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The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions

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Abstract

The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extracts (Sp) were investigated in human lymphocytes. The cells were treated with 1.5, 3.0 and 6.0 μL/mL concentrations of Sp leaf extracts for 24 and 48 h treatment periods in the absence and presence of metabolic activator (S9mix). In the absence of S9mix, Sp alone did not induce chromosome aberrations and formation of micronucleus while inducing the mean sister chromatid exchange at the highest concentration. In addition, Sp decreased the mutagenic effect of mitomycin-c. Sp alone showed a cytotoxic effect determined by a decrease in the proliferation index, mitotic index and nuclear division index. On the other hand a mixture of Sp and mitomycin-c resulted in a higher cytotoxic effect especially for 48 h treatment period. In the presence of S9mix, Sp was not genotoxic and cytotoxic however, it showed an anti-genotoxic effect by decreasing the effects of cyclophosphamide.

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References

  • Ahmad VU, Arshad S, Bader S, Iqbal S, Khan A, Khan SS, Hussain J, Tareen RB, Ahmed A (2008) New terpenoids from Stachys parviflora Benth. Fitoterapia 79:595–597

    Article  Google Scholar 

  • Aksoy C, Yucecan S, Ciftci N, Tayfur M, Akgun B, Tasci N (1988) Kanser hastalığında tedavi amacıyla kullanılan yöresel bitkiler (The local plants that were used in cancer therapy). Beslenme ve Diyet Dergisi (J Nutr Diet) 17:111–120

    Google Scholar 

  • Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, Merlo F, Natarajan AT, Norppa H, Shuker DEG, Tice R, Waters MD, Aitio A (2000) IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutat Res 463:111–172

    Article  CAS  Google Scholar 

  • Ali NA, Marongiu B, Piras A, Porcedda S, Falconieri D, Molicotti P, Zanetti S (2010) Essential oil composition of leaves of Stachys yemenensis obtained by supercritical CO2. Nat Prod Res 24:1823–1829

    Article  Google Scholar 

  • Amirghofran Z, Bahmani M, Azadmehr A, Javidnia K (2006) Anticancer effects of various Iranian native medicinal plants on human tumor cell lines. Neoplasma 53:428–433

    CAS  Google Scholar 

  • Amirghofran Z, Bahmani M, Azadmehr A, Javidnia K (2007) Immunomodulatory and apoptotic effects of Stachys obtusicrena on proliferative lymphocytes. Med Sci Monit 13:145–150

    Google Scholar 

  • Arslan M, Topaktas M, Rencuzogullari E (2008) The effects of boric acid on sister chromatid exchanges and chromosome aberrations in cultured human lymphocytes. Cytotechnology 56:91–96

    Article  CAS  Google Scholar 

  • Azirak S, Rencuzogullari E (2008) The in vivo genotoxic effects of carvacrol and thymol in rat bone marrow cells. Environ Toxicol 23:728–735

    Article  CAS  Google Scholar 

  • Basaran AA, Yu TW, Plewa MJ, Anderson D (1996) An investigation of some Turkish herbal medicines in Salmonella typhimurium and in the COMET assay in human lymphocytes. Teratog Carcinog Mutagen 16:125–138

    Article  CAS  Google Scholar 

  • Bianchini F, Vainio H (2001) Allium vegetables and organosulfur compounds: do they help prevent cancer? Environ Health Perspect 109:893–902

    Article  CAS  Google Scholar 

  • Buyukleyla M, Rencuzogullari E (2009) The effects of thymol on sister chromatid exchange, chromosome aberration and micronucleus in human lymphocytes. Ecotoxicol Environ Safety 72:943–947

    Article  CAS  Google Scholar 

  • Ćavar S, Maksimović M, Vidic D, Šolić ME (2010) Chemical composition of the essential oil of Stachys menthifolia. Vis Pharm Biol 48:170–176

    Article  Google Scholar 

  • Chiang TA, Wu PF, Wang LF, Lee H, Lee CH, Ko YC (1997) Mutagenicity and polycylic aromatic hydrocarbon content of fumes from heated cooking oils produced in Taiwan. Mutat Res 381:157–161

    CAS  Google Scholar 

  • Couladis M, Tzakou O, Verykokidou E, Harvala C (2003) Screening of some Greek aromatic plants for antioxidant activity. Phytother Res 17:194–195

    Article  Google Scholar 

  • Delazar A, Celik S, Gokturk RS, Unal O, Nahar L, Sarker SD (2005) Two acylated flavonoid glycosides from Stachys bombycina, and their free radical scavenging activity. Pharmazie 60:878–880

