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Down regulation effect of Rosmarinus officinalis polyphenols on cellular stress proteins in rat pheochromocytoma PC12 cells

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Abstract

Polyphenols are known to exhibit wide spectrum of benefit for brain health and to protect from several neurodegenerative diseases. The present study was sought to determine the neuroprotective effects of Rosmarinus officinalis’ polyphenols (luteolin, carnosic acid, and rosmarinic acid) through the investigation of stress-related proteins. We carried out measurement of the expression of heat-shock protein (Hsp) 47 promoter in heat stressed Chinese hamster ovary transfected cells. We performed proteomic analysis and confirmed gene expression by real time PCR in PC12 cells. Results showed that these compounds modulated significant and different effects on the expression of 4 stress-related proteins: heat shock protein 90 α (Hsp90), Transitional endoplasmic reticulum ATPase (VCP/p97), Nucleoside diphosphate kinase (NDK), and Hypoxia up-regulated protein 1 (HYOU1)) at translational and post translational levels in PC12 cells and they downregulated the expression of Hsp47 activity in Chinese hamster transformed cells. These findings suggest that luteolin, carnosic acid, and rosmarinic acid may modulate the neuroprotective defense system against cellular stress insults and increase neuro-thermotolerance.

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Acknowledgments

This work was partially supported by by Mitsui & Co., Ltd. Environment Fund.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, Alliance for Research on North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki, 305-8572, Japan

    Abdelfatteh E. L. Omri, Junkyu Han & Hiroko Isoda

  2. Unité de Recherche Physico-chimie et Moléculaire, Institut Préparatoire aux Etudes Scientifiques et Techniques IPEST, La Marsa, University of Carthage, 2070, Tunis, Tunisia

    Manef Ben Abdrabbah

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  1. Abdelfatteh E. L. Omri
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  2. Junkyu Han
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  3. Manef Ben Abdrabbah
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  4. Hiroko Isoda
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Correspondence to Hiroko Isoda.

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E. L. Omri, A., Han, J., Ben Abdrabbah, M. et al. Down regulation effect of Rosmarinus officinalis polyphenols on cellular stress proteins in rat pheochromocytoma PC12 cells. Cytotechnology 64, 231–240 (2012). https://doi.org/10.1007/s10616-011-9352-y

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  • DOI: https://doi.org/10.1007/s10616-011-9352-y

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