, Volume 59, Issue 1, pp 45–53 | Cite as

Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells

  • Junkyu Han
  • Terence P. N. Talorete
  • Parida Yamada
  • Hiroko IsodaEmail author
Special Issue JAACT


Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxytyrosol. In this study, oleuropein and hydroxytyrosol, major phenolic compound of olive oil, was studied for its effects on growth in MCF-7 human breast cancer cells using assays for proliferation (MTT assay), cell viability (Guava ViaCount assay), cell apoptosis, cellcycle (flow cytometry). Oleuropein or hydroxytyrosol decreased cell viability, inhibited cell proliferation, and induced cell apoptosis in MCF-7 cells. Result of MTT assay showed that 200 μg/mL of oleuropein or 50 μg/mL of hydroxytyrosol remarkably reduced cell viability of MCF-7 cells. Oleuropein or hydroxytyrosol decrease of the number of MCF-7 cells by inhibiting the rate of cell proliferation and inducing cell apoptosis. Also hydroxytyrosol and oleuropein exhibited statistically significant block of G1 to S phase transition manifested by the increase of cell number in G0/G1 phase.


MCF-7 cells Oleuropein Hydroxytyrosol Apoptosis 


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Junkyu Han
    • 1
  • Terence P. N. Talorete
    • 1
  • Parida Yamada
    • 1
  • Hiroko Isoda
    • 1
    Email author
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukuba, IbarakiJapan

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