Abstract
To establish an adequate model to study the proliferation and differentiation of adult caprine skeletal muscle in response to bioactive compounds, a pool of satellite cells (SC) was derived from the rectus abdominis muscle of adult goat. Skeletal muscle contains a population of adult stem cells, named as satellite cells that reside beneath the basal lamina of skeletal muscle fiber and other populations of cells. These SC are multipotent stem cells, since cells cultured in the presence of specific cell lineage inducing cocktails can differentiate into several types of mesenchymal lineage, such as osteocytes and adipocytes. In the present study, we have developed a modified protocol for isolating satellite cells (>90%) and examined their myogenic and contractile properties in vitro.
References
Allen RE, Boxhorn LK (1989) Regulation of skeletal muscle satellite cell proliferation and differentiation by transforming growth factor-beta, insulin-like growth factor I, and fibroblast growth factor. J Cell Physiol 138:311–315
Anderson JE (2000) A role for nitric oxide in muscle repair: Oxide-mediated activation of muscle satellite cells. Mol Biol Cell 11:1859–1874
Asakura A, Komaki M, Rudnicki M (2001) Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation 68:245–253
Byrne KM, Vierck J, Dodson MV (2000) In vitro model of equine muscle regeneration. Equine Vet J 32:401–405
Charge SB, Rudnicki MA (2004) Cellular and molecular regulation of muscle regeneration. Physiol Rev 84:209–238
Chen X, Mao Z, Liu S, Liu H, Wang X, Wu H, Wu Y, Zhao T, Fan W, Li Y, Yew DT, Kindler PM, Li L, He Q, Qian L, Wang X, Fan M (2005) Dedifferentiation of adult human myoblasts induced by ciliary neurotrophic factor in vitro. Mol Biol Cell 16:3140–3151
Clair JA, Meyer-Demarest SD, Ham RG (1992) Improved medium with EGF and BSA for differentiated human skeletal muscle cells. Muscle Nerve 15(7):774–779
Das M, Gregory CA, Molnara P, Riedela LM, Wilsona K, Hickman JJ (2006) A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures. Biomaterials 27:4374–4380
Dodson MV, Mathison BA (1988) Comparison of ovine and rat muscle-derived satellite cells: response to insulin. Tissue Cell 20:909–918
Dodson MV, Martin EL, Brannon MA, Mathison BA, McFarland DC (1987) Optimization of bovine satellite cell-derived myotube formation in vitro. Tissue Cell 19:159–166
Dodson MV, McFarland DC, Grant AL, Doumit ME, Velleman SG (1996) Extrinsic regulation of domestic animal derived satellite cells. Domest Anim Endocrinol 13:107–126
Hembree JR, Hathaway MR, Dayton WR (1991) Isolation and culture of fetal porcine myogenic cells and the effect of insulin, IGF-I, and sera on protein turnover in porcine myotube cultures. J Anim Sci 69:3241–3250
Kawada S, Tachi C, Ishii N (2001) Content and localization of myostatin in mouse skeletal muscles during aging, mechanical unloading and reloading. J Muscle Res Cell Motil 22:627–633
Linge C, Green MR, Brooks RF (1989) A method for removal fibroblasts from Human tissue culture systems. Exp Cell Res 185:519–528
Mau M, Oksbjerg N, Rehfeldt C (2008) Establishment and conditions for growth and differentiation of a myoblast cell line derived from the semimembranosus muscle of newborn piglets. In Vitro Cell Dev Biol Anim 44:1–5
Mcfarland DC (1992) Cell culture as a tool for the study of poultry skeletal muscle development. The Journal of Nutrition. Conference: understanding growth and development, pp 818–829
McFarland DC (1999) Influence of growth factors on poultry myogenic satellite cells. Poult Sci 78:747–758
Rando TA, Blau HM (1994) Primary mouse myoblast purification, characterization and transplantation for cell-mediated gene-therapy. J Cell Biol 125:1275–1287
Roe JA, Harper JM, Buttery PJ (1989) Protein metabolism in ovine primary muscle cultures derived from satellite cells-effects of selected peptide hormones and growth factors. J Endocrinol 122:565–571
Springer ML, Rando T, Blau HM (1997) Isolation and growth of mouse primary myoblasts. “Gene delivery to muscle”. In: Boyle AL (ed) Current protocols in human genetics. Unit 13.4. New York, Wiley
Terekhov SM, Krokhina TB, Shishkin SS, Krakhmaleva IN, Zakharov SF, Ershova ES (2001) Human myoblast culture as muscle stem cells in medical and biological studies. Biol Bull 28(6):630–635
Velleman SG, Liu X, Coy CS, McFarland DC (2004) Effects of syndecan-1 and glypican on muscle cell proliferation and differentiation: implications for possible functions during myogenesis. Poult Sci 83:1020–1027
Wada MR, Inagawa-Ogashiwa M, Shimizu S, Yasumoto S, Hashimoto N (2002) Generation of different fates from multipotent muscle stem cells. Development 129:2987–2995
Yablonka-Reuveni Z, Nameroff M (1990) Temporal differences in desmin expression between myoblasts from embryonic and adult chicken skeletal muscle. Differentiation 45:21–28
Yamanouchi K, Hosoyama T, Murakami Y, Nishihara M (2007) Myogenic and adipogenic properties of goat skeletal muscle stem cells. J Reprod Dev 53:51–58
Yamanouchi K, Hosoyama T, Murakami Y, Nakano S, Nishihara M (2009) Satellite cell differentiation in goat skeletal muscle single fiber culture. J Reprod Dev 55:252–255
Acknowledgment
We thank National Agriculture Innovation Project, Indian council of Agricultural Research (NAIP-ICAR), Government of India, for their financial support during this study.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tripathi, A.K., Ramani, U.V., Ahir, V.B. et al. A modified enrichment protocol for adult caprine skeletal muscle stem cell. Cytotechnology 62, 483–488 (2010). https://doi.org/10.1007/s10616-010-9306-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10616-010-9306-9