Abstract
Gliomas can diffuse into the normal brain and this invasion of glioma cells involves modification of receptor-mediated adhesive properties of tumor cells, degradation and remodeling of extracellular matrix by tumor-secreted metalloproteinase (MMPs) such as MMP-2, consequently creating an intercellular space for invasion of glioma cells. BmK CT, one of the key toxins in scorpion Buthus martensii Karsch venom, is a novel blocker of the chloride ion channel and MMP-2. In this report, a recombinant plasmid pEGFP-N1-BmK CT was constructed and characterized by in vitro studies. The results showed that pEGFP-N1 mediated BmK CT expression displayed a high activity in suppressing cell migration via MMP-2. The potential therapeutic effect of pEGFP-N1 mediated BmK CT against rat glioma C6 cells was assessed and its potential mechanism was elucidated. It represented an approach for developing a novel therapeutic agent—recombinant plasmid pEGFP-N1-BmK CT as an efficient and powerful adjuvant.
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Acknowledgments
This project was supported by grants from ‘National Natural Science Foundation of China (No.31272100,31071924)’, the ‘National High Technology Research and Development Program of China (863 Program, No.2012AA020809)’, and ‘the Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi’.
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Fu, Y., Jiao, Y., An, N. et al. pEGFP-N1-mediated BmK CT expression suppresses the migration of glioma. Cytotechnology 65, 533–539 (2013). https://doi.org/10.1007/s10616-012-9518-2
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DOI: https://doi.org/10.1007/s10616-012-9518-2