, Volume 59, Issue 1, pp 31–44 | Cite as

CD90 Expression on human primary cells and elimination of contaminating fibroblasts from cell cultures

  • Lynn KisselbachEmail author
  • Michael Merges
  • Alexis Bossie
  • Ann Boyd
Method in Cell Science


Cluster Differentiation 90 (CD90) is a cell surface glycoprotein originally identified on mouse thymocytes. Although CD90 has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells, the knowledge about the levels of CD90 expression on different cell types, including human primary cells, is limited. The goal of this study was to identify CD90 as a human primary cell biomarker and to develop an efficient and reliable method for eliminating unwanted or contaminating fibroblasts from human primary cell cultures suitable for research pursuant to cell based therapy technologies.


CD90 expression Human primary cell cultures Elimination of contaminating fibroblasts Magnetic bead cell separation 



This work was supported and by Lonza Walkersville, Inc. and Hood College; all support is greatly appreciated. We thank Kim Warren, Ali Mohamed, Kim Roberts, Joseph Finny and Chris Elias for all of their help and support throughout this study.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Lynn Kisselbach
    • 1
    Email author
  • Michael Merges
    • 1
  • Alexis Bossie
    • 2
  • Ann Boyd
    • 3
  1. 1.Department of Cell TherapyLonza Walkersville, Inc.WalkersvilleUSA
  2. 2.Department of Media ServicesLonza Walkersville, Inc.WalkersvilleUSA
  3. 3.Department of BiologyHood CollegeFrederickUSA

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