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Natural IgG antibody with anti-β-galactosyl specificity suppressed hepatoma cell invasion in culture

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Abstract

The effect of natural IgG antibody recognizing β-galactosyl epitope on hepatoma cell invasion was investigated. Anti-β-galactosyl antibody dose-dependently suppressed hepatoma invasion underneath primarily cultured mesothelial cells monolayer without affecting the proliferation, to the same extent as natural IgG antibody with anti-α-galactosyl specificity, which had already been reported to have an anti-metastatic activity. The inhibitory effect of anti-β-galactosyl antibody was completely canceled by adding lactose (galactose-β-1, 4-glucose) to the medium, indicating that this antibody recognized some antigens with β-galactosyl epitope. Hepatoma cells pretreated with this antibody for 48 h showed reduced invasive activity, while the pretreatment of mesothelial cells with the antibody did not affect hepatoma cells invasion. Anti-β-galactosyl antibody also suppressed hepatoma cells adhesion to mesothelial cells monolayer. These results suggest that natural antibody with anti-β-galactosyl specificity may recognize the β-galactosyl epitope in some adhesion-related molecules on hepatoma cells, thus suppressing adhesion and invasion to mesothelial cells monolayer. These results suggest possible therapeutic uses of this antibody in the treatment of metastatic tumors.

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Author notes

  1. Fumihiko Sakai

    Present address: Milk Science Research Institute, Megmilk Snow Brand Co., Minamidai, Kawagoe, Saitama, 350-1165, Japan

Authors and Affiliations

  1. Department of Applied Biological Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan

    Yutaka Miura, Hiroshi Fujita, Fumihiko Sakai, Kazumi Yagasaki & Daisaburo Fujimoto

  2. Graduate School of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan

    Hiroyuki Tachikawa

Authors
  1. Yutaka Miura
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  2. Hiroshi Fujita
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  3. Fumihiko Sakai
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  4. Hiroyuki Tachikawa
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  5. Kazumi Yagasaki
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  6. Daisaburo Fujimoto
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Correspondence to Yutaka Miura.

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Miura, Y., Fujita, H., Sakai, F. et al. Natural IgG antibody with anti-β-galactosyl specificity suppressed hepatoma cell invasion in culture. Cytotechnology 65, 909–913 (2013). https://doi.org/10.1007/s10616-012-9523-5

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  • DOI: https://doi.org/10.1007/s10616-012-9523-5

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