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Combination of yeast hydrolysates to improve CHO cell growth and IgG production

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Abstract

Many studies underlined the great benefits of hydrolysates used as additives in animal free media on cell culture performances. However, to precisely define hydrolysate supplementation strategies, a deeper understanding of their effect on cell growth and protein production is required. In the present study, the effect of addition of one yeast extract (YE) and two yeast peptones (named YP.A and YP.B) in a chemically defined medium was first assessed on cell culture performances. Interestingly, specific effects were found depending on the degree of degradation of yeast hydrolysates. The YE at 1 g L−1 increased the maximal cell density by 70 %, while a mixture of YE (1 g L−1) and YP.A (4 g L−1) increased IgG production by 180 %. These conditions were then evaluated on the CHO cell kinetics all over cultures. Hydrolysates extended the cell growth phase in Erlenmeyer flask and increased the maximal growth rate in bioreactor up to 20 %. Cell growth stimulation induced by hydrolysates addition was linked with energetic metabolism improvement suggesting that they promote oxidative pathway. Furthermore, hydrolysates provided an additional source of substrate that supported cell growth despite glutamine limitation.

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Acknowledgments

The authors would like to acknowledge Dr. Florian Wurm (EPFL, Lausanne) for the supply of the recombinant CHO cell line.

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Authors and Affiliations

  1. CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505, Vandœuvre-lès-Nancy, France

    Mathilde Mosser, Isabelle Chevalot, Eric Olmos, Fabrice Blanchard, Romain Kapel, Ivan Marc & Annie Marc

  2. ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505, Vandœuvre-lès-Nancy, France

    Mathilde Mosser, Isabelle Chevalot, Eric Olmos, Fabrice Blanchard, Romain Kapel, Ivan Marc & Annie Marc

  3. Bio Springer, 103 rue Jean Jaurès, 94704, Maisons-Alfort, France

    Mathilde Mosser & Eric Oriol

  4. LRGP, UPR-CNRS 3349, Université de Lorraine, 2, avenue de la forêt de Haye, 54505, Vandœuvre-lès-Nancy, France

    Isabelle Chevalot

Authors
  1. Mathilde Mosser
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  2. Isabelle Chevalot
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  3. Eric Olmos
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  4. Fabrice Blanchard
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  5. Romain Kapel
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  6. Eric Oriol
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  7. Ivan Marc
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  8. Annie Marc
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Correspondence to Isabelle Chevalot.

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Mosser, M., Chevalot, I., Olmos, E. et al. Combination of yeast hydrolysates to improve CHO cell growth and IgG production. Cytotechnology 65, 629–641 (2013). https://doi.org/10.1007/s10616-012-9519-1

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  • DOI: https://doi.org/10.1007/s10616-012-9519-1

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