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Cytotechnology

, Volume 64, Issue 3, pp 241–247 | Cite as

Effect of physiological levels of phytoestrogens on mouse oocyte maturation in vitro

  • Naoko Yoshida
  • Katsushige Mizuno
JAACT Special Issue

Abstract

Phytoestrogens are a group of naturally occurring compounds that have weak estrogenic activity. Genistein and daidzein are major phytoestrogens produced by soybeans. It has been reported previously that at high concentration, some phytoestrogens inhibit cell cycle progression of mouse germinal vesicle (GV) oocytes, but the environmentally relevant level is much lower. Here we show the effects of low concentrations of the isoflavones genistein, daidzein and the daidzein metabolite, equol, on mouse oocyte maturation. GV oocytes denuded of cumulus cells were cultured in TaM medium containing low levels (5 μM) of genistein, daidzein. or equol. In all cases, the oocytes underwent normal GV break down, first polar body extrusion and became arrested at metaphase II (mII). As judged by fluorescence microscopy, the treated mII oocytes exhibited normal distributions of actin microfilaments, cortical granules and metaphase spindle formation with condensed metaphase chromatin. Moreover, mRNA expression levels of the cytostatic factors Emi2 and Mos were similar to those of their respective controls. These data suggest that exposure of maturing GV oocytes to environmental levels of genistein, daidzein or equol in vitro do not cause negative effects on maturation to produce mII oocytes.

Keywords

Genistein Daidzein Equol Mouse Oocyte maturation Meiosis Metaphase arrest 

Abbreviations

GV

Germinal vesicle

GVBD

Germinal vesicle breakdown

IVM

In vitro maturation

mI

First metaphase

mII

Second metaphase

CSF

Cytostatic factor

Notes

Acknowledgments

We appreciate Dr. Tony Perry for reading the manuscript and giving us critical comments.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of Biomedical Sciences, College of Life SciencesRitsumeikan UniversityShigaJapan

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