Skip to main content

Advertisement

SpringerLink
Log in

Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells

  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

Periosteum-derived cells (PDCs) are being extensively studied as potential tissue engineering seed cells and have demonstrated tremendous promise to date. There is convincing evidence that culture medium could modulate the biological behavior of cultured cells. In this study, we investigate the effects of DMEM (low glucose) and RPMI 1640 on cell growth and cell differentiation of PDCs in vitro. PDCs isolated from Beagle dogs were maintained in DMEM and RPMI 1640, respectively. Then, the cell migration rate of periosteum tissues was analyzed. PDCs of the third passage were harvested for the study of proliferation and osteogenic activity. Proliferation was detected by MTT assay. Alkaline phosphatase activity and mineralized nodules were measured to investigate osteogenic differentiation. Our data demonstrated that DMEM induced alkaline phosphatase activity and strongly stimulated matrix mineralization in cell culture, while similar cell migration rates and proliferation behaviors were observed in the two culture conditions. Interestingly, the osteogenic differentiation of PDCs could be enhanced in DMEM compared with that in RPMI 1640. Thus, it can be ascertained that DMEM may serve as a suitable culture condition allowing osteogenic differentiation of dog PDCs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Agata H, Asahina I, Yamazaki Y, Uchida M, Shinohara Y, Honda MJ, Kagami H, Ueda M (2007) Effective bone engineering with periosteum-derived cells. J Dent Res 86(1):79–83. doi:10.1177/154405910708600113

    Article  CAS  Google Scholar 

  • Chen F, Hui JH, Chan WK, Lee EH (2003) Cultured mesenchymal stem cell transfers in the treatment of partial growth arrest. J Pediatr Orthop 23(4):425–429. doi:10.1097/00004694-200307000-00002

    Article  Google Scholar 

  • Choi YS, Noh SE, Lim SM, Lee CW, Kim CS, Im MW, Lee MH, Kim DI (2007) Multipotency and growth characteristic of periosteum-derived progenitor cells for chondrogenic, osteogenic, and adipogenic differentiation. Biotechnol Lett 30(4):593–601. doi:10.1007/s10529-007-9584-2

    Article  CAS  Google Scholar 

  • De Bari C, Dell’Accio F, Vanlauwe J, Eyckmans J, Khan IM, Archer CW, Jones EA, McGonagle D, Mitsiadis TA, Pitzalis C, Luyten FP (2006) Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis. Arthritis Rheum 54(4):1209–1221. doi:10.1002/art.21753

    Article  CAS  Google Scholar 

  • Declercq HA, Ridder LI, Cornelissen MJ (2005a) Isolation and osteogenic differentiation of rat periosteum-derived cells. Cytotechnology 49:39–50. doi:10.1007/s10616-005-5167-z

    Article  Google Scholar 

  • Declercq HA, Verbeeck RM, De Ridder LI, Schacht EH, Cornelissen MJ (2005b) Calcification as an indicator of osteoinductive capacity of biomaterials in osteoblastic cell cultures. Biomaterials 26(24):4964–4974. doi:10.1016/j.biomaterials.2005.01.025

    Article  CAS  Google Scholar 

  • Eklou-Kalonji E, Denis I, Lieberherr M, Pointillart A (1998) Effects of extracellular calcium on the proliferation and differentiation of porcine osteoblasts in vitro. Cell Tissue Res 292(1):163–171. doi:10.1007/s004410051046

    Article  CAS  Google Scholar 

  • Groeneveld MC, Everts V, Beertsen W (1994) Formation of afibrillar acellular cementum-like layers induced by alkaline phosphatase activity from periodontal ligament explants maintained in vitro. J Dent Res 73(10):1588–1592

    CAS  Google Scholar 

  • Hasegawa N, Kawaguchi H, Hirachi A, Takeda K, Mizuno N, Nishimura M, Koike C, Tsuji K, Iba H, Kato Y, Kurihara H (2006) Behavior of transplanted bone marrow-derived mesenchymal stem cells in periodontal defects. J Periodontol 77(6):1003–1007. doi:10.1902/jop.2006.050341

