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, CEP 86051-990, Londrina, Paraná, Brazil

    Carolina Panis & Rubens Cecchini

  3. Departamento de Química, Universidade Federal de Minas Gerais, CEP 31270-901, Pampulha, Belo Horizonte, Minas Gerais, Brazil

    Ângelo de Fátima

  4. Instituto de Biociências, Universidade Estadual Paulista, CEP 13506-900, Rio Claro, São Paulo, Brazil

    Lúcia Regina Ribeiro

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  1. Natália Aparecida de Paula
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  2. Andressa Megumi Niwa
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  4. Carolina Panis
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  8. Mário Sérgio Mantovani
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Correspondence to Natália Aparecida de Paula.

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de Paula, N.A., Niwa, A.M., Vesenick, D.C. et al. Evaluation of the effects of nicorandil and its molecular precursor (without radical NO) on proliferation and apoptosis of 786-cell. Cytotechnology 65, 839–850 (2013). https://doi.org/10.1007/s10616-012-9524-4

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  • DOI: https://doi.org/10.1007/s10616-012-9524-4

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