    CAS  Google Scholar 

  • Delazar A, Delnavazi MR, Nahar L, Moghadam SB, Mojarab M, Gupta A, Williams AS, Mukhlesur-Rahman M, Sarker SD (2011) Lavandulifolioside B: a new phenylethanoid glycoside from the aerial parts of Stachys lavandulifolia Vahl. Nat Prod Res 25:8–16

    Article  CAS  Google Scholar 

  • Evans HJ (1984) Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests. In: Kilbey BJ, Legator M, Nichols W, Ramel C (eds) Handbook of mutagenicity test procedures, 2nd edn. Elsevier Sciences, Amsterdam, pp 405–427

    Google Scholar 

  • Fenech M (2000) The in vitro micronucleus technique. Mutat Res 455:81–95

    CAS  Google Scholar 

  • Giuliani C, Pellegrino RM, Tirillini B, Bini LM (2009) Composition of essential oils from leaves and flowers of Stachys germanica subsp salviifolia (Ten.) gams (Labiatae) and related secretory structures. Nat Prod Commun 4:831–834

    CAS  Google Scholar 

  • Gurley ES, Rahman M, Hossain MJ, Nahar N, Faiz MA, Islam N, Sultana R, Khatun S, Uddin MZ, Haider MS, Islam MS, Ahmed BN, Rahman MW, Mondal UK, Luby SP (2010) Fatal outbreak from consuming Xanthium strumarium seedlings during time of food scarcity in northeastern Bangladesh. PLoS One 5:e9756

    Article  Google Scholar 

  • Haznagy-Radnai E, Czigle S, Zupko I, Falkay G, Mathe I (2008) Comparison of antioxidant activity in enzyme-independent system of six Stachys species. Fitoterapia 77:521–524

    Article  Google Scholar 

  • Hollman PCH, Hertog MGL, Katan MB (1996) Analysis and health effects of flavonoids. Food Chem 57:43–46

    Article  CAS  Google Scholar 

  • Ivanova A, Nechev J, Tsvetkova I, Stefanov K, Popov S (2009) Chemical composition of the halophyte plant Stachys maritima Gouan from the Bulgarian Black Sea coast. Nat Prod Res 23:448–454

    Article  CAS  Google Scholar 

  • Karakaya S, Kavas A (1999) Antimutagenic activities of some foods. J Sci Food Agric 79:237–242

    Article  CAS  Google Scholar 

  • Kayraldiz A, Yavuz-Kocaman A, Rencuzogullari E, Istifli ES, Ila HB, Topaktas M, Daglioglu YK (2010) The genotoxic and antigenotoxic effects of Aloe vera leaf extract in vivo and in vitro. Turk J Biol 34:235–246

    CAS  Google Scholar 

  • Khanum F, Anilkumar KR, Viswanathan KR (2004) Anticarcinogenic properties of garlic: a review. Crit Rev Food Sci Nutr 44:479–488

    Article  CAS  Google Scholar 

  • Kirsch-Volders M, Sofuni T, Aardemac M (2003) Report from the in vitro micronucleus assay working group. Mutat Res 540:153–163

    CAS  Google Scholar 

  • Kocaman AY, Rencuzogullari E, Topaktas M, Istifli ES, Buyukleyla M (2011) The effects of 4-thujanol on chromosome aberrations, sister chromatid exchanges and micronucleus in human peripheral blood lymphocytes. Cytotechnology doi:10.1007/s10616-011-9372-7

  • Kukic J, Petrovic S, Niketiv M (2006) Antioxidant activity of four endemic Stachys taxa. Biol Pharm Bull 29:725–729

    Article  CAS  Google Scholar 

  • Lenherr A, Meier B, Sticher O (1984) Modern HPLC as a tool for chemotaxonomical investigations: iridoid glucosides and acetylated flavonoids in the group of Stachys recta. Planta Med 50:403–409

    Article  CAS  Google Scholar 

  • Mace MLJ, Daskal Y, Wray W (1978) Scanning electron microscopy of chromosome aberrations. Mutat Res 52:199–206

    Google Scholar 

  • Madhavi D, Devil KR, Rao KK, Reddy PP (2007) Modulating effect of phyllanthus fruit extract against lead genotoxicity in germ cells of mice. Environ Biol 28:115–117

    CAS  Google Scholar 

  • Madle S, Beek B, Nowak C (1993) Zum Verstandnis von Chromosomenmutationstests an Somazellen. In: Fahrig R (ed) Mutationsforschung und Genetische Toxicologie. Wissenschaftliche Buchgesellschaft, Germany, pp 224–242

    Google Scholar 

  • Maron DM, Ames BN (1983) Revised method for he Salmonella mutagenicity test. Mutat Res 113:173–215

