, Volume 64, Issue 2, pp 139–144 | Cite as

Optimization of condition(s) towards establishment of primary islet cell cultures from WNIN/Ob mutant rat

  • V. Venkatesan
  • M. Chalsani
  • S. S. Nawaz
  • R. R. Bhonde
  • S. S. Challa
  • G. Nappanveettil
Brief Report


WNIN/Ob, a mutant rat strain, developed at the National Center for Laboratory Animal Sciences (NCLAS) facility of National Institute of Nutrition (NIN), is a new animal model to study the metabolic syndrome. These animals have 47% fat in their body and isolation of islets from these animals were compounded due to the formation of amorphous viscous and jelly like material which reduced the islet yield. However, islets isolated from WNIN adult (≥12 months) control rats gave a good islet recovery, under standard isolation procedures using collagenase digestion. In the present study we optimized culture conditions in WNIN/Ob rats to isolate islets with higher yield, and also established primary islet cell cultures from these mutant rats, retaining cellular integrity and functionality.


Obese rats Collagenase Islet isolation Islet cell culture Viability and islet function 



Inbred Wistar rat strain established at NIN


Wistar rats raised in the National Institute of Nutrition, with obesity trait


National Center for Laboratory Animal Sciences


National Institute of Nutrition




Krebs–Ringer bicarbonate HEPES



We thank Director of National Institution of Nutrition for providing us the infrastructure facility as well as intramural grant support to carry out this work. The manuscript corrections and support extended by Ms K. Sowmya is acknowledged.


  1. Amoli MM, Moosavizadeh R, Larijani B (2005) Optimizing conditions for rat pancreatic islets isolation. Cytotechnology 48:75–78. doi: 10.1007/s10616-005-3586-5 CrossRefGoogle Scholar
  2. Bandaru P, Rajkumar H, Nappanveettil G (2011) Altered or impaired immune response upon vaccination in WNIN/Ob rats. Vaccine 16:3038–3042. doi: 10.1016/j.vaccine.2011.01.107 CrossRefGoogle Scholar
  3. Banerjee M, Bhonde RR (2003) Islet generation from intra islet precursor cells of diabetic pancreas: in vitro studies depicting in vivo differentiation. JOP 4:137–145Google Scholar
  4. Giridharan NV (1998) Animal models of obesity and their usefulness in molecular approach to obesity. Indian J Med Res 108:225–242Google Scholar
  5. Giridharan NV, Harishankar N, Satyavani M (1996) A new rat model for the study of obesity. Scand J Anim Sci 23:131–137Google Scholar
  6. Gotoh M, Maki T, Kiyozumi T, Satomi S, Monaco AP (1985) An improved method for isolation of mouse pancreatic islets. Transplantation 40:437–438CrossRefGoogle Scholar
  7. Gray DWR, Mc Shane P, Grant A, Morris PJ (1984) A method for isolation of islets of langerhans from the human pancreas. Diabetes 33:1055–1061. doi: 10.2337/diabetes.33.11.105 CrossRefGoogle Scholar
  8. Hara Y, Taniguchi H, Ishihara K et al (1989) Simple and easy method for harvesting of a large number of isolated islets and their function. Transplant Proc 21:2632–2634Google Scholar
  9. Kiran SG, Dorisetty RK, Umrani MR, Boindala S, Bhonde RR, Chalsani M, Singh H, Venkatesan V (2011) Pyridoxal 5′ phosphate protects islets against streptozotocin-induced beta-cell dysfunction–in vitro and in vivo. Exp Biol Med 236:456–465. doi: 10.1258/ebm.2011.010361 CrossRefGoogle Scholar
  10. Kneteman N, Alderson D, Scharp DW (1987) The isolation and purification of human pancreatic islets. Transplant Proc 19:3469–3470Google Scholar
  11. Lui M, Shapiro ME (1995) A new method for isolation of murine islets with markedly improved yields. Transplant Proc 27:3208–3210Google Scholar
  12. Madhira SL, NappanVeettil G, Kodavalla V, Venkatesan V (2011) Comparison of adipocyte–specific gene expression from WNIN/Ob mutant obese rats, lean control and parental control. Mol Cell Biochem 357:217–225. doi: 10.1007/s11010-011-0892-4 CrossRefGoogle Scholar
  13. Perdrizet GA, Rewinski MJ, Bartus SA, Hull D, Schweizer RT, Scharp DW (1995) Albumin improves islet isolation: specific versus nonspecific effects. Transplant Proc 27:3400–3402Google Scholar
  14. Reddy GB, Vasireddy V, Mandal MN, Tiruvalluru M, Wang XF, Jablonski MM, Nappanveettil G, Ayyagari R (2009) A novel rat model with obesity-associated retinal degeneration. Invest Ophthalmol Vis Sci 50:3456–3463. doi: 10.1167/iovs.08-2498 Google Scholar
  15. Sakamuri VP, Ananthathmakula P, Veettil GN, Ayyalasomayajula V (2011) Vitamin A decreases pre-receptor amplification of glucocorticoids in obesity—study on the effect of Vitamin A on 11 beta—hydroxysteroid dehydrogenase type 1 activity in liver and visceral fat of WNIN/Ob obese rats. Nutr J 10:70. doi: 10.1186/1475-2891-10-70
  16. Shewade Y, Umrani M, Bhonde RR (1999) Large-scale isolation of islets by tissue culture of adult mouse pancreas. Transplant Proc 31:1721–1723CrossRefGoogle Scholar
  17. Vijayalakshmi V, Naseem B, Khan AA, Capoor AK, Habibullah CM (2004) Comparison of biochemical and cytotoxic functions of hepatocytes from goat, pig and human fetuses. J Gastroenterol Hepatol 19:1029–1035. doi: 10.1111/j.1440-1746.2004.03402.x Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • V. Venkatesan
    • 1
  • M. Chalsani
    • 1
  • S. S. Nawaz
    • 1
  • R. R. Bhonde
    • 2
  • S. S. Challa
    • 1
  • G. Nappanveettil
    • 1
  1. 1.National Centre for Laboratory Animal Science, National Institute of NutritionJamai OsmaniaHyderabadIndia
  2. 2.Manipal Institute of Regenerative MedicineBangaloreIndia

Personalised recommendations