Abstract
To develop an investigative tool for the study of human osteosarcoma (OSA), we established a human OSA cell line, namely, SOSP-9607, which exhibits a potential for spontaneous pulmonary metastasis. Subsequently, we screened two related sublines (F5M2 and F4) that have different pulmonary metastatic potentials. An in vivo orthotopic transplantation assay confirmed spontaneous pulmonary metastasis in all mice (100%) transplanted with the more aggressive OSA cells (F5M2) and a lesser degree of metastases with smaller nodules in 33.3% mice transplanted with the less aggressive OSA cell subline (F4). In mice transplanted with F5M2 cells, death from metastasis occurred at a median of 71 days; however, in mice transplanted with F4, no death occurred even after 120 days. Therefore, the F5M2 and F4 sublines, which originated from the same parent cell line, differed with respect to metastasis-related properties such as proliferating ability and invasiveness. Hence, these well-characterized human OSA sublines can be used as valuable models for comparative studies of genetic determinants of OSA in the future.
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Acknowledgments
This work is supported by two grants from the National Natural Science Foundation of China (No. 30330610 and No. 30873027). We would like to thank Professor Qiu Xiuchun for his technical help and Zhao Hong for his efforts in taking care of animals. We also thank Dr. Ji Zhengang for his valuable advice.
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Xiang Chen and Tong-Tao Yang contributed equally to this work.
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Chen, X., Yang, TT., Wang, W. et al. Establishment and characterization of human osteosarcoma cell lines with different pulmonary metastatic potentials. Cytotechnology 61, 37–44 (2009). https://doi.org/10.1007/s10616-009-9239-3
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DOI: https://doi.org/10.1007/s10616-009-9239-3