    CAS  Google Scholar 

  • Mendelsohn ML (1992) Antimutagenic effects in humans. Mutat Res 267:257–264

    CAS  Google Scholar 

  • Milner JA (2001) A historical perspective on garlic and cancer. J Nutr 131:1027–1031

    Google Scholar 

  • Murata T, Endo Y, Miyase T, Yoshizaki F (2008) Iridoid glycoside constituents of Stachys lanata. J Nat Prod 71:1768–1770

    Article  CAS  Google Scholar 

  • Ozturk M, Duru ME, Aydogmus-Ozturk F, Harmandar M, Mahlicli M, Kolak U, Ulubelen A (2009) GC-MS analysis and antimicrobial activity of essential oil of Stachys cretica subsp Smyrnaea. Nat Prod Commun 4:109–114

    CAS  Google Scholar 

  • Paz-y-Miño C, Bustamante G, Sánchez ME, Leone PE (2002) Cytogenetic monitoring in a population occupationally exposed to pesticides in Ecuador. Environ Health Perspect 110:1077–1080

    Article  Google Scholar 

  • Perry PE, Thompson EJ (1984) The methodology of sister chromatid exchanges. In: Kilbey BJ, Legator M, Nichols W, Ramel C (eds) Handbook of mutagenicity test procedures, 2nd edn. Elsevier Sciences, Amsterdam, pp 495–529

    Google Scholar 

  • Qu YH, Xu GX, Zhou JZ, Chen TD, Zhu LF, Shields PG, Wang HW, Gao YT (1992) Genotoxicity of heated cooking oil vapors. Mutat Res 298:105–111

    Article  CAS  Google Scholar 

  • Rencuzoğullari E, Azirak S, Canimoglu S, Parlak S, Buyukleyla M (2009) Effects of natamycin on sister chromatid exchanges, chromosome aberrations and micronucleus in human lymphocytes. Drug Chem Toxicol 32:47–52

    Article  Google Scholar 

  • Roncada T, Vicentini VE, Mantovani MS (2004) Possible modulating actions of plant extracts on the chromosome breaking activity of MMC and Ara-C in human lymphocytes in vitro. Toxicol Invit 18:617–622

    CAS  Google Scholar 

  • Sajjadi MH, Amiri H (2007) Chemical constituents of the essential oils of different stages of the growth of Stachys lavandulifolia Vahl. from Iran. Pak J Biol Sci 10:2784–2786

    Article  Google Scholar 

  • Savic MM, Kukic JM, Grayer RJ, Milinkovic MM, Marin PD, Divljakovic J, Van-Linn M, Cook JM, Petrovic SD (2010) Behavioural characterization of four endemic Stachys taxa. Phytother Res 24:1309–1316

    Article  CAS  Google Scholar 

  • Serbetci T, Demirci B, Guzel CB, Kultur S, Erguven M, Baser KH (2010) Essential oil composition, antimicrobial and cytotoxic activities of two endemic Stachys cretica subspecies (Lamiaceae) from Turkey. Nat Prod Commun 5:1369–1374

    CAS  Google Scholar 

  • Shin TY (2004) Stachys riederi inhibits mast cell-mediated acute and chronic allergic reactions. Immunopharmacol Immunotoxicol 26:621–630

    Article  Google Scholar 

  • Sowjanya BL, Devi KR, Madhavi D (2009) Modulatory effects of garlic extract against the cyclophosphamide induced genotoxicity in human lymphocytes in vitro. J Environ Biol 30:663–666

    CAS  Google Scholar 

  • Speit G, Haupter S (1985) On the mechanisms of differential Giemsa staining of bromodeoxyuridine-substituted chromosomes II. Differences between the demonstration of sister chromatid differentiation and replication patterns. Hum Genet 70:126–129

    Article  CAS  Google Scholar 

  • Tepe B, Degerli S, Arslan S, Malatyali E, Sarikurkcu C (2011) Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica. Fitoterapia 82:237–246

    Article  CAS  Google Scholar 

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Acknowledgments

This study was supported by C. U. Research Fund. FEF2009YL1.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science and Letters, Adiyaman University, 02030, Adiyaman, Turkey

    Eyyup Rencuzogullari

  2. Department of Biology, Natural and Applied Science Institute, Çukurova University, 01330, Adana, Turkey

    Ayse Mine Yildiz & Mehmet Buyukleyla

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  1. Eyyup Rencuzogullari
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  3. Mehmet Buyukleyla
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Correspondence to Eyyup Rencuzogullari.

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Rencuzogullari, E., Yildiz, A.M. & Buyukleyla, M. The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions. Cytotechnology 64, 83–94 (2012). https://doi.org/10.1007/s10616-011-9396-z

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