, Volume 65, Issue 3, pp 355–362 | Cite as

Lectin microarrays differentiate carcinoma cells from reactive mesothelial cells in pleural effusions

  • Yu-Qing Shi
  • Qun He
  • Yu-Jie Zhao
  • En-Hua Wang
  • Guang-Ping Wu
Original Research


The aim of this study was to evaluate the diagnostic utility of lectin microarrays in pleural effusions of patients with lung cancer. A lectin microarray, LTL, PSA, LCA, UEA-1, AAL, MAL-I, MAL-II, SNA, WGA, ECL, DSA, STL, SWGA, HPA, ConA, GNA, HHL, BPL, EEL, Jacalin, WFA, ACL, MPL, DBA, SBA, was used to determine the glycoprotein profile of cells in pleural effusions from patients with lung cancer (54 cases), and with benign lung disease (54 cases). The A549 cell line, used as an experimental control, was positive for AAL, MAL-I, WGA, STL, Jacalin and ACL binding. Adenocarcinoma cells in pleural effusions were positive for ECL, DSA, AAL, MAL-I, WGA, STL, Jacalin, and ACL binding. AAL, WGA, and ACL positive binding was the most common, found in 54, 48, and 38 samples, respectively. ECL and DSA binding was positive in only 4 samples. In comparison, reactive mesothelial cells displayed positive binding for all markers in the microarray panel. SNA and AAL positive binding was detected in the majority of samples; 50/54 and 48/54 samples, respectively. Positive binding of DBA, MAL-II and EEL was present in only 2, 4 and 4 samples, respectively. SNA binding had the highest sensitivity (92.6 %), specificity (100 %), and accuracy (96.3 %). SNA may be used as a biomarker to distinguish reactive mesothelial cells from adenocarcinoma cells. The lectin microarrays proved able to distinguish carcinoma cells from reactive mesothelial cells in pleural effusions.


Cytopathology Effusion, pleural Lectin microarray Lung cancer 



This work was supported by grants from the National Natural Science Foundation of China (to Guang-Ping Wu, Grant No.81171650 and to Qun He, Grant No.20672144).


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yu-Qing Shi
    • 1
  • Qun He
    • 2
  • Yu-Jie Zhao
    • 2
  • En-Hua Wang
    • 1
  • Guang-Ping Wu
    • 1
  1. 1.Department of Pathology, The First Affiliated Hospital and College of Basic Medical SciencesChina Medical UniversityShenyangChina
  2. 2.Center of Biochip, College of Basic Medical SciencesChina Medical UniversityShenyangChina

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