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Inhibitory effect of tannins from galls of Carpinus tschonoskii on the degranulation of RBL-2H3 Cells

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Abstract

In this study, the anti-allergy potency of thirteen tannins isolated from the galls on buds of Carpinus tschonoskii (including two tannin derivatives) was investigated. RBL-2H3 (rat basophilic leukemia) cells were incubated with these compounds, and the release of β-hexosaminidase and cytotoxicity were measured. Of the thirteen tannins, tetragalloylglucose (2), pentagalloylglucose (3), casuarictin (4), and casuarinin (9) were the most potent inhibitors, and all the tannins showed no cytotoxic effect after 24 h of incubation. The results obtained suggest that tannins from C. tschonoskii are capable of inhibiting allergic reactions and may be useful for the treatment or prevention of type I allergic diseases.

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References

  • Adams LS, Zhang Y, Seeram NP, Heber D, Chen S (2010) Pomegranate ellagitannin-derived compounds exhibit antiproliferative and antiaromatase activity in breast cancer cells in vitro. Cancer Prev Res 3:108–113

    Article  CAS  Google Scholar 

  • Baumgartner RR, Steinmann D, Heiss EH, Atanasov AG, Ganzera M, Stuppner H, Dirsc VM (2010) Bioactivity-guided isolation of 1,2,3,4,6-Penta-O-galloyl-d-glucopyranose from Paeonia lactiflora roots as a PTP1B inhibitor. J Nat Prod 73:1578–1581

    Article  CAS  Google Scholar 

  • Beaven MA, Metzger H (1993) Signal transduction by Fc receptors: the FcεRI case. Immunol Today 14:222–226

    Article  CAS  Google Scholar 

  • Chen CH, Liu TZ, Kuo TC, Lu FJ, Chen YC, Chang-Chien YW, Lin CC (2004) Casuarinin protects cultured MDCK cells from hydrogen peroxide-induced oxidative stress and DNA oxidative damage. Planta Med 70:1022–1026

    Article  CAS  Google Scholar 

  • Cheng HY, Lin TC, Yu KH, Yang CM, Lin CC (2003) Antioxidant and free radical scavenging activities of Terminalia chebula. Biol Pharm Bull 26:1331–1335

    Article  CAS  Google Scholar 

  • Cheong H, Choi EJ, Yoo GS, Kim KM, Ryu SY (1998) Desacetylmatricarin, an anti-allergic compound from Taraxacum platycarpum. Planta Med 64:577–578

    Article  CAS  Google Scholar 

  • Clifford MN, Scalbert A (2000) Ellagitannins—nature, occurrence and dietary burden. J Sci Food Agric 80:1118–1125

    Article  CAS  Google Scholar 

  • Fukuda T, Ito H, Yoshida T (2003) Antioxidative polyphenols from walnuts (Juglans regia L.). Phytochemistry 63:795–801

    Article  CAS  Google Scholar 

  • Ishimoto H, Shibata M, Myojin Y, Ito H, Sugimoto Y, Tai A, Hatano T (2011) In vivo anti-inflammatory and antioxidant properties of ellagitannin metabolite urolithin A. Bioorg Med Chem Lett 21:5901–5904

    Article  CAS  Google Scholar 

  • Ito H (2011) Metaboloties of the ellagitannin geraniin and their antioxidant activities. Planta Med 77:1110–1115

    Article  CAS  Google Scholar 

  • Kamijo M, Kanazawa T, Funaki M, Nishizawa M, Yamagishi T (2008) Effects of rosa rugosa petals on intestinal bacteria. Biosci Biotechnol Biochem 72:773–777

    Article  CAS  Google Scholar 

  • Kanda T, Akiyama H, Yanagida A, Tanabe M, Goda Y, Toyoda M, Teshima R, Saito Y (1998) Inhibitory effects of apple polyphenol on induced histamine release from RBL-2H3 cells and rat mast cells. Biosci Biotechnol Biochem 62:1284–1289

    Article  CAS  Google Scholar 

  • Kanoh R, Hatano T, Ito H, Yoshida T, Akagi M (2000) Effects of tannins and related polyphenols on superoxide-induced histamine release from rat peritoneal mast cells. Phytomedicine 7:297–302

    Article  CAS  Google Scholar 

  • Kim SH, Jun CD, Suk K, Choi BJ, Lim H, Park SS, Lee H, Shin HY, Kim DK, Shin TY (2006) Gallic acid inhibits histamine release and pro-inflammatory cytokine production in mast cells. Toxicol Sci 91:123–131

    Article  CAS  Google Scholar 

  • Larrosa M, Gonzalez-Sarrias A, Yanez-Gascon MJ, Selma MV, Azorin-Ortuno M, Toti S, Tomas-Barberan F, Dolara P, Espin JC (2010) Anti-inflammatory properties of a pomegranate extract and its metabolite urolithin-A in a colitis rat model and the effect of colon inflammation on phenolic metabolism. J Nutr Biochem 21:717–725

    Article  CAS  Google Scholar 

  • Lee HJ, Seong YH, Bae KH, Kwon SH, Kwak HM, Nho SK, Kim KA, Hur JM, Lee KB, Kang YH, Song KS (2005) Beta-secretase (BACE1) inhibitors from Sanguisorbae Radix. Arch Pharm Res 2:799–803

    Article  Google Scholar 

  • Matsuo N, Yamada K, Shoji K, Mori M, Sugano M (1997) Effect of tea polyphenols on histamine release from rat basophilic leukemia (RBL-2H3) cells: The structure-inhibitory activity relationship. Allergy 52:58–64

