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Cytotechnology

, Volume 61, Issue 3, pp 145–152 | Cite as

Improved reproducibility in preparing precision-cut liver tissue slices

  • Martina Zimmermann
  • Johanna Lampe
  • Sebastian Lange
  • Irina Smirnow
  • Alfred Königsrainer
  • Claus Hann-von-Weyhern
  • Falko Fend
  • Michael Gregor
  • Michael Bitzer
  • Ulrich M. LauerEmail author
Technical Note

Abstract

Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great variability in the results, we sought to compare the reproducibility of tissue slices generated using the newly developed Leica VT1200 S vibrating blade microtome with Vibrocheck (LV) and the Krumdieck tissue slicer (KD) which has been the standard apparatus for this application so far. Liver samples from five different species (human, pig, cattle, rat, mouse) were cut and the reproducibility of slice thickness was analyzed by cross sectioning the PCLS. The quality of the sliced tissue was determined via measurement of the ATP content. As a result, we found an improved accuracy and reproducibility of rat, mouse and human tissue slices using the new Leica vibrating blade microtome.

Keywords

Primary culture Precision-cut liver slices (PCLS) Leica VT1200 S vibrating blade microtome Krumdieck tissue slicer 

Notes

Acknowledgment

We are grateful to Leica Biosystems Nussloch GmbH for the provision of a research grant.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Martina Zimmermann
    • 1
  • Johanna Lampe
    • 1
  • Sebastian Lange
    • 1
  • Irina Smirnow
    • 1
  • Alfred Königsrainer
    • 2
  • Claus Hann-von-Weyhern
    • 3
  • Falko Fend
    • 3
  • Michael Gregor
    • 1
  • Michael Bitzer
    • 1
  • Ulrich M. Lauer
    • 1
    Email author
  1. 1.Department of Gastroenterology and HepatologyUniversity Hospital TuebingenTuebingenGermany
  2. 2.Department of General, Visceral and Transplant SurgeryUniversity Hospital TuebingenTuebingenGermany
  3. 3.Department of PathologyUniversity Hospital TuebingenTuebingenGermany

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