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Cytotechnology

, Volume 64, Issue 6, pp 711–718 | Cite as

Characterization of Bombyx mori nucleopolyhedrovirus with a knockout of Bm17

  • Hongxing Shen
  • Yang Zhou
  • Wen Zhang
  • Bin Nin
  • Hua Wang
  • Xiaochun Wang
  • Shihe Shao
  • Huiqing Chen
  • Zhongjian Guo
  • Xiaoyong Liu
  • Qin Yao
  • Keping Chen
Original Research

Abstract

Open reading frame 17 (Bm17) gene of Bombyx mori nucleopolyhedrovirus is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this report, transient-expression and superinfection assays indicated that BM17 localized in the nucleus and cytoplasm of infected BmN cells. To determine the role of Bm17 in baculovirus life cycle, we constructed a Bm17 knockout virus and characterized its properties in cells. Analysis of the production and infection of budded virions, the level of viral DNA replication revealed showed that there was no significant difference among the mutant, the control, and the Bm17 repaired virus strains. These results suggest that BM17 is not essential for virus replication in cultured cells.

Keywords

Baculovirus Bm17 BmNPV DNA virus Gene knock-out 

Notes

Acknowledgments

This work was supported by National Basic Research Program of China (No. 2012CB114604), Natural Science Foundation of China (31101673), Natural Science Foundation of China (30871826) and the Senior Talent Foundation of Jiangsu University (1281270014).

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Hongxing Shen
    • 1
  • Yang Zhou
    • 2
  • Wen Zhang
    • 1
  • Bin Nin
    • 1
  • Hua Wang
    • 1
  • Xiaochun Wang
    • 1
  • Shihe Shao
    • 1
  • Huiqing Chen
    • 2
  • Zhongjian Guo
    • 2
  • Xiaoyong Liu
    • 2
  • Qin Yao
    • 2
  • Keping Chen
    • 2
  1. 1.School of Medical Science and Laborarory MedicineJiangsu UniversityZhenjiangPeople’s Republic of China
  2. 2.Institute of Life SciencesJiangsu UniversityZhenjiangPeople’s Republic of China

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