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Cytotechnology

, Volume 64, Issue 4, pp 477–484 | Cite as

Isolation and characterization of cancer stem cells from cervical cancer HeLa cells

  • Song-Ling Zhang
  • Yi-Shu Wang
  • Tong Zhou
  • Xiao-Wei Yu
  • Zhen-Tong Wei
  • Yu-Lin Li
Original Research

Abstract

Cervical cancer is one of the most common gynecologic malignancies and poses a serious health problem worldwide. Identification and characterization of cervical cancer stem cells may facilitate the development of novel strategies for the treatment of advanced and metastatic cervical cancer. Breast cancer-resistance protein (Bcrp1)-positive cells were selected from a population of parent HeLa cells using flow cytometry. The invasion capacity of Bcrp1-positive and -negative cells was analyzed with a Boyden chamber invasion test. The tumorigenicity of these cells was determined by in vivo transplantation in non-obesity diabetes/severe combined immunodeficiency (NOD/SCID) mice. The Bcrp1-positive subpopulation accounted for about 7% of the parent HeLa cell population. The proliferative capacity of the Bcrp1-positive cells was greater than that of the Bcrp1-negative cells (P < 0.05). In the invasion assay, the Bcrp1-positive cells demonstrated a greater invasive capacity through the artificial basement membrane than their Bcrp1-negative counterparts. Following transplantation of 104 cells, only the Bcrp1-positive cells formed tumors in NOD/SCID mice. When 105 or 106 cells were transplanted, the tumor incidence and the tumor mass were greater in the Bcrp1-positive groups than those in the Bcrp1-negative groups (P < 0.05). The Bcrp1-positive subpopulation cervical cancer stem cells.

Keywords

Cervical cancer Cancer stem cells Bcrp1 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (30901589).

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Department of Gynecology and ObstetricsFirst Hospital of Jilin UniversityChangchunPeople’s Republic of China
  2. 2.Key Laboratory of PathobiologyMinistry of Education, Jilin UniversityChangchunPeople’s Republic of China
  3. 3.Norman Bethune College of Jilin UniversityChangchunPeople’s Republic of China

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