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A novel composition for the culture of human adipose stem cells which includes complement C3

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Abstract

Adipose tissue is an easily accessible and abundant source of stem cells. Adipose stem cells (ASCs) are currently being researched as treatment options for repair and regeneration of damaged tissues. The standard culture conditions used for expansion of ASCs contain fetal bovine serum (FBS) which is undefined, could transmit known and unknown adventitious agents, and may cause adverse immune reactions. We have described a novel culture condition which excludes the use of FBS and characterised the resulting culture. Human ASCs were cultured in the novel culture medium, which included complement protein C3. These cultures, called C-ASCs, were compared with ASCs cultured in medium supplemented with FBS. Analysis of ASCs for surface marker profile, proliferation characteristics and differentiation potential indicated that the C-ASCs were similar to ASCs cultured in medium containing FBS. Using a specific inhibitor, we show that C3 is required for the survival of C-ASCs. This novel composition lends itself to being developed into a defined condition for the routine culture of ASCs for basic and clinical applications.

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Acknowledgments

This work was supported by research grants from the Department of Biotechnology, Ministry of Science and Technology, Government of India. We thank Mary Jacob for expert assistance with histology, K. Preethi with FACS, and Vikram Mathews for liaising with us for human samples.

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Authors and Affiliations

  1. Center for Stem Cell Research, Christian Medical College, Bagayam, Vellore, 632002, India

    Sangeetha Hareendran, Salar Abbas & Prithi Rajan

  2. Department of Physiology, Christian Medical College, Bagayam, Vellore, 632002, India

    Solomon Sathishkumar

  3. GARP Research and Securities, Baltimore, MD, 21204, USA

    Alastair M. Mackay

  4. Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, 92037, USA

    Prithi Rajan

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  1. Sangeetha Hareendran
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  2. Solomon Sathishkumar
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  3. Salar Abbas
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  4. Alastair M. Mackay
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  5. Prithi Rajan
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Correspondence to Prithi Rajan.

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Sangeetha Hareendran and Solomon Sathishkumar equal contribution.

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Hareendran, S., Sathishkumar, S., Abbas, S. et al. A novel composition for the culture of human adipose stem cells which includes complement C3. Cytotechnology 62, 389–402 (2010). https://doi.org/10.1007/s10616-010-9301-1

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