Abstract
The identification of egg extracts with the ability to maintain and enhance the survival and differentiation of cells would be widely useful in cellular biology research. In this study, we compared the different abilities of spleen cells to survive and differentiate in vivo after permeabilization by five different types of egg extracts. Five types of egg extracts were prepared. The spleen cells from male GFP-transgenic mice were permeabilized by the extracts for 30 min, cultured for 12 days, and then transfused into irradiated female mice. At varying days after transplantation, the percentage of GFP-expressing surviving spleen cells was detected in the peripheral blood by flow cytometry. At 120 days after transplantation, bone marrow cells from the female mice were analyzed for the presence of cells containing the Y chromosome. Surviving GFP-positive spleen cells that had been permeabilized with either chicken-egg-white or whole-egg extracts could be detected in the female mice after transplantation. A lower percentage of GFP-positive cells was also detected after permeabilization by the other extracts tested, and no GFP-positive cells were found in the female mouse transfused with spleen cells permeabilized with Hank’s Buffered Salt Solution (HBSS) as a control. At 120 days after transplantation, the percentage of cells containing a Y chromosome in the bone marrow positively correlated with the percentage of GFP-positive cells in the peripheral blood. After permeabilization by chicken-egg-white or whole-egg extracts, spleen cells demonstrated significantly enhanced survival and differentiation functions compared with the spleen cells treated with the other egg extracts tested. These results show that chicken-egg-white and whole-egg extracts have roles in maintaining and enhancing the survival and differentiation of spleen cells. Therefore, these two types of extracts may be of future use in maintaining the function of stem cells.
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References
Alberio R, Johnson AD, Stick R, Campbell KH (2005) Differential nuclear remodeling of mammalian somatic cells by Xenopus laevis oocyte and egg cytoplasm. Exp Cell Res 307:131–141
Balic A, Mina M (2010) Identification of secretory odontoblasts using DMP1-GFP transgenic mice. Bone 48:927–937
Cameron LA, Poccia DL (1994) In vitro development of the sea urchin male pronucleus. Dev Biol 162:568–578
Cho HJ, Lee CS, Kwon YW, Paek JS, Lee SH, Hur J, Lee EJ, Roh TY, Chu IS, Leem SH, Kim Y, Kang HJ, Park YB, Kim HS (2010) Induction of pluripotent stem cells from adult somatic cells by protein-based reprogramming without genetic manipulation. Blood 116:386–395
Danilchick M, Peng HB, Kay BK (1991) Xenopus laevis: Practical uses in cell and molecular biology. Pictorial collage of embryonic stages. Methods Cell Biol 36:679–681
Freberg CT, Dahl JA, Timoskainen S, Collas P (2007) Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract. Mol Biol Cell 18:1543–1553
Hansis C, Barreto G, Maltry N, Niehrs C (2004) Nuclear reprogramming of human somatic cells by xenopus egg extract requires BRG1. Curr Biol 14:1475–1480
Huang X, Cho S, Spangrude GJ (2007) Hematopoietic stem cells: generation and self-renewal. Cell Death Differ 14:1851–1859
Iwao Y, Katagiri C (1984) In vitro induction of sperm nucleus decondensation by cytosol from mature toad eggs. J Exp Zool 230:115–124
Leno GH (1998) Cell-free systems to study chromatin remodeling. Methods Cell Biol 53:497–515
Lohka MJ, Masui Y (1983) Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components. Science 220:719–721
Miyamoto K, Tsukiyama T, Yang Y, Li N, Minami N, Yamada M, Imai H (2009) Cell-free extracts from mammalian oocytes partially induce nuclear reprogramming in somatic cells. Biol Reprod 80:935–943
Ning L, Goossens E, Geens M, Van Saen D, Van Riet I, He D, Tournaye H (2010) Mouse spermatogonial stem cells obtain morphologic and functional characteristics of hematopoietic cells in vivo. Hum Reprod 25:3101–3109
Smeti I, Savary E, Capelle V, Hugnot JP, Uziel A, Zine A (2010) Expression of candidate markers for stem/progenitor cells in the inner ears of developing and adult GFAP and nestin promoter-GFP transgenic mice. Gene Expr Patterns 11:22–32
Takaishi S, Shibata W, Tomita H, Jin G, Yang X, Ericksen R, Dubeykovskaya Z, Asfaha S, Quante M, Betz KS, Shulkes A, Wang TC (2010) In vivo analysis of mouse gastrin gene regulation in enhanced GFP-BAC transgenic mice. Am J Physiol Gastrointest Liver Physiol 300:G334–G344
Taranger CK, Noer A, Sorensen AL, Hakelien AM, Boquest AC, Collas P (2005) Induction of dedifferentiation, genomewide transcriptional programming, and epigenetic reprogramming by extracts of carcinoma and embryonic stem cells. Mol Biol Cell 16:5719–5735
Ulitzur N, Gruenbaum Y (1989) Nuclear envelope assembly around sperm chromatin in cell-free preparations from Drosophila embryos. FEBS Lett 259:113–116
Wilkosz S, Pullen N, de-Giorgio-Miller A, Ireland G, Herrick S (2011) Cellular exchange in an endometriosis-adhesion model using GFP transgenic mice. Gynecol Obstet Invest 72:90–97
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This work was supported by the National Natural Science Foundation of China (31172170), 973 Projects (2012CB518106) and special funding by the China Postdoctoral Science Foundation (201104748).
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Ruan, GP., Wang, JX., Pang, RQ. et al. Treatment with chicken-egg-white or whole-egg extracts maintains and enhances the survival and differentiation of spleen cells. Cytotechnology 64, 541–551 (2012). https://doi.org/10.1007/s10616-012-9431-8
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DOI: https://doi.org/10.1007/s10616-012-9431-8