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In vitro studies on chemoprotective effect of borax against aflatoxin B1-induced genetic damage in human lymphocytes

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Abstract

A common dietary contaminant, aflatoxin B1 (AFB1), has been shown to be a potent mutagen and carcinogen in humans and many animal species. Since the eradication of AFB1 contamination in agricultural products has been rare, the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Boron compounds like borax (BX) and boric acid are the major components of industry and their antioxidant role has recently been reported. In the present report, we evaluated the capability of BX to inhibit the rate of micronucleus (MN) and sister chromatid exchange (SCE) formations induced by AFB1. There were significant increases (P < 0.05) in both SCE and MN frequencies of cultures treated with AFB1 (3.12 ppm) as compared to controls. However, co-application of BX (1, 2 and 5 ppm) and AFB1 resulted in decreases of SCE and MN rates as compared to the group treated with AFB1 alone. Borax gave 30–50 % protection against AFB1 induced SCEs and MNs. In conclusion, the support of borax was especially useful in aflatoxin-toxicated blood tissue. Thus, the risk on target tissues of AFB1 could be reduced and ensured early recovery from its toxicity.

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Acknowledgments

We are grateful to volunteers for the blood samples. The author is grateful to Dr. Savaş Yeşilyurt for his proofreading the article.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Sciences, Erzurum Technical University, Erzurum, Turkey

    Hasan Turkez

  2. Department of Biology, Faculty of Science, Atatürk University, 25240, Erzurum, Turkey

    Fatime Geyikoğlu

  3. Department of Microbiology, Medical Faculty, Kahramanmaraş Sütçü İmam University, 46100, Kahramanmaraş, Turkey

    Ebubekir Dirican

  4. Department of Medical Genetics, Medical Faculty, Atatürk University, 25240, Erzurum, Turkey

    Abdulgani Tatar

Authors
  1. Hasan Turkez
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  2. Fatime Geyikoğlu
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  3. Ebubekir Dirican
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  4. Abdulgani Tatar
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Correspondence to Ebubekir Dirican.

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Turkez, H., Geyikoğlu, F., Dirican, E. et al. In vitro studies on chemoprotective effect of borax against aflatoxin B1-induced genetic damage in human lymphocytes. Cytotechnology 64, 607–612 (2012). https://doi.org/10.1007/s10616-012-9454-1

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  • DOI: https://doi.org/10.1007/s10616-012-9454-1

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