    Article  Google Scholar 

  • Hildebrandt C, Büth H, Thielecke H (2009) Influence of cell culture media conditions on the osteogenic differentiation of cord blood-derived mesenchymal stem cells. Ann Anat 191(1):23–32. doi:10.1016/j.aanat.2008.09.009

    Article  Google Scholar 

  • Hou LT, Li TI, Liu CM, Liu BY, Liu CL, Mi HW (2007) Modulation of osteogenic potential by recombinant human bone morphogenic protein-2 in human periodontal ligament cells: effect of serum, culture medium, and osteoinductive medium. J Periodontal Res 42(3):244–252. doi:10.1111/j.1600-0765.2006.00940.x

    Article  CAS  Google Scholar 

  • Jansen EJ, Emans PJ, Guldemond NA, van Rhijn LW, Welting TJ, Bulstra SK, Kuijer R (2008) Human periosteum-derived cells from elderly patients as a source for cartilage tissue engineering? J Tissue Eng Regen 2(6):331–339. doi:10.1002/term.100

    Article  CAS  Google Scholar 

  • Kim WS, Kim HK (2005) Tissue engineered vascularized bone formation using in vivo implanted osteoblast-polyglycolic acid scaffold. J Korean Med Sci 20(3):479–482

    Article  CAS  Google Scholar 

  • Kim HS, Park JW, Yeo SI, Choi BJ, Suh JY (2006) Effects of high glucose on cellular activity of periodontal ligament cells in vitro. Diabetes Res Clin Pract 74(1):41–47. doi:10.1016/j.diabres.2006.03.034

    Article  CAS  Google Scholar 

  • Li YM, Schilling T, Benisch P, Zeck S, Meissner-Weigl J, Schneider D, Limbert C, Seufert J, Kassem M, Schütze N, Jakob F, Ebert R (2007) Effects of high glucose on mesenchymal stem cell proliferation and differentiation. Biochem Biophys Res Commun 363(1):209–215. doi:10.1016/j.bbrc.2007.08.161

    Article  CAS  Google Scholar 

  • Li H, Yan F, Lei L, Li Y, Xiao Y (2008) Application of autologous cryopreserved bone marrow mesenchymal stem cells for periodontal regeneration in dogs. Cells Tissues Organs. doi:10.1159/000166547

  • Lin NH, Grontho S, Bartold PM (2008) Stem cells and periodontal regeneration. Aust Dent J 53(2):108–121. doi:10.1111/j.1834-7819.2008.00019.x

    Article  Google Scholar 

  • Liu YK, Lu QZ, Pei R, Ji HJ, Zhou GS, Zhao XL, Tang RK, Zhang M (2009) The effect of extracellular calcium and inorganic phosphate on the growth and osteogenic differentiation of mesenchymal stem cells in vitro: implication for bone tissue engineering. Biomed Mater 4(2):25004. doi:10.1088/1748-6041/4/2/025004

    Article  CAS  Google Scholar 

  • Lopez-Cazuax S, Bluteau G, Magne D, Lieubeau B, Guicheux J, Alliot-Litht B (2006) Culture medium modulates the behaviour of human dental pulp-derived cells. Eur Cell Mater 11:35–42

    Google Scholar 

  • Maeno S, Niki Y, Matsumoto H, Morioka H, Yatabe T, Funayama A, Toyama Y, Taguchi T, Tanaka J (2005) The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials 26(23):4847–4855. doi:10.1016/j.biomaterials.2005.01.006

    Article  CAS  Google Scholar 

  • Matsumoto A (1995) The effect of cell environment on osteoblastic function. Nippon Yakurigaku Zasshi 105(5):273–283. doi:10.1254/fpj.105.273

