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Cytotechnology

, Volume 62, Issue 4, pp 287–291 | Cite as

Induction of cell size vesicles from human lymphoma cell lines and their application to drug carriers

  • Makoto Yamanaka
  • Shigeki Nakamura
  • Aiko Inoue
  • Takashi Yasuda
  • Yuichi Inoue
  • Hiroharu Kawahara
JAACT Special Issue

Abstract

Sodium butyrate (NaB) induced the membrane enclosed cell size vesicles from several IgM producing cell lines. We considered the application of the cell-derived vesicles (CDVs) to drug delivery system (DDS) using the lung cancer specific IgM producing AE6 cell line. Microscopic observation showed that the DiI fluorescence labeled AE6 vesicles were incorporated into the lung cancer cell line A549. The anticancer drug, actinomycin D (actD), contained in AE6 and Ramos vesicles decreased the A549 cell viability to 46 and 62% of control without actD, respectively. The cytotoxic effect in AE6 vesicles was superior to that in the Ramos vesicles that have the lung cancer non-specific IgM on their surfaces. However, the result of the Ramos vesicles suggests that the surface molecules other than IgM may interact with the A549 cells. In our method for vesicle production, more specific and abundant antibodies mounted vesicles can be generated by transfection of their genes into cells followed by NaB treatment. These suggest that the CDVs may be useful for the development of a drug carrier for DDS.

Keywords

Anticancer Drug delivery system Lymphoma Sodium butyrate Vesicle 

Notes

Acknowledgments

We gratefully thank the kind provision of K562 cells by Dr. Mari Maeda Yamamoto, National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, Shizuoka, Japan.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Makoto Yamanaka
    • 1
  • Shigeki Nakamura
    • 2
  • Aiko Inoue
    • 2
  • Takashi Yasuda
    • 1
  • Yuichi Inoue
    • 2
  • Hiroharu Kawahara
    • 1
    • 2
  1. 1.Graduate School of Life Science and Systems EngineeringKyushu Institute of TechnologyKitakyushu-shiJapan
  2. 2.The Cell Engineering CenterKitakyushu National College of TechnologyKitakyushu-shiJapan

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