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Human amniotic fluid-derived c-kit+ and c-kit− stem cells: growth characteristics and some differentiation potential capacities comparison

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Abstract

Amniotic fluid (AF) contains heterogeneous and multipotential cell types. A pure mesenchymal stem cells group can be sorted from AF using flow cytometry. In order to evaluate a possible therapeutic application of these cells, the human AF-derived c-kit+ stem cells (c-kit+ AFS) were compared with the c-kit− (unselected) stem cells (c-kit− AFS). Our findings revealed that the optimal period to obtain c-kit+ AFS cells was between 16 and 22 weeks of gestation. Following cell sorting, c-kit+ AFS cells shared similar morphological and proliferative characteristics as the c-kit− AFS cells. Both c-kit+ and c-kit− AFS cells had the characteristics of mesenchymal stem cells through surface marker identification by flow cytometric and immunocytochemical analysis. Both c-kit+ and c-kit− AFS cells could differentiate along adipogenic and osteogenic lineages. However, the myocardial differentiation capacity was enhanced in c-kit+ AFS cells by detecting GATA-4, cTnT, α-actin, Cx43 mRNA and protein expression after myocardial induction; whereas induced c-kit− AFS cells were only detected with GATA-4 mRNA and protein expression. The c-kit+ AFS cells could have potential clinical application for myogenesis in cardiac regenerative therapy.

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Acknowledgments

The authors wish to thank Yajun Guo, Sheng hou, Xin Tong, Lei Zheng and Liangliang Wu for expert technical assistance.

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Authors and Affiliations

  1. Department of Cardiology, First Affiliated Hospital of Chinese PLA General Hospital, 51 Fucheng Road, Haidian District, Beijing, 100048, China

    Jing Bai

  2. Institute of Geriatric Cardiology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, China

    Yiru Wang, Lifeng Liu, Jie Chen & Yu Wang

  3. Direction Center of Family Planing, Haidian Maternal and Children’s Hospital, 33 Haidian Southroad, Haidian District, Beijing, 100080, China

    Wenlan Yang

  4. Department of Cardiology, Navy General Hospital, 6 Fucheng Road, Haidian District, Beijing, 100048, China

    Lianru Gao

Authors
  1. Jing Bai
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  2. Yiru Wang
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  3. Lifeng Liu
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  4. Jie Chen
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  5. Wenlan Yang
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  6. Lianru Gao
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  7. Yu Wang
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Corresponding author

Correspondence to Yu Wang.

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Jing Bai and Yiru Wang have the same contribution to this manuscript.

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Bai, J., Wang, Y., Liu, L. et al. Human amniotic fluid-derived c-kit+ and c-kit− stem cells: growth characteristics and some differentiation potential capacities comparison. Cytotechnology 64, 577–589 (2012). https://doi.org/10.1007/s10616-012-9441-6

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  • DOI: https://doi.org/10.1007/s10616-012-9441-6

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