Liquiritin potentiate neurite outgrowth induced by nerve growth factor in PC12 cells
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Neurite outgrowth and neuronal differentiation play a crucial role in the development of the nervous system. Understanding of neurotrophins induced neurite outgrowth was important to develop therapeutic strategy for axon regeneration in neurodegenerative diseases as well as after various nerve injuries. It has been reported that extension of neurite and differentiation of sympathetic neuron-like phenotype was modulated by nerve growth factor (NGF) in PC12 cells. In this study, NGF mediated neurite outgrowth was investigated in PC12 cells after liquiritin exposure. Liquiritin is a kind of flavonoids that is extracted from Glycyrrhizae radix, which is frequently used to treat injury or swelling for its life-enhancing properties as well as detoxification in traditional Oriental medicine. The result showed that liquiritin significantly promotes the neurite outgrowth stimulated by NGF in PC12 cells in dose dependant manners whereas the liquiritin alone did not induce neurite outgrowth. Oligo microarray and RT-PCR analysis further clarified that the neurotrophic effect of liquiritin was related to the overexpression of neural related genes such as neurogenin 3, neurofibromatosis 1, notch gene homolog 2, neuromedin U receptor 2 and neurotrophin 5. Thus, liquiritin may be a good candidate for treatment of various neurodegenerative diseases such as Alzheimer’s disease or Parkinson’s disease.
KeywordsLiquiritin PC12 cells Neurite NGF Microarray
- Chen LM, Lu QJ, Satoshi I, Bian GX, Chen ZH, Wen LQ (2006) Establishment of a reporter gene-based cell screening model for discovering new agonists of estrogen receptor beta subtype. Yao Xue Xue Bao 41:721–726Google Scholar
- Hibasami H, Iwase H, Yoshioka K, Takahashi H (2005) Glycyrrhizin induces apoptosis in human stomach cancer KATO III and human promyelotic leukemia HL-60 cells. Int J Mol Med 16:233–236Google Scholar
- Okamura N, Miki H, Orii H, Masaoka Y, Yamashita S, Kobayashi H, Yagi A (1999) Simultaneous high-performance liquid chromatographic determination of puerarin, daidzin, paeoniflorin, liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizin in Kampo medicines. J Pharm Biomed Anal 19:603–612CrossRefGoogle Scholar
- Xu P, Pu PY, Kang CS, Jia ZF, Zhou X, Wang GX (2008) Differential expression of Notch1 and Notch2 in astrocytoma and medulloblastoma. Zhonghua Bing Li Xue Za Zhi 37:450–453Google Scholar