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Transition of basic protein during spermatogenesis of Fenneropenaeus chinensis (Osbeck, 1765)

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Abstract

According to the ultrastructural characteristic observation of the developing male germ cells, spermatogenesis of the crustacean shrimp, Fenneropenaeus chinensis, is classified into spermatogonia, primary spermatocytes, secondary spermatocyte, four stages of spermatids, and mature sperm. The basic protein transition during its spermatogenesis is studied by transmission electron microscopy of ammoniacal silver reaction and immunoelectron microscopical distribution of acetylated histone H4. The results show that basic protein synthesized in cytoplasm of spermatogonia is transferred into the nucleus with deposition on new duplicated DNA. In the spermatocyte stage, some nuclear basic protein combined with RNP is transferred into the cytoplasm and is involved in forming the cytoplasmic vesicle clumps. In the early spermatid, most of the basic protein synthesized in the new spermatid cytoplasm is transferred into the nucleus, and the chromatin condensed gradually, and the rest is shifted into the pre-acrosomal vacuole. In the middle spermatid, the nuclear basic protein linked with DNA is acetylated and transferred into the proacrosomal vacuole and assembled into the acrosomal blastema. At the late spermatid, almost all of the basic protein in the nucleus has been removed into the acrosome. During the stage from late spermatid to mature sperm, some de novo basic proteins synthesized in the cytoplasm belt transfer into the nucleus without a membrane and almost all deposit in the periphery to form a supercoating. The remnant histone H4 accompanied by chromatin fibers is acetylated in the center of the nucleus, leading to relaxed DNA and activated genes making the nucleus non-condensed.

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Acknowledgments

We thank the National Natural Science Foundation (China, 30371115), the Natural Science Foundation from the Department of Education of Hebei Province (Hebei China, 2009105), the Research Plan Foundation from Hebei Population and Family Planning Committee (Hebei China, 2009-B16) and the Medical Science Foundation from Public Health Department of Hebei Province (Hebei China, 20090173) for financial support.

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Authors and Affiliations

  1. Hebei University Health Science Center, 071000, Baoding, People’s Republic of China

    Shaoqin Ge, Fei Duan, Yan Wang, Wenyan Li & Yuhua Zhang

  2. College of Life Science, Hebei University, 071000, Baoding, People’s Republic of China

    Shaoqin Ge, Xianjiang Kang, Mingshen Guo & Shumei Mu

  3. Langfang Health Vocational College, 065000, Langfang, People’s Republic of China

    Suixin Wang

  4. Hebei University Health Science Center, Yuhua East Road 342, 071002, Baoding, Hebei Province, China

    Shaoqin Ge

  5. College of Life Science, Hebei University, Wusi East Road 180, 071002, Baoding, Hebei Province, China

    Xianjiang Kang

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  1. Shaoqin Ge
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Correspondence to Shaoqin Ge, Xianjiang Kang or Fei Duan.

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Ge, S., Wang, S., Kang, X. et al. Transition of basic protein during spermatogenesis of Fenneropenaeus chinensis (Osbeck, 1765). Cytotechnology 63, 581–598 (2011). https://doi.org/10.1007/s10616-011-9364-7

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