    CAS  Google Scholar 

  • Mizuno H, Hata K, Kojima K, Bonassar LJ, Vacanti CA, Ueda M (2006) A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum. Tissue Eng 12(5):1227–1335. doi:10.1089/ten.2006.12.1227

    Article  Google Scholar 

  • Perka C, Schultz O, Spitzer RS, Lindenhayn K, Burmester GR, Sittinger M (2000) Segmental bone repair by tissue-engineered periosteal cell transplants with bioresorbable fleece and fibrin scaffolds in rabbits. Biomaterials 21(11):1145–1153. doi:10.1016/S0142-9612(99)00280-X

    Article  CAS  Google Scholar 

  • Robert DG, Leslie AL (1997) A laboratory manual. Cold Spring Harbor Laboratory Press, Chicago

    Google Scholar 

  • Steiner GG, Kallet MP, Steiner DM, Roulet DN (2007) The inverted periosteal graft. Compend Contin Educ Dent 28(3):154–161

    Google Scholar 

  • Stolzing A, Coleman N, Scutt A (2006) Glucose-induced replicative senescence in mesenchymal stem cells. Rejuvenation Res 9(1):31–35. doi:10.1089/rej.2006.9.31

    Article  CAS  Google Scholar 

  • Youn I, Suh JK, Nauman EA, Jones DG (2005) Differential phenotypic characteristics of heterogeneous cell population in the rabbit periosteum. Acta Orthop 76(3):442–450

    Google Scholar 

  • Youshimura Y, Hisada Y, Suzuki K, Deyama Y, Matsumoto A (1996) Effect of a low-calcium environment on alkaline phosphatase activity in embryonic rat calvarial bone cells in culture. Arch Oral Biol 41:41–45. doi:10.1016/0003-9969(95)00104-2

    Article  Google Scholar 

  • Zhang C, Hu YY, Cui FZ, Zhang SM, Ruan DK (2006) A study on a tissue-engineered bone using rhBMP-2 induced periosteal cells with a porous nano-hydroxyapatite/col-lagen/poly (l-lactic acid) scaffold. Biomed Mater 1(2):56–62. doi:10.1088/1748-6041/1/2/002

    Article  CAS  Google Scholar 

  • Zhang Y, Song J, Shi B, Wang Y, Chen X, Huang C, Yang X, Xu D, Cheng X, Chen X (2007) Combination of scaffold and adenovirus vectors expressing bone morphogenetic protein-7 for alveolar bone regeneration at dental implant defects. Biomaterials 28(31):4635–4642. doi:10.1016/j.biomaterials.2007.07.009

    Article  CAS  Google Scholar 

  • Zheng YX, Ringe J, Liang Z, Loch A, Chen L, Sittinger M (2006) Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum. J Zhejiang Univ Sci B 7(10):817–824. doi:10.1631/jzus.2006.B0817

    Article  Google Scholar 

  • Zhu SJ, Choi BH, Huh JY, Jung JH, Kim BY, Lee SH (2006) A comparative qualitative histological analysis of tissue-engineered bone using bone marrow mesenchymal stem cells, alveolar bone cells, and periosteal cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101(2):164–169. doi:10.1016/j.tripleo.2005.04.006

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Natural Science Foundation of China (30471892), the Natural Science Foundation of Fujian Province, China (2008J0085), and the Key Disciplines Research Foundation from School of Stomatology, Fujian Medical University (No. 2008-39). The authors thank Hong Chen and Lei Ma for technical assistance.

Author information

Authors and Affiliations

  1. Department of Periodontology, Fujian Medical University, 246 Yangqiao Zhong Road, Fuzhou, 350002, China

    Xiaohong Wu, Minkui Lin, Yanfen Li, Xin Zhao & Fuhua Yan

Authors
  1. Xiaohong Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Minkui Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanfen Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xin Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fuhua Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fuhua Yan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wu, X., Lin, M., Li, Y. et al. Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells. Cytotechnology 59, 103–111 (2009). https://doi.org/10.1007/s10616-009-9200-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-009-9200-5

Keywords

Search

Navigation