    Article  CAS  Google Scholar 

  • Mossman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  Google Scholar 

  • Novak N, Kraft S, Bieber T (2001) IgE receptors. Curr Opin Immunol 13:721–726

    Article  CAS  Google Scholar 

  • Okuda T, Yoshida T, Hatano T (1989) New methods of analyzing tannins. J Nat Prod 52:1–31

    Article  CAS  Google Scholar 

  • Okuda T, Yoshida T, Hatano T, Ito H (2009) Ellagitannins renewed the concept of tannins. In: Quideau S (ed) Chemistry and biology of ellagitannins. World Scientific Publishing Co. Pte. Ltd., Singapore, pp 40–54

    Google Scholar 

  • Ono T, Shigemori H (2009) Two new hydrolyzable tannins, carpinerins A and B, from galls of Carpinus tschonoskii. Heterocycles 78:1993–2001

    Article  CAS  Google Scholar 

  • Ortega E, Schweitzer-Stenner R, Pecht I (1988) Possible orientational constraints determine secretory signals induced by aggregation of IgE receptors on mast cells. EMBO J 7:4101–4109

    CAS  Google Scholar 

  • Pan MH, Lin-Shiau SY, Ho CT, Lin JH, Lin JK (2000) Suppression of lipopo-lysaccharide-induced nuclear factor-kappaB activity by theaflavin-3, 3′-digallate from black tea and other polyphenols through down-regulation of IkappaB kinase activity in macrophages. Biochem Pharmacol 59:357–367

    Article  CAS  Google Scholar 

  • Passante E, Frankish N (2009) The RBL-2H3 cell line: its provenance and suitability as a model for the mast cell. Inflamm Res 58:737–745

    Article  CAS  Google Scholar 

  • Schiwartz LB, Lewis RA, Seldin D, Austen KF (1981) Acid hydrolases and tryptase from secretory granules of dispersed human lung mast cells. J Immunol 126:1290–1294

    Google Scholar 

  • Schroeder JT, Kagey-Sobotka A, Lichtenstein LM (1995) The role of the basophil in allergic inflammation. Allergy 50:463–472

    Article  CAS  Google Scholar 

  • Seeram NP, Lee R, Heber D (2004) Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice. Clin Chim Acta 348:63–68

    Article  CAS  Google Scholar 

  • Shimoda H, Tanaka J, Kikuchi M, Fukuda T, Ito H, Hatano T, Yoshida T (2008) Walnut polyphenols prevent liver damage induced by carbon tetrachloride and D-galactosamine: hepatoprotective hydrolyzable tannins in the kernel pllicles of walnut. J Agric Food Chem 56:4444–4449

    Article  CAS  Google Scholar 

  • Suzuki M, Nakamura T, Iyoki S, Fujiwara A, Watanabe Y, Mohri K, Isobe K, Ono K, Yano S (2005) Elucidation of anti-allergic activities of curcumin-related compounds with a special reference to their anti-oxidative activities. Biol Pharm Bull 28:1438–1443

    Article  CAS  Google Scholar 

  • Tokura T, Nakano N, Ito T, Matsuda H, Nagasako-Akazome Y, Kanda T, Ikeda M, Okumura K, Ogawa H, Nishiyama C (2005) Inhibitory effect of polyphenol-enriched apple extracts on mast cell degranulation in vitro targeting the binding between IgE and Fcε RI. Biosci Biotechnol Biochem 69:1974–1977

    Article  CAS  Google Scholar 

  • Yamada P, Zarrouk M, Kawasaki K, Isoda H (2008) Inhibitory effect of various Tunisian olive oils on chemical mediator release and cytokine production by basophilic cells. J Ethnopharmacol 116:279–287

    Article  CAS  Google Scholar 

  • Yamazoe S, Hasegawa K, Shigemori HZ (2006a) Structure-activity relationship of acetylenes from galls of Hedera rhombea as plant growth inhibitors. Naturforsch C 61:536–540

    CAS  Google Scholar 

  • Yamazoe S, Hasegawa K, Suenaga K, Shigemori H (2006b) Growth inhibitory polyacetylenes from galls of Hedera rhombea Bean. Nat Prod Commun 1:87–94

    CAS  Google Scholar 

  • Yamazoe S, Hasegawa K, Ito J, Mikami Y, Shigemori H (2007) Hederyne A, a new antimicrobial polyacetylene from galls of Hedera rhombea Bean. J Asian Nat Prod Res 9:537–540

    Article  CAS  Google Scholar 

  • Yang LL, Lee CY, Yen KY (2000) Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells. Cancer Lett 157:65–75

    Article  CAS  Google Scholar 

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Acknowledgment

The authors are grateful to Ph.D. Fahmi.Ben.Fredj (University of Tsukuba) for the statistical analysis of this research.

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Authors and Affiliations

  1. Alliance for Research on North Africa, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8572, Ibaraki, Japan

    Parida Yamada, Junkyu Han & Hiroko Isoda

  2. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8572, Ibaraki, Japan

    Takako Ono, Hideyuki Shigemori, Junkyu Han & Hiroko Isoda

Authors
  1. Parida Yamada
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  2. Takako Ono
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  3. Hideyuki Shigemori
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  4. Junkyu Han
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  5. Hiroko Isoda
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Correspondence to Hiroko Isoda.

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Yamada, P., Ono, T., Shigemori, H. et al. Inhibitory effect of tannins from galls of Carpinus tschonoskii on the degranulation of RBL-2H3 Cells. Cytotechnology 64, 349–356 (2012). https://doi.org/10.1007/s10616-012-9